H2AFY Recombinant Monoclonal Antibody

說明書

中文名稱：

H2AFY Recombinant Monoclonal Antibody
貨號：

CSB-RA135386A0HU
規格：

￥1320
圖片：
Western Blot
Positive WB detected in: Hela whole cell lysate(30μg), HepG2 whole cell lysate(30μg), THP-1 whole cell lysate(30μg), SH-SY5Y whole cell lysate(30μg), MCF-7 whole cell lysate(30μg), COLO-205 whole cell lysate(30μg), HEK293 whole cell lysate(30μg), Mouse brain tissue lysate(30μg), Rat brain tissue lysate(30μg)
All lanes: Histone mH2A1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 40 kDa
Observed band size: 40 kDa
Exposure time：10s

IHC image of CSB-RA135386A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

IHC image of CSB-RA135386A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Immunofluorescence staining of A431 cell with CSB-RA135386A0HU at 1:50 , counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Overlay Peak curve showing THP-1 cells stained with CSB-RA135386A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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靶點詳情

產品詳情

Uniprot No.：

O75367
基因名：

H2AFY
別名：

Core histone macro h2a.1 antibody; Core histone macro-H2A.1 antibody; H2A histone family member Y antibody; H2A.y antibody; H2A/y antibody; H2AF12M antibody; H2AFJ antibody; H2afy antibody; H2AY_HUMAN antibody; Histone H2A.Y antibody; Histone macroH2A1 antibody; Histone macroH2A1.1 antibody; Histone macroH2A1.2 antibody; Macroh2a1 antibody; MACROH2A1.1 antibody; MacroH2A1.2 antibody; Medulloblastoma antigen MU MB 50.205 antibody; Medulloblastoma antigen MU-MB-50.205 antibody; mH2a antibody; mH2A1 antibody
反應種屬：

Human, Mouse, Rat
免疫原：

A synthesized peptide from human H2AFY protein
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

17G2
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC, IF, FC

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Involved in stable X chromosome inactivation. Inhibits the binding of transcription factors, including NF-kappa-B, and interferes with the activity of remodeling SWI/SNF complexes. Inhibits histone acetylation by EP300 and recruits class I HDACs, which induces a hypoacetylated state of chromatin.; Binds ADP-ribose and O-acetyl-ADP-ribose, and may be involved in ADP-ribose-mediated chromatin modulation. Increases the expression of genes involved in redox metabolism, including SOD3.; Represses SOD3 gene expression.
基因功能參考文獻：
1. This article points to a splicing-regulated, proto-oncogenic role for the macroH2A1.2 variant and suggests manipulation of macroH2A1.2 expression as a potential therapeutic means to interfere with tumorigenesis. PMID: 29249653
2. The Skp2-mH2A1-CDK8 axis has a critical role in breast cancer development via dysregulation of the G2/M transition, polyploidy, cell growth dysregulation, and loss of tumor suppression. PMID: 25818643
3. We show that composite nucleosomes containing mH2A and NRF-1 are stably positioned on gene regulatory regions and can buffer transcriptional noise associated with antiviral responses PMID: 25959814
4. High MacroH2A1 expression is associated with epigenetic markers for activation of lipogenic genes in fat-induced steatosis. PMID: 25526730
5. macroH2A1.1 expression correlates with poor survival of triple-negative breast cancer patients PMID: 24911873
6. MacroH2A1.1 and PARP-1 cooperate to regulate transcription by promoting CBP-mediated H2B acetylation. PMID: 25306110
7. MacroH2A1 specifically recruits PELP1 to the promoters of macroH2A1 target genes, but macroH2A1 occupancy occurs independent of PELP1. This recruitment allows macroH2A1 and PELP1 to cooperatively regulate gene expression outcomes. PMID: 24752897
8. The results demonstrate that macroH2A1 is a new factor involved in the regulation of rDNA transcription. PMID: 24071584
9. macro histone variants (macroH2A) are expressed at low levels in stem cells and are up-regulated during differentiation PMID: 23595991
10. Both macroH2A1 isoforms may play a role in hepatocellular carcinoma pathogenesis and may be considered as novel diagnostic markers for human hepatocellular carcinoma. PMID: 23372727
11. MacroH2A1 splicing isoforms differentially regulate the transcription of a set of genes involved in redox metabolism. PMID: 23022728
12. The histone variant macroH2A1.1 is recruited to DNA double-strand breaks through a mechanism involving PARP1. PMID: 23031826
13. Within the macro domain of mH2A1.2, a trinucleotide insertion (-EIS-) sequence not found in mH2A1.1 was essential for the interaction between HER-2 and mH2A1.2 as well as mH2A1.2-induced HER-2 expression and cell proliferation. PMID: 22589551
14. ATRX (alpha-thalassemia/MR, X-linked) is a novel macroH2A-interacting protein PMID: 22391447
15. H2AFY is specifically overexpressed in the blood and frontal cortex of patients with Huntington disease compared with controls PMID: 21969577
16. macroH2A can play either a positive or negative role in transcriptional regulation in a context-dependent manner. Additionally, macroH2A has been linked to the control of the cell cycle and cell proliferation. PMID: 20543561
17. significant inverse correlation between mH2A and CDK8 expression levels exists in melanoma patient samples PMID: 21179167
18. distributed during the maintainance phase of x inactivation throughout cell cycle PMID: 12082075
19. MACROH2A1 deposition is regulated by the CULLIN3/SPOP ligase complex and is actively involved in stable X inactivation. PMID: 15897469
20. A specific interaction between mH2A1.1 and PARP-1 was demonstrated and found to be associated with inactivation of PARP-1 enzymatic activity. PMID: 17158748
21. multiple short sequences dispersed along the macroH2A1 histone domain individually supported enrichment on the inactive X chromosome when introduced into H2A PMID: 17570398
22. A phosphorylation site, S137ph, was identified in mH2A1;S137ph is enriched in mitosis. PMID: 18227505
23. expression of histone macroH2A1.1 and macroH2A2 predicts lung cancer recurrence. PMID: 19648962
24. show that distinct macrodomains, including those of histone macroH2A1.1, are recruited to sites of PARP1 activation induced by laser-generated DNA damage. PMID: 19680243
25. an unexpected role for macroH2A1 in the escape from heterochromatin-associated silencing and the enhancement of autosomal gene transcription PMID: 20008927
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亞細胞定位：

Nucleus. Chromosome.
組織特異性：

Widely expressed.
數據庫鏈接：

HGNC: 4740

OMIM: 610054

KEGG: hsa:9555

STRING: 9606.ENSP00000423563

UniGene: Hs.420272

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