ELMO1 Recombinant Monoclonal Antibody

說明書

中文名稱：

ELMO1 Recombinant Monoclonal Antibody
貨號：

CSB-RA185006A0HU
規格：

￥1320
圖片：
Western Blot
, Positive WB detected in: A549 whole cell lysate(30μg), Jurkat whole cell lysate(30μg), Raji whole cell lysate(30μg), SH-SY5Y whole cell lysate(30μg), HEK293 whole cell lysate(30μg), THP-1 whole cell lysate(30μg), Mouse brain tissue lysate(30μg), Rat brain tissue lysate(30μg)
All lanes: ELMO1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 84 kDa
Observed band size: 84 kDa
Exposure time：10s

IHC image of CSB-RA185006A0HU diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

IHC image of CSB-RA185006A0HU diluted at 1:100 and staining in paraffin-embedded human stomach tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
其他：

產品詳情
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靶點詳情

產品詳情

Uniprot No.：

Q92556
基因名：

ELMO1
別名：

CED 12 antibody; Ced 12 homolog 1 antibody; Ced 12 homolog antibody; CED-12 antibody; CED12 antibody; Ced12 homolog 1 antibody; Ced12 homolog antibody; ELMO 1 antibody; ELMO-1 antibody; Elmo1 antibody; ELMO1_HUMAN antibody; Engulfment and cell motility 1 antibody; Engulfment and cell motility protein 1 antibody; KIAA0281 antibody; MGC126406 antibody; Protein ced-12 homolog antibody
反應種屬：

Human, Mouse, Rat
免疫原：

A synthesized peptide from human ELMO1 protein
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

8H7
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in association with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1.
基因功能參考文獻：
1. ELMO1 genetic variation is associated with with type 2 diabetes. PMID: 28752301
2. This study revealed the association of the SNP rs1345365 of the ELMO1 gene in a Mexican population PMID: 29938964
3. A significant association of the SLC12A3 rs11643718 and ELMO1 rs741301 (Single nucleotide Polymorphism) SNPs with diabetic nephropathy in south Indians. PMID: 27699784
4. Cdc27 is a novel binding partner of Elmo1.Cdc27-Elmo1 has a cellular role independent from the Elmo-Dock1-Rac signal module. PMID: 26882976
5. ELMO1 is expressed in rheumatoid arthritis synovium, promotes cell migration and invasion, and regulates Rac1 activity, thereby mediating rheumatoid arthritis pathogenicity. PMID: 25901943
6. Src-mediated Y724 phosphorylation in ELMO1 plays a critical role for cell spreading via activation of Rac1, leading to promotion of cell migration. PMID: 26205662
7. high ELMO1 expression is an independent negative prognostic factor in normal karyotype (NK) acute myeloid leukemia. PMID: 25360637
8. For ELMO1 (+9170 G>A), the GG genotype frequency was higher in the diabetic versus control group, but there were no differences between diabetic patients with and without nephropathies. PMID: 24433479
9. Thus, Elmo1 and Dock180 facilitate blood vessel formation by stabilization of the endothelium during angiogenesis. PMID: 25586182
10. There is a low frequency rate of the ancestral genotype for the ELMO1 polymorphism rs1345365 in mestizos from the western and southeastern regions of Mexico. PMID: 25167351
11. High ELMO1 expression is associated with serous ovarian cancer. PMID: 24819662
12. the present study characterized a novel Nck-1-ELMO1 interaction and defined a new role for Nck-1 in regulating Rac1 activity. PMID: 24928514
13. findings reveal a previously unknown, nonredundant role for Elmo1 in controlling Dock2 levels and Dock2-dependent T cell migration in primary lymphocytes. PMID: 24821968
14. genetic association study in population in China: Data suggest that 2 SNPs in ELMO1 (rs741301; rs10951509) are associated with diabetic nephropathy in Chinese subjects with type 2 diabetes. PMID: 22842811
15. ELMO1 mutations are associated with esophageal adenocarcinoma. PMID: 23525077
16. Analysis of SNP databases of Japanese patuients with diabetic nephropathy revealed ELMO1 as a gene related to the above-cited diabetic complication. PMID: 23156397
17. findings suggest that clearance of apoptotic cells in living vertebrates is accomplished by the combined actions of apoptotic cell migration and elmo1-dependent macrophage engulfment PMID: 22503503
18. Over-expression of NELL1 is associated with alveolar rhabdomyosarcoma. PMID: 22415709
19. The C-terminal Pro-rich tail of ELMO1 winds around the Src-homology 3 domain of DOCK2 to form an intermolecular 5-helix bundle. The entire regions of both DOCK2 a& ELMO1 assemble to create a rigid structure required for the DOCK2 & ELMO1 binding. PMID: 22331897
20. We sequenced 17.4 kb of ELMO1 and identified 19 variants. PMID: 20826100
21. The protein-protein interaction between ELMO1 and COX-2 increased the cyclooxygenase activity of COX-2 and, correspondingly, fibronectin expression.(ELMO1 protein, human) PMID: 20732417
22. findings demonstrate an in vivo role for ELMO1-dependent clearance in the testes, with implications for spermatogenesis PMID: 20958313
23. Dock180 ELMO complex functions as an unconventional two-part exchange factor for Rac. PMID: 12134158
24. the association of DOCK2 with ELMO1 is critical for DOCK2-mediated Rac activation, thereby suggesting that their association might be a therapeutic target for immunologic disorders caused by lymphocyte infiltration PMID: 12829596
25. Rac activation by the ELMO.Dock180 complex at discrete intracellular locations mediated by the N-terminal 330 amino acids of ELMO1 plays a role in cell migration PMID: 14638695
26. Nef binds the DOCK2-ELMO1 complex to activate rac and inhibit lymphocyte chemotaxis PMID: 14737186
27. while N-terminal SH3 of CrkII promotes assembly between CrkII and DOCK180, the C-terminal SH3 of CrkII regulates the stability and turnover of the DOCK180/ELMO complex PMID: 15700267
28. ELMO binding to the SH3 domain of Dock180 disrupted the SH3:Docker interaction, facilitated Rac access to the Docker domain, and contributed to the GEF activity of the Dock180/ELMO complex. PMID: 15723800
29. These results indicate that ELMO1 is a novel candidate gene that both confers susceptibility to diabetic nephropathy and plays an important role in the development and progression of this disease. PMID: 15793258
30. Src family kinase mediated tyrosine phosphorylation of ELMO1 might represent an important regulatory mechanism that controls signaling through the ELMO1/Crk/Dock180 pathway. PMID: 15952790
31. ARNO and ARF6 coordinate with the Dock180/Elmo complex to promote Rac activation at the leading edge of migrating cells. PMID: 16213822
32. Using pulldown assays, we identified engulfment and cell motility (ELMO) protein as the IpgB1 binding partner. IpgB1 colocalized with ELMO and Dock180 in membrane ruffles induced by Shigella. PMID: 17173036
33. Overexpression of ELMO1 and Dock180, a bipartite Rac1 guanine nucleotide exchange factor is associated with glioma cell invasion PMID: 17671188
34. The DOCK180-ELMO1 interaction is mapped to the N-terminal 200 amino acids of DOCK180, and to the C-terminal 200 amino acids of ELMO1, comprising the ELMO1 PH domain. PMID: 18768751
35. Variants in intron 13 of the ELMO1 gene appear to confer risk for diabetic nephropathy in African Americans. PMID: 19183347
36. Report of genetic associations in ELMO1 with diabetic nephropathy, further establishing its role in the susceptibility of this disease. PMID: 19651817
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亞細胞定位：

Cytoplasm. Cell membrane. Note=Translocation to plasma membrane seems to be mediated by DOCK1 and CRK.
組織特異性：

Widely expressed, with a higher expression in the spleen and placenta.
數據庫鏈接：

HGNC: 16286

OMIM: 606420

KEGG: hsa:9844

STRING: 9606.ENSP00000312185

UniGene: Hs.434989

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