FIS1 Recombinant Monoclonal Antibody
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中文名稱：FIS1重組抗體
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貨號：CSB-RA334524A0HU
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規格：￥1320
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圖片：
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Western Blot
Positive WB detected in: HepG2 whole cell lysate, PC3 whole cell lysate, MCF-7 whole cell lysate, Hela whole cell lysate, U251 whole cell lysate, A549 whole cell lysate
All lanes: FIS1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 17 kDa
Observed band size: 17-22 kDa -
IHC image of CSB-RA334524A0HU diluted at 1:100 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. -
IHC image of CSB-RA334524A0HU diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
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其他：
產品詳情
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產品描述：CSB-RA334524A0HU FIS1重組單克隆抗體是針對線粒體分裂蛋白FIS1研發的高特異性檢測工具。該抗體識別的FIS1蛋白是調控線粒體動態平衡的關鍵因子，通過參與線粒體膜的分裂過程影響細胞能量代謝及凋亡信號通路。經多種技術平臺驗證，本產品在Western Blot（WB）中可呈現清晰的特異性條帶，推薦工作濃度為1:500-1:2000；在免疫組化（IHC）實驗中能有效定位組織樣本中線粒體相關信號，推薦使用濃度1:50-1:200；同時適用于ELISA檢測體系。驗證數據顯示其在不同物種來源的樣本中均表現出良好的交叉反應性，且批次間穩定性優異。該抗體特別適用于線粒體形態學研究、細胞代謝調控機制探索以及退行性疾病模型中細胞器功能異常的檢測，為細胞生物學、分子病理學等基礎研究領域提供可靠工具。通過重組單克隆技術制備，確?？贵w具備高度均一性和可重復性，適用于需要精準檢測線粒體分裂相關蛋白表達的科研實驗。
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Uniprot No.：
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基因名：
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別名：Mitochondrial fission 1 protein (FIS1 homolog) (hFis1) (Tetratricopeptide repeat protein 11) (TPR repeat protein 11), FIS1, TTC11
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反應種屬：Human
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免疫原：A synthesized peptide derived from human FIS1
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免疫原種屬：Homo sapiens (Human)
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標記方式：Non-conjugated
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克隆類型：Monoclonal
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抗體亞型：Rabbit IgG
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純化方式：Affinity-chromatography
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克隆號：27B4
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
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產品提供形式：Liquid
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應用范圍：ELISA, WB, IHC
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推薦稀釋比：
Application Recommended Dilution WB 1:500-1:2000 IHC 1:50-1:200 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產品評價
相關產品
靶點詳情
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功能：Involved in the fragmentation of the mitochondrial network and its perinuclear clustering. Plays a minor role in the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface and mitochondrial fission. Can induce cytochrome c release from the mitochondrion to the cytosol, ultimately leading to apoptosis. Also mediates peroxisomal fission.
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基因功能參考文獻：
- Fis1 depletion in osteoarthritis impairs chondrocyte survival and peroxisomal and lysosomal function. PMID: 27497958
- Results show that Fis1 is significantly overexpressed in malignant oncocytomas. PMID: 25822260
- Study revealed that a novel mitochondrial fission pathway composed of miR-483-5p and FIS1 regulates cisplatin sensitivity. PMID: 25843291
- Overexpression of FoxM1 and Plk1 in Fis1-depleted cells restores mitotic entry. PMID: 23907611
- Fis1 expression is significantly increased in spinal cord in a transgenic mouse model of amyotrophic lateral sclerosis. PMID: 23713734
- Fis1 acts as a mitochondrial recruitment factor for TBC1D15 that is involved in regulation of mitochondrial morphology. PMID: 23077178
- PEX11 proteins attract both Mff and human Fis1 (hFis1) to their site of action. PMID: 22595523
- Fis1 and dynamin-related protein (Drp)1 levels are remarkably increased in peripheral blood lymphocytes of Alzheimer's disease patients. PMID: 22340708
- Fis1 activity depends on its ability to interconvert between monomer and dimer species PMID: 22789569
- The mitochondrial fission protein Fission 1 homologue (Fis1) conveys an apoptosis signal from the mitochondria to the ER by interacting with Bap31 at the ER and facilitating its cleavage into the pro-apoptotic p20Bap31. PMID: 21183955
- we observed interactions between Drp1 and Fis1 in mitochondrial fragmentation induced by increasing mitochondrial calcium PMID: 20428767
- hFis1 can bind to multiple amino acid sequences selectively, and the tetratricopeptide repeat constitutes the main binding region of hFis1. PMID: 19864424
- hFis1 is part of the mammalian fission machinery and regulation of the fission processes might be involved in apoptotic mechanisms PMID: 12783892
- regulates mitochondrial fission in mammalian cells through an interaction with the dynamin-like protein DLP1 PMID: 12861026
- Cytosolic domain of fis1 adopts a tetratricopeptide repeat domain fold. PMID: 14705031
- the level of Fis1 at the mitochondrial surface influences mitochondrial fission events and hence overall mitochondrial morphology within the cell PMID: 14996942
- hFis1 induces mitochondrial fragmentation and perinuclear clustering in a state of calcium homeostasis PMID: 15024001
- Fis1 together with dynamin-related protein (Drp1) antogonizes Bcl-2. PMID: 16010987
- These findings provide the first evidence for a role of Fis1 in peroxisomal fission and suggest that the fission machinery of mitochondria and peroxisomes shares common components. PMID: 16107562
- TPR region of hFis1 participates in the interaction with DLP1 or DLP1-containing complex and that the first helix (alpha1) of hFis1 is required for mitochondrial fission presumably by regulating DLP1-hFis1 interaction. PMID: 16118244
- Direct induction of mitochondrial elongation by blocking mitochondrial fission process with Fis1-DeltaTM or Drp1-K38A was sufficient to develop senescent phenotypes with increased Reactive oxygen species production. PMID: 16883569
- hFis1 is a bifunctional protein that independently regulates mitochondrial fragmentation and endoplasmic reticulum-mediated apoptosis. PMID: 16914522
- Fis1 plays important roles in peroxisome division and maintenance of peroxisome morphology in mammalian cells, possibly in a concerted manner with Pex11pbeta and DLP1. PMID: 17408615
- hFis1 and OPA1 modulate cellular senescence PMID: 17545159
- the concave surface of the Fis1 tetratricopeptide repeat-like domain is evolutionarily conserved to bind the dynamin-like GTPase Dnm1 and not Mdv1 as previously predicted PMID: 17884824
- Changes correlate with mitochondrial dysfunction rather than with fragmentation, as substantiated by Fis1 mutants with different effects on organelle shape and function PMID: 18515060
- targeting of hFis1 to peroxisomes and mitochondria are independent events and support a direct, Pex19p-dependent targeting of peroxisomal tail-anchored proteins. PMID: 18782765
- Fis1 and Mfn1 activities influence mitochondrial signal generation thereby insulin exocytosis. PMID: 18832378
- oligomerization of hFis1 in the mitochondrial outer membrane plays a role in mitochondrial fission, potentially through participating in fission factor recruitment. PMID: 18845145
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亞細胞定位：Mitochondrion outer membrane; Single-pass membrane protein. Peroxisome membrane; Single-pass membrane protein.
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蛋白家族：FIS1 family
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數據庫鏈接：
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