KLC1 Recombinant Monoclonal Antibody

說明書

中文名稱：

KLC1 Recombinant Monoclonal Antibody
貨號：

CSB-RA189175A0HU
規格：

￥1320
圖片：
Western Blot
Positive WB detected in: A549 whole cell lysate(20μg),HT-29 whole cell lysate(20μg), HEK293T whole cell lysate(20μg), Rat brain tissue lysate(20μg)
All lanes: Kinesin light chain 1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 65 kDa
Observed band size: 69 kDa
Exposure time：2min

IHC image of CSB-RA189175A0HU diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Overlay Peak curve showing SH-SY5Y cells stained with CSB-RA189175A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
其他：

產品詳情
產品評價
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靶點詳情

產品詳情

Uniprot No.：

Q07866
基因名：

KLC1
別名：

Kinesin 2 60/70kDa antibody; Kinesin light chain 1 antibody; Kinesin2 antibody; KLC 1 antibody; KLC antibody; Klc1 antibody; KLC1_HUMAN antibody; KNS 2 antibody; KNS 2A antibody; KNS2 antibody; KNS2A antibody; Medulloblastoma antigen MU MB 2.50 antibody; MGC15245 antibody
反應種屬：

Human, Rat
免疫原：

A synthesized peptide derived from human KLC1
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

1B1
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC, FC

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
FC	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport. The light chain may function in coupling of cargo to the heavy chain or in the modulation of its ATPase activity.
基因功能參考文獻：
1. structural plasticity of the N-terminal capping helix might represent a structural determinant for TPR domain structural versatility in cargo binding PMID: 29036226
2. All binary complexes (KLC1:APP, KLC1:JIP1, and APP:JIP1) contain conformations with favorable binding free energies indicating that KLC1 and JIP1 may take part in APP transport in Alzheimer's disease patients. PMID: 27891669
3. BNIP-2 is a kinesin-1 adapter involved in vesicular transportation in the cytoplasm and that association with cargos depends on interaction of the CRAL-TRIO domain with membrane phosphatidylserine. PMID: 25472445
4. The G allele and GG genotype of KLC1 rs8702 were significantly over-represented among cataract patients, as compared to healthy controls, and were associated with an odds ratio for cataract development. PMID: 25883527
5. Dnm1L interacts with KLC1 through the tetratricopeptide repeat domains. PMID: 25082190
6. Microtubule-bound kinesin-1 and kinesin-3 motor domains were visualized at multiple steps in their ATPase cycles--including their nucleotide-free states--at approximately 7 A resolution using cryo-electron microscopy. PMID: 25209998
7. Studies indicate that FEZ1 (fasciculation and elongation protein zeta 1), SCOCO (short coiled-coil protein) and kinesins (kinesin heavy chain) are involved in biological transport process. PMID: 24116125
8. The expression levels of KLC1 variant E in brain and lymphocytes were significantly higher in Alzheimer's disease patients. PMID: 24497505
9. study provides evidence that the combined effect of three variants within the KLC1 gene may predispose to age-related cataract. PMID: 23776437
10. For the binding of cargos shared by KLC1, we propose a different site located within the groove but not involving N343. PMID: 22470497
11. Data suggest that KLC1 is required for normal neural differentiation, ensuring proper metabolism of AD-associated molecules APP and Tau and for proliferation of neural precursors (NPs). PMID: 22272245
12. Phosphorylation of KLC1 at serine 460 modulates binding and trafficking of calsyntenin-1. PMID: 21385839
13. Results do not convincingly support kinesin light chain 1 (KLC1) as a major susceptibility gene in any of the studied diseases, although there is a small effect of KLC1 in relation to cataract. PMID: 19911314
14. The major binding site for kinesin light chain in kinesin heavy chain has been mapped to residues 789-813 at the C-terminal end of the heavy chain stalk domain. PMID: 12475239
15. For a single nucleotide polymorphism (G58836C in intron 13) in the kinesin light-chain 1 (KNS2) gene, the association between Alzheimer's disease and the C allele is found to be significant. PMID: 15364413
16. KNS2 gene may play a role during early stages of Alzheimer's disease pathogenesis. PMID: 17611642
17. KLC1 gene may be a novel susceptibility gene for age-related cataract. PMID: 17653041
18. microtubule-dependent functions of von Hippel-Lindau tumour suppressor are influenced by kinesin-2 PMID: 17825299
19. The present finding supports the involvement of the cytoskeleton and KNS2 in the development of vascular white matter damage, thereby opening up novel fields in the research into leukariois. PMID: 17977659
20. The KLC1 56836CC variant exerts a significant protective effect on the occurrence of multiple sclerosis. PMID: 17999208
21. The rs8702 variant of the kinesin light chain 1 genotype proved to exert strong amplifying effects on the occurrence and severity of leukoaraiosis in patients with long-lasting poorly controlled severe hypertension PMID: 19046961
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亞細胞定位：

Cell projection, growth cone. Cytoplasmic vesicle. Cytoplasm, cytoskeleton.
蛋白家族：

Kinesin light chain family
組織特異性：

Found in a variety of tissues. Mostly abundant in brain and spine.
數據庫鏈接：

HGNC: 6387

OMIM: 600025

KEGG: hsa:3831

STRING: 9606.ENSP00000414982

UniGene: Hs.20107

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