LAMC1 Recombinant Monoclonal Antibody

說(shuō)明書

中文名稱：

LAMC1 Recombinant Monoclonal Antibody
貨號(hào)：

CSB-RA053648A0HU
規(guī)格：

￥1320
圖片：
Immunocytochemical staining of HAP-1 cells with Laminin subunit gamma 1 antibody. Nuclei were stained blue with DAPI; Laminin subunit gamma 1 was stained magenta with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Flow cytometric analysis of Laminin subunit gamma 1 expression in HAP-1 cells using Laminin subunit gamma 1 antibody. Green, isotype control; red, Laminin subunit gamma 1.

Western blotting analysis using Laminin subunit gamma 1 antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Laminin subunit gamma 1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
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靶點(diǎn)詳情

產(chǎn)品詳情

Uniprot No.：

P11047
基因名：

LAMC1
別名：

LAMC1; Laminin Subunit Gamma 1; Laminin B2 Chain; LAMB2; Laminin, Gamma 1 (Formerly LAMB2); Laminin-10 Subunit Gamma; Laminin-11 Subunit Gamma; Laminin Subunit Gamma-1; S-Laminin Subunit Gamma; Laminin-2 Subunit Gamma; Laminin-3 Subunit Gamma; Laminin-4 Subunit Gamma; Laminin-6 Subunit Gamma; Laminin-7 Subunit Gamma; Laminin-8 Subunit Gamma; Laminin-9 Subunit Gamma; S-LAM Gamma; Laminin-1 Subunit Gamma
反應(yīng)種屬：

Human, Rat
免疫原：

Recombinant Human LAMC1 protein
免疫原種屬：

Homo sapiens (Human)
標(biāo)記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號(hào)：

18F1
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, WB, FC, ICC

推薦稀釋比：

Application	Recommended Dilution
WB	1:1000-1:5000
FC	1:200-1:2000
ICC	1:100-1:1000

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Flow cytometry (FC) Protocol
儲(chǔ)存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
基因功能參考文獻(xiàn)：
1. Polymorphism in the ERalpha gene is associated with an increased risk for advanced Pelvic Organ Prolapse. However, polymorphism in the LAMC1 gene does not seem to be associated with such risk. PMID: 29241914
2. We conclude that LAMC1 mRNA acts as a trans regulator to stimulate CD151 expression by competing for miR-124 binding in hepatocellular carcinoma cells. PMID: 28524360
3. Further gene expression analyses demonstrated significantly higher expression of LAMC1 gene in colorectal cancer (CRC) tumor tissues than that in adjacent non-cancerous tissues (P = 0.0004). These findings strongly suggest that the functional SNP located at TFBSs, rs6695837 might contribute to CRC susceptibility, and the exact biological mechanism awaits further research. PMID: 28039327
4. The rs780094 [1.34 (1.21-1.49); p = 8.57 x 10(-8) ] on chr 2 at the glucokinase regulatory protein (GCKR) locus was similarly significantly associated, while the rs10911205 [1.29 (1.16-1.44); p = 3.52 x 10(-6) ] on chr1 at the laminin subunit gamma-1 (LAMC1) locus showed suggestive association with disease. PMID: 27599772
5. Our results show that miR-181a is down-regulated in glioblastoma multiforme (GBM) patients. The three target genes, ANGPT2, ARHGAP18 and LAMC1, are negatively correlated with the expression of miR-181a. Moreover, high expression of ANGPT2 or LAMC1 together with large size of GBM is correlated with a shorter median overall survival PMID: 27176932
6. miR-506 acts as a tumor suppressor and inhibits malignancy of colorectal cancer cells through directly targeting LAMC1. PMID: 27993882
7. Case Report: Anti-laminin-gamma 1 pemphigoid in patient with generalized pustular psoriasis and psoriasis vulgaris. PMID: 26074471
8. The identification of laminin gamma 1 (LAMC1) and myeloid cell leukemia 1 (MCL1) as direct targets of miR-22 and miR-29a, respectively, suggested a tumor-suppressive role of these miRNAs. PMID: 26052614
9. LAMC1 may contribute to the development and progression of uterine carcinoma, likely through enhancing tumor cell motility and invasion. PMID: 26343160
10. Case Report: occurrence of anti-laminin-gamma1 pemphigoid in a case of autosomal recessive congenital ichthyosis. PMID: 24733442
11. Data showed that LAMC1 expression is directly regulated by miR-29s. PMID: 24820027
12. These findings document a pathogenic potential of antibodies targeting the laminin gamma1 N-terminus. PMID: 24300951
13. carbohydrate moiety (alpha-Gal) on laminin gamma-1 and collagen alpha-1 (VI) chain are possibly common IgE-reactive proteins in the Japanese patients with beef allergy PMID: 24180678
14. results demonstrate that mutation in either NID1 or LAMC1 disrupts the interaction NID1-LAMC1 complex; findings implicate the extracellular matrix in the pathogenesis of Dandy-Walker spectrum disorders PMID: 23674478
15. LAMC1 polymorphism could be a protective factor for SLE. PMID: 23039241
16. Results suggest that laminin gamma1 expression is associated with meningioma grades and could play a role in enhancing tumor invasion. PMID: 23053286
17. Genetic polymorphism of LAMC1 gene is associated in the pathogenesis of colorectal cancers. PMID: 23266556
18. Letter: a small but distinct subgroup of both systemic and cutaneous lupus erythematosus patients has antibodies against the C-terminus of laminin gamma1. PMID: 22968451
19. Autoantibodies in anti-p200 pemphigoid sera are pathogenic while pathogenicity is not mediated by autoantibodies against laminin gamma1. PMID: 22911854
20. Overexpression of laminin gamma1 immunoreactivity and alteration of its staining pattern may serve as a useful biomarker in serous tubal intraepithelial carcinoma. PMID: 22892598
21. Mutations in the coding sequence of LAMC1 and DLGH1 may not be associated with Mayer-Rokitansky-Kuster-Hauser Syndrome. PMID: 22377151
22. Single-nucleotide polymorphisms (SNPs) representing 99% coverage of LAMC1 were genotyped. There was no association between SNP rs10911193 and advanced pelvic organ prolapse. There was a trend toward significance for SNPs rs1413390, rs20563, and rs20558. PMID: 22342894
23. LAMC1 is significantly associated with premature ovarian failure, and specifically, possession of at least one HT1 was associated with susceptibility to premature ovarian failure. PMID: 22321639
24. Laminin-5 gamma2 chain and SPARC may play roles in the progression of esophageal squamous cell carcinoma and their simultaneous expression is correlated with poorer prognosis. PMID: 21192846
25. The nano-structure of the laminin gamma-1 short arm was determined. In solution, it adapts an extended conformation. PMID: 20688161
26. Several LAMC1 single nucleotide polymorphisms exist with significant differences between African American and Caucasian women, but no association found between any LAMC1 variant and advanced pelvic organ prolapse. PMID: 20223449
27. Enhanced and specialized expression patterns of alpha 1 and gamma 1 laminins distinctly associate these two laminins with the Alzheimer disease. PMID: 12111806
28. The neurite outgrowth domain of gamma 1 laminin accumulates heavily in the floor plate region, in the notochord and in GFAP-immunoreactive glial fibers of the embryonic spinal cord. PMID: 12526023
29. Basement membrane formation is inhibited by a nidogen-laminin gamma1-chain fragment in skin-organotypic cultures. PMID: 15159456
30. Expression of LAMC1 in glioblastoma is inhibited by indomethacin. Indomethacin inhibits cyclooxygenases and is an NSAID drug. PMID: 15561105
31. a polymorphism in the promoter of LAMC1 may increase the susceptibility to early-onset pelvic organ prolapse PMID: 17021862
32. gamma1 laminin is selectively overexpressed in reactive astrocytes of the amyotrophic lateral sclerosis spinal cord PMID: 17554784
33. In embryonic stem cells deficient in laminins, due to lamc1 (laminin gamma1) deletion, vascular development and organization were largely unaffected. PMID: 18073332
34. Laminin isoforms containing the gamma3 chain are unable to bind to integrins due to the absence of the glutamic acid residue conserved in the C-terminal regions of the gamma1 and gamma2 chains PMID: 18697739
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亞細(xì)胞定位：

Secreted, extracellular space, extracellular matrix, basement membrane.
組織特異性：

Found in the basement membranes (major component).
數(shù)據(jù)庫(kù)鏈接：

HGNC: 6492

OMIM: 150290

KEGG: hsa:3915

STRING: 9606.ENSP00000258341

UniGene: Hs.609663

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