FTH1 Recombinant Monoclonal Antibody

說明書

中文名稱：

FTH1重組抗體
貨號：

CSB-RA009030MA1HU
規格：

￥1320
圖片：
Western Blot
Positive WB detected in: U251 whole cell lysate,JK whole cell lysate
All lanes: FTH1 antibody at 1:500
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 22 kDa
Observed band size: 22 kDa

IHC image of CSB-RA009030MA1HU diluted at 1:50 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.

IHC image of CSB-RA009030MA1HU diluted at 1:50 and staining in paraffin-embedded human rectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.

Overlay Peak curve showing 293 cells stained with CSB-RA009030MA1HU (red line) at 1:50. The cells were fixed in 4% formaldehyde (15min) and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1μg/1*10⁶cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1μg/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品描述：

CSB-RA009030MA1HU FTH1重組單克隆抗體是針對鐵蛋白重鏈（Ferritin Heavy Chain）研發的高特異性檢測工具，適用于多種實驗場景。該靶蛋白作為鐵代謝關鍵調控因子，不僅參與細胞內鐵離子的存儲與釋放，還在抗氧化應激、細胞周期調控及炎癥反應中發揮重要作用，是研究鐵穩態失衡相關疾病機制的重要分子。經嚴格驗證，本抗體在Western Blot中可精準識別天然及重組FTH1蛋白，呈現單一清晰條帶；免疫組化實驗顯示其在多種組織樣本中具有優異的定位特異性，能準確反映靶蛋白的亞細胞分布特征；流式細胞分析則證實其適用于細胞表面及胞內抗原的定量檢測。推薦使用稀釋度為WB 1:1000-5000、IHC 1:20-200、FC 1:20-200，可滿足鐵代謝通路研究、氧化損傷模型分析、腫瘤微環境探索等科研需求，為體外實驗、病理機制解析及藥物靶點篩選提供可靠支持。
Uniprot No.：

P02794
基因名：

FTH1
別名：

Apoferritin antibody; Cell proliferation inducing gene 15 protein antibody; Cell proliferation-inducing gene 15 protein antibody; F HC antibody; Ferritin H subunit antibody; Ferritin heavy chain antibody; Ferritin heavy polypeptide 1 antibody; FHC antibody; FRIH antibody; FRIH_HUMAN antibody; FTH 1 antibody; FTH antibody; FTH1 antibody; FTH1 protein antibody; FTHL 6 antibody; FTHL6 antibody; Iron overload autosomal dominant antibody; MGC104426 antibody; N-terminally processed antibody; OK/SW-cl.84 antibody; PIG 15 antibody; PIG15 antibody; Placenta immunoregulatory factor antibody; PLIF antibody; Proliferation inducing gene 15 protein antibody; Proliferation inducing protein 15 antibody
反應種屬：

Human
免疫原：

Recombinant Human FTH1 protein
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Mouse IgG
純化方式：

Affinity-chromatography
克隆號：

18C10
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC, FC

推薦稀釋比：

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:20-1:200
FC	1:20-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney.
基因功能參考文獻：
1. Human oligodendrocytes undergo apoptosis when exposed to Sema4A and take up H-ferritin for meeting iron requirements and that these functions are mediated via the Tim-1 receptor. H-ferritin can block Sema4A-mediated cytotoxicity. Sema4A is detectable in the CSF of multiple sclerosis patients and HIV-seropositive persons and can induce oligodendrocyte cell death. PMID: 29457657
2. High serum ferritin (SF) and the overall survival of allogeneic hematopoietic SF was only significantly associated with prolonged RBC transfusion dependence post-transplantation. PMID: 29046127
3. the role of FTH1 in the FIH control of HIF-1 activity, is reported. PMID: 29580991
4. Study demonstrate that the amounts of ferritin heavy subunit are inversely correlated, in K562 cells, to H19/miR-675 levels, and that this phenomenon may be largely attributed to the increase in ROS production induced by the FHC silencing. PMID: 29544765
5. Findings indicate that induction of EMT, increased migration and survival depend, in MCF-7 and H460 cells, on the release of FHC control on two pathways, namely the iron/ROS metabolism and CXCR4/CXCL12 axis. PMID: 28774348
6. ferritin heavy chain has a role in ovarian cancer stem cell expansion and epithelial to mesenchymal transition PMID: 27566559
7. Report in vivo magnetic resonance imaging of xenografted neuroblastoma tumors using FTH1 reporter gene expression controlled by a tet-on switch. PMID: 27732930
8. The H-ferritin could bind up to 24 NCOA4(383-522) fragments forming highly stable and insoluble complexes. The binding was partially inhibited only by Fe(II) among the various divalent metal ions analyzed. The iron-dependent, highly-specific formation of the remarkably stable H-ferritin-NCOA4 complex shown in this work may be important for the characterization of the mechanism of ferritinophagy. PMID: 28754384
9. Recombinant human H-chain ferritin nanocages were characterized by transmission electron microscopy and dynamic light scattering. These results indicate that H-chain ferritin is able to self-assemble into nanocages with a narrow size distribution. PMID: 26853188
10. Among myelodysplastic syndromes patients only, CD163 + macrophage density and HO1 and H-ferritin expression by CD163 + macrophages increased in tandem with marrow iron. High HO1 was significantly associated with shorter overall survival. PMID: 26758041
11. Low levels of FTH-positive tumor cells and microglia/macrophages were associated with poor survival in anaplastic astrocytomas, while high amounts of FTL-positive microglia/macrophages had a negative prognostic value PMID: 28837569
12. there is no significant relationship between serum ferritin concentrations and depressive symptoms among Chinese adults. PMID: 27611581
13. Data suggest that, in homopolymeric H-subunit ferritin (HuHF), iron oxidation proceeds with a 2:1 Fe(II):O(2) stoichiometry at iron level of 2 Fe(II) atoms/H-subunit with generation of H2O2; L-subunit-rich heteropolymeric ferritin also facilitates iron oxidation at the ferroxidase center and additionally promotes oxidation at the mineral surface once iron-binding capacity has been exceeded. PMID: 28636371
14. Elevated serum ferritin levels are associated with hematologic malignancies. PMID: 25124580
15. Ferritin light chain and ferritin heavy chain are required for the neural differentiation of bone marrow-derived mesenchymal stem cells under extremely low-frequency electromagnetic field. PMID: 26602884
16. Human FTH1 is a general pro-survival sequence. PMID: 26886577
17. H-ferritin tissue expression and the number of CD68+/H-ferritin+ cells were increased in the lymph nodes of adult-onset Still's disease patients, and these results correlated significantly with disease severity PMID: 26540556
18. Stopped-flow kinetics assays revealed that human H ferritin has different levels of activity compared with its R. catesbeiana counterpart PMID: 26327381
19. Studies indicate that the the best characterized cytosolic ferritins in mammals are encoded by two genes, FTH and FTL, with four exons and similar structures. PMID: 26518749
20. high cytoplasmic FTH1 was associated with favorable prognosis whereas nuclear FTH1 staining was associated with adverse prognosis in triple negative breast cancer PMID: 24742827
21. The goals of this study were to evaluate the multilineage differentiation ability of human mesenchymal stem cells expressing an MRI reporter, human ferritin heavy chain (FTH). PMID: 25448225
22. Data suggest that iron-independent effects of ferritin in myeloma should be prospectively investigated in preclinical and clinical studies. PMID: 24512320
23. Significant differences were seen in the levels of serum iron and hepcidin but not ferritin between the -582A>G hepcidin polymorphism genotypes. PMID: 25134646
24. High ferritin expression is associated with malignant phenotype of breast cancer. PMID: 25265351
25. High serum ferritin levels are associated with pancreatic cancer. PMID: 24446242
26. FTH provides metabolic adaptation to tissue Fe overload. [Review] PMID: 24124891
27. Tumor necrosis factor-alpha attenuates starvation-induced apoptosis through upregulation of ferritin heavy chain in hepatocellular carcinoma cells. PMID: 24066693
28. Elevated serum ferritin level is associated with the incident type 2 diabetes in healthy Korean men. PMID: 24098686
29. Children with autism spectrum disorders have significantly lower ferritin levels compared with controls and higher levels of sleep fragmentation and periodic limb movements. PMID: 24053984
30. Data indicate that serum highest TNF-alpha and IL-6 and higher ferritin are among those with intense steatosis. PMID: 23892696
31. Traumatic brain injury upregulates ferritin-heavy-chain in pericontusional cortex. PMID: 23079850
32. Increased levels of serum ferritin are adversely associated with cardiac function following myocardial infarction treated with percutaneous coronary intervention. PMID: 23079089
33. In normal bone marrow, ferritin H antibody stained early erythroid precursors and macrophages. In leukemic bone marrow, ferritin H was selectively expressed in erythroid blasts (M6), whereas all other blasts were negative. PMID: 23611361
34. The present study provides information about the metal binding sites of human H-chain ferritin and the pathway of ferrous ions (Fe2+) from the cytoplasm to the interior of the ferritin nanocage. PMID: 23344859
35. Serum ferritin levels in infectious diseases was lower than those in hematologic diseases. PMID: 23324584
36. Data indicate that ferritin levels and hyperuricemia are two independently significant factors associated with alanine aminotransferase (ALT) elevation among obese adolescents. PMID: 23119080
37. Increased levels of ferritin and decreased total antioxidant status indicated increasing inflammation in chronic hepatitis C. PMID: 22760008
38. H-ferritin overexpression promotes radiation-induced leukemia/lymphoma. PMID: 22843505
39. The hepcidin:ferritin ratio is reduced in relation to increasing fibrosis in chronic liver disease. PMID: 22676252
40. To study whether the human ferritin heavy chain (FTH1) can be expressed in Hansenula polymorpha, we integrated an expression cassette for FTH1, analyzed the protein expression, and also designed a FTH1-PTH fusion protein. PMID: 22212821
41. Induction of ferritin heavy chain expression results in severe inhibition of apoB-100 secretion from hepatoma cells during HCV infection. PMID: 22443280
42. Survivin inhibition by an interacting recombinant peptide, derived from the human ferritin heavy chain, impedes tumor cell growth PMID: 22426960
43. Elevated serum ferrin in infants is associated with congenital hypothyroidism. PMID: 22768652
44. Data provide useful insights into biomineralization of ferritin under in vivo fever conditions as well as in biomimetic synthesis of nanomaterials using ferritin. PMID: 22020807
45. FTH1 can inhibit the activation of the JNK signaling pathway and can bind to Daxx, inhibiting Daxx-mediated apoptosis. PMID: 21573799
46. H ferritin silenced human metastatic melanoma cells are characterized by a decreased growth activity, a reduced invasiveness, and a reduced cell adhesion capability. PMID: 22043922
47. There was a positive correlation between reactive oxygen species levels and serum ferritin levels in myelodysplastic syndrome patients PMID: 22117997
48. results showed the association of secreted IL-10, FHC and iron homoeostasis in Chlamydia trachomatis-infected HeLa-229 cells PMID: 21413929
49. FTH1 is a functional target gene of the BACH1 transcription factor according to ChIP-seq and knockdown analysis in HEK 293 cells. PMID: 21555518
50. Results illustrate how silencing H-ferritin can effectively sensitize tumors to chemotherapy. PMID: 21385903
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相關疾病：

Hemochromatosis 5 (HFE5)
蛋白家族：

Ferritin family
組織特異性：

Expressed in the liver.
數據庫鏈接：

HGNC: 3976

OMIM: 134770

KEGG: hsa:2495

STRING: 9606.ENSP00000273550

UniGene: Hs.524910

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