MAP1LC3B Recombinant Monoclonal Antibody

說明書

中文名稱：

MAP1LC3B重組抗體
貨號：

CSB-RA551427A0HU
規格：

￥1320
圖片：
Western Blot
Positive WB detected in: HepG2 whole cell lysate,U87 whole cell lysate,RAW264.7 whole cell lysate,U251 whole cell lysate,Hela whole cell lysate
All lanes: LC3B antibody at 1:500
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 15 kDa
Observed band size: 15 kDa

Overlay Peak curve showing Hela cells stained with CSB-RA551427A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1μg/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1μg/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品描述：

CSB-RA551414A0HU MAP1LC3B重組單克隆抗體是針對自噬關鍵標志物LC3B蛋白研發的高特異性檢測工具。該抗體靶向的MAP1LC3B（微管相關蛋白1輕鏈3β）是自噬體形成過程中的核心組分，其脂化形式LC3-II可作為自噬流監測的重要分子標記。經嚴格驗證，本產品在蛋白免疫印跡（WB）中可清晰識別內源性LC3B蛋白，推薦使用1:500-1:2000稀釋梯度；在流式細胞術（FC）分析中可有效檢測細胞內的LC3B表達動態，建議采用1:50-1:200工作濃度。該重組抗體采用單克隆技術制備，具有高批次一致性和低交叉反應性，適用于探究細胞自噬機制、評估藥物或環境應激誘導的自噬活性變化等研究場景，特別是在腫瘤生物學、神經退行性疾病模型及代謝調控等領域的體外實驗中，可為研究人員提供穩定可靠的檢測支持。
Uniprot No.：

Q9GZQ8
基因名：

MAP1LC3B
別名：

Microtubule-associated proteins 1A/1B light chain 3B (Autophagy-related protein LC3 B) (Autophagy-related ubiquitin-like modifier LC3 B) (MAP1 light chain 3-like protein 2) (MAP1A/MAP1B light chain 3 B) (MAP1A/MAP1B LC3 B) (Microtubule-associated protein 1 light chain 3 beta), MAP1LC3B, MAP1ALC3
反應種屬：

Human
免疫原：

A synthesized peptide derived from Human MAP1LC3B
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

7D11
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產品提供形式：

Liquid
應用范圍：

ELISA, WB, FC

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:2000
FC	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. In response to cellular stress and upon mitochondria fission, binds C-18 ceramides and anchors autophagolysosomes to outer mitochondrial membranes to eliminate damaged mitochondria. While LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway. Through its interaction with the reticulophagy receptor TEX264, participates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover.
基因功能參考文獻：
1. Study demonstrated that the expression of LC3B was upregulated in 4nitroquinoline1oxideinduced oral carcinogenesis, accompanied with myeloidderived suppressor cells and regulatory T cells accumulation. PMID: 30272335
2. High p62 cytoplasmic expression on its own (p PMID: 29897944
3. Here the authors present evidence for phosphorylation-driven regulation of the Nix:LC3B interaction. Isothermal titration calorimetry and NMR indicate a ~100 fold enhanced affinity of the serine 34/35-phosphorylated Nix LC3-interacting region (LIR) to LC3B and formation of a very rigid complex compared to the non-phosphorylated sequence. PMID: 28442745
4. The results suggest that autophagy-associated proteins LC3A, LC3B, and Beclin-1 might be potential biomarkers for subclassification, differentiation, and local metastasis in primary lung tumor. PMID: 29545906
5. Simultaneous high expression of LC3B (and ULK1) had a poorer survival rate in hepatocellular carcinoma patients. PMID: 29091866
6. LC3B and ESRRA might be a useful prognostic factor in patients with Muscle-invasive bladder cancer. The co-expression of LC3B and ESRRA might be a prognostic and therapeutic target for patients with bladder cancer. PMID: 29599373
7. High levels of LC3B is associated with non-small cell lung cancer. PMID: 28558758
8. LC3b was significantly overexpressed in malignant compared to benign prostate tissue. However, positive LC3b immunoreactivity in PCa, as a marker of increased autophagy, was independently associated with a reduced disease-specific mortality. PMID: 28423666
9. Our results suggested that activated Akt/mTOR-autophagy may have a role in the local T cell-mediated immunoregulatory mechanism of oral lichen planus (OLP). LC3B might be a valuable marker to monitor the disease severity of OLP. PMID: 28482233
10. presence of LC3B puncta and HMGB1 expression in malignant cells correlate with the immune infiltrate in breast cancer PMID: 26979828
11. the L341V mutation limits the critical step of SQSTM1 recruitment to the phagophore PMID: 27158844
12. LC3B and p62 have roles in autophagy in esophageal adenocarcinoma PMID: 27250034
13. Data indicate that tubule-associated protein 1 light chain 3 beta (LC3B) can be potentially useful for identifying autophagosomes and differentiating their developmental stages. PMID: 28506764
14. analysis of the RavZ and LC3 complex reveals the mechanism for deconjugation of LC3 on the phagophore membrane PMID: 27791457
15. SQSTM1 is ubiquitinated by NEDD4 while LC3 functions as an activator of NEDD4 ligase activity. PMID: 28085563
16. Cardiolipin interaction with various Atg8 human orthologs, namely LC3B, GABARAPL2 and GABARAP, was investigated. PMID: 27764541
17. Insights into links between autophagy and the ubiquitin system showed that LC3B-binding can steer intrinsic NEDD4 E3 ligase activity. PMID: 28470758
18. Determined is the three-dimensional crystal structures of LC3B in complex with three different LIR motifs of RavZ from Legionella pneumophila, an intracellular pathogen that can manipulate the host autophagy system. PMID: 28668392
19. We found that 25-epi Ritterostatin GN1N induced cell death in melanoma cells at nanomolar concentrations, and this cell death was characterized by inhibition of GRP78 expression, increased expression of the ER stress marker CHOP, loss of mitochondrial membrane potential, and lipidation of the autophagy marker protein LC3B. PMID: 28393217
20. Double IF showed the co-localization of AQP5 and LC3B on BafA1-treated heated cells. In conclusion, we demonstrated that heat shock decreased AQP5 on cellular membranes and in the cytoplasm by activating autophagic degradation, and heat shock and AQP5 knockdown exerted similar anticancer effects, suggesting that heat shock exerts anticancer effects via the autophagic degradation of AQP5. PMID: 28358429
21. structural and biochemical results reveal a working model for the specific recognition of FUNDC1 by LC3B and imply that the reversible phosphorylation modification of mitophagy receptors may be a switch for selective mitophagy. PMID: 27757847
22. Low expression of MAP1LC3B is associated with lymph node metastasis in gastric cancer. PMID: 27655288
23. Poly C binding protein 1 represses autophagy through downregulation of LC3B to promote tumor cell apoptosis in starvation PMID: 26880484
24. BAG3 maintains the basal amount of LC3B protein by controlling the translation of its mRNA in HeLa and HEK293 cells. PMID: 26654586
25. Among the 101 patients, the frequency of high expression of beclin-1 was 31.7% (32/101) and that of LC3b was 46.5% (47/101). A pathologic complete response was inversely associated with LC3b expression (P = 0.003) and alterations in the expression of autophagy-related proteins PMID: 26965179
26. Collectively, our findings indicate that MIR494 reduces cell survival in 769-P renal cancer cells which is accompanied by increased lipid droplet formation (which occurs in a LC3B-dependent manner) and mitochondrial changes. PMID: 26794413
27. Data show CGK733 induced microtubule associated protein LC3B formation upstream of AMP-activated protein kinase and protein kinase RNA-like endoplasmic reticulum kinase/CCAAT-enhancer-binding protein homologous protein pathways and p21 Cip1 expression. PMID: 26486079
28. the combined positivity for LC3B(+) puncta and nuclear HMGB1 is a positive predictor for longer BC survival PMID: 26506894
29. In microsatellite stable carcinomas, the level of LC3B-II expression was higher than that in the microsatellite unstable carcinomas. PMID: 26502823
30. Loss of HPS1 protein results in impaired autophagy that is restored by exogenous LC3B and that defective autophagy might therefore play a critical role in the development and progression of Hermansky-Pudlak syndrome. PMID: 26719147
31. mRNA levels of MAP1LC3B, an autophagic marker, showed a 5-fold decrease in symptomatic samples. PMID: 25503069
32. LC3B may promote the migration and invasion of EOC cells by affecting the cytoskeleton via the RhoA pathway. PMID: 25607473
33. This study unveils that HIV-1 Vif inhibits autophagy via interaction with LC3B independently of its action on APOBEC3G and, therefore, suggest a new function of this viral protein in restricting innate antiviral mechanisms. PMID: 25490467
34. Data show that interaction between promyelocytic leukemia protein (PML) and microtubule-associated protein light chain 3 (LC3) contributes to cell growth inhibition function of PML. PMID: 25419843
35. investigated the expression of autophagy-related markers microtubule-associated protein IA/IB light chain 3 (LC3) and p62/sequestosome-1 (p62), in cutaneous squamous cell carcinoma specimens and assessed their correlation to clinicopathological factors PMID: 24690104
36. When not bound to autophagosomes, LC3B associates with a multicomponent complex with an effective size of ~500 kDa in the cytoplasm. PMID: 24646892
37. positive fibroblastic LC3B correlates with lower invasion, and low expression of fibroblastic Cav-1 is a novel predictor of poor GC prognosis. PMID: 23203033
38. high expression of LC3B, correlated with vascular invasion and lymph node metastasis, might be a novel prognostic biomarker and would be a potential therapy target for HCC. PMID: 25256671
39. High intensity of LC3B staining was predictive of poor prognosis. PMID: 24900981
40. elevating the levels of TSC1 (tuberous sclerosis complex) and TSC2 and inactivating MTOR and RPS6KB/p70S6K, causing cleaved MAP1LC3B levels to increase PMID: 24113030
41. High cytoplasmic p62 expression accompanied with either a low or high LC3B expression. PMID: 24983366
42. Data indicate that high cytoplasmic microtubule-associated protein 1 light chain LC3A, LC3B, Beclin 1 and p62/SQSTM1 expressions were independently linked with the Gleason score. PMID: 23787295
43. LC3B can be used as a prognostic marker in patients with non-pCR after NCT for breast cancer, which highlights the importance of autophagy in the biologic behavior of chemoresistant cancer cells PMID: 24141623
44. knockdown of LC3B but not GABARAPs resulted in significant accumulation of p62/Sqstm1, one of the selective substrates for autophagy PMID: 24582747
45. The results of this study identify a new physiological role for the PSF-LC3B axis as a potential endogenous modulator of colon cancer treatment. PMID: 24288667
46. these data indicated that LC3B-II deacetylation, which was partly mediated by HDAC6, is involved in autophagic degradation during serum starvation. PMID: 24220335
47. Beclin-1 and LC3-II are downregulated in hypopharyngeal squamous cell carcinoma patients, and their aberrant expression correlates with poor prognosis. PMID: 23935917
48. NMR and crystal structures of the autophagy modifier LC3B in complex with the LC3 interaction region of optineurin. PMID: 23805866
49. These preliminary results demonstrated that high LC3B expression was associated with lymph node and distant metastasis in triple-negative breast cancer PMID: 23371253
50. Data show that VPRBP (viral protein R-binding protein)-LC3B (light-chain 3B)/p62(SQSTM1) were in the same protein complex. PMID: 22963397
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亞細胞定位：

Cytoplasm, cytoskeleton. Endomembrane system; Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor.
蛋白家族：

ATG8 family
組織特異性：

Most abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver.
數據庫鏈接：

HGNC: 13352

OMIM: 609604

KEGG: hsa:81631

STRING: 9606.ENSP00000268607

UniGene: Hs.356061

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