DYNLL1 Recombinant Monoclonal Antibody

說明書

中文名稱：

DYNLL1 Recombinant Monoclonal Antibody
貨號：

CSB-RA158858A0HU
規格：

￥1320
圖片：
Flow cytometric analysis of DYNLL1 expression in HepG2 cells using DYNLL1 antibody. Green, isotype control; red, DYNLL1.

Immunocytochemical staining of HepG2 cells with DYNLL1 antibody. Nuclei were stained blue with DAPI; DYNLL1 was stained magenta with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Immunohistochemistry was performed on paraffin-embedded human breast carcinoma using DYNLL1 antibody. Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.

Western blotting analysis using DYNLL1 antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DYNLL1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.
其他：

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靶點詳情

產品詳情

Uniprot No.：

P63167
基因名：

DYNLL1
別名：

DYNLL1; Dynein Light Chain LC8-Type 1; DLC1; DLC8; PIN; DNCL1; LC8; Protein Inhibitor Of Neuronal Nitric Oxide Synthase; Dynein, Cytoplasmic, Light Polypeptide 1; Dynein Light Chain 1, Cytoplasmic; 8 KDa Dynein Light Chain; DNCLC1; Hdlc1; HDLC1; Cytoplasmic Dynein Light Polypeptide; LC8a
反應種屬：

Human, Mouse, Rat
免疫原：

Recombinant Human DYNLL1 protein
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

10A7
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
產品提供形式：

Liquid
應用范圍：

ELISA, WB, FC, ICC, IHC

推薦稀釋比：

Application	Recommended Dilution
WB	1:1000-1:5000
FC	1:200-1:2000
ICC	1:100-1:1000
IHC	1:100-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Flow cytometry (FC) Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. May play a role in changing or maintaining the spatial distribution of cytoskeletal structures.; Binds and inhibits the catalytic activity of neuronal nitric oxide synthase.; Promotes transactivation functions of ESR1 and plays a role in the nuclear localization of ESR1.; Regulates apoptotic activities of BCL2L11 by sequestering it to microtubules. Upon apoptotic stimuli the BCL2L11-DYNLL1 complex dissociates from cytoplasmic dynein and translocates to mitochondria and sequesters BCL2 thus neutralizing its antiapoptotic activity.
基因功能參考文獻：
1. It has been found that APC2 localizes as distinct clusters along microtubule bundles in dendrites, and that this localization is driven by LC8-binding and two separate microtubule-interacting domains. PMID: 30018294
2. High PIN expression is associated with Dilated Hearts. PMID: 27481317
3. An expanded list of LC8 binding partners revealed the evolutionary plasticity of binding partners despite the highly conserved binding interface. In addition, it also highlighted a novel, conserved function of LC8 in the upstream regulation of the Hippo signaling pathway. PMID: 29240760
4. DLC1 binding to nNOS-calmodulin complex does not affect the electron transport from the reductase to the oxygenase domain. PMID: 26923072
5. NMR-derived secondary chemical shifts and relaxation properties show that the Chica LC8 binding domain is essentially disordered with a dynamically restricted segment in one linker between motifs. PMID: 26652654
6. Studies indicate that dynein light chain LC8 has been termed an intrinsically disordered proteins (IDPs) dimerization 'hub' protein. PMID: 26226419
7. DLC1 binding motif in L is involved in cytoskeleton localization and reorganization, primary transcription regulation by DLC1, and regulation of cellular DLC1 gene expression. PMID: 26157129
8. The dynein light intermediate chain has a Ras-like fold with insertions that distinguish it from Ras and other previously described G proteins. PMID: 25272277
9. Authors demonstrate that the interaction between ebola virus VP35 and dynein LC8 is direct and of high affinity and that binding stabilizes the VP35 N-terminal oligomerization domain and enhances viral RNA synthesis. PMID: 25741013
10. Overall, this study demonstrates the novel interaction between HIV-1 integrase and cellular DYNLL1 proteins and suggests the requirement of this virus-cell interaction for proper uncoating and efficient reverse transcription of HIV-1. PMID: 25568209
11. Structural analysis of LC8 with both Nek9 peptides, together with different biophysical experiments, explains the observed diminished binding affinity of Nek9 to LC8 upon phosphorylation on Ser(944) within the Nek9 sequence PMID: 23482567
12. Dynein forms distinct complexes requiring specific recruiters and activators to promote orderly progression through mitosis. PMID: 23589491
13. Overexpressed human LC8 inhibits mouse osteoclast differentiation by regulating NF-kappaB & MAPK pathways and suppressing RANKL signaling. PMID: 23293355
14. DYNLL1 interacted with a spindle-microtubule-associated adaptor formed by CHICA and HMMR via TQT motifs in CHICA. PMID: 22965910
15. appropriate levels of ternary complex components are critical for dynein-dependent spindle positioning in HeLa cells and C. elegans embryos PMID: 23027904
16. Cytosolic mfGbeta is recruited to dynein by Nudel and transported to the centrosome for rapid sequestration and degradation. PMID: 22430153
17. Study found the secretion of calu-1/2-EGFP required microtubule integrity, and that calu-1/2-EGFP-containing vesicles were transported by the motor proteins Kif5b and cytoplasmic dynein. PMID: 22514732
18. The ASCIZ-DYNLL1 feedback loop represents a novel mechanism for auto-regulation of gene expression, where the gene product directly inhibits the transcriptional activator while bound at its own promoter. PMID: 22167198
19. The study uses thermodynamics and dynamics measurements of LC8 complexes to group LC8 binding partners in two categories: those whose binding is enthalpically driven and those that are entropically favored. PMID: 21621319
20. these results imply a potential cellular interference between DYNLL1 and ATMIN functions. PMID: 21971545
21. results significantly widen the scope of the human interactome around DYNLL and will certainly shed more light on the biological functions and organizing role of DYNLL in the human and other eukaryotic interactomes PMID: 21533121
22. DYNLL/LC8 protein controls signal transduction through the Nek9/Nek6 signaling module by regulating Nek6 binding to Nek9. PMID: 21454704
23. Data demonstrate the direct interaction between DYNLL1 and peptides derived from ASFV p54 and gephyrin interacting sequences. PMID: 21094642
24. effect of glycosylation on protein folding thermodynamics and kinetics PMID: 20936810
25. Data suggest that MTOC-directed movement of lytic granules in NK cells is independent of actin and microtubule reorganization, dependent on dynein motor function, occurs before MTOC polarization, and does not require a commitment to cytotoxicity. PMID: 20444980
26. the thermodynamic and kinetic fine-tuning of binding of various ligands to DYNLL could have physiological relevance in its interaction network. PMID: 20889982
27. Data provide the first trafficking regulatory role for Crmp2 in non-neuronal cells and support a model in which Crmp2 is an important endocytic regulatory protein that links MICAL-L1.EHD1-based vesicular transport to dynein motors. PMID: 20801876
28. Data identify GRINL1A as a membrane-associated DYNLL1 binding partner and suggest that additional DYNLL1-binding partners are present near this glutamate channel homolog. PMID: 20412299
29. the regulation of DLC1 by p21-activated kinase 1 is a novel mechanism by which a signaling kinase might influence macropinocytosis PMID: 15504720
30. LC8 binds to p53-binding protein 1 and mediates DNA damage-induced p53 nuclear accumulation PMID: 15611139
31. PAX6 interacts with HOMER3, DNCL1, and TRIM11. Three C-terminal PAX6 mutations, previously identified in patients with eye malformations, all reduced or abolished the interactions. PMID: 16098226
32. the DLC1-KIBRA interaction is essential for ER transactivation in breast cancer cells PMID: 16684779
33. PIN not only inhibits nitric oxide but also .O(2)(-) production from nitric oxide synthase 1 PMID: 16781079
34. dynein light chain 1 represents a novel anchoring protein for RasGRP3 that may regulate subcellular localization of the exchange factor PMID: 17012239
35. we established for the first time expression of PIN/LC8 in human mast cells PMID: 17169380
36. findings reveal a previously unrecognized regulatory mechanism of DLC1 in which the Ser(88) phosphorylation acts as a molecular switch for the transition of DLC1 from dimer to monomer PMID: 18084006
37. LC8 binds IkappaBalpha in a redox-dependent manner and thereby prevents its phosphorylation by IKK. TRP14 contributes to this inhibitory activity by maintaining LC8 in a reduced state. PMID: 18579519
38. pH dependent unfolding characteristics of DLC8 dimer: Residue level details from NMR. PMID: 18692162
39. The data indicated the presence of intermediates along the equilibrium unfolding transition; the hydrogen exchange data suggested that the DLC8 molecule has differential stability in its various segments. PMID: 18767155
40. Hierarchy in guanidine unfolding of DYNLL1 dimer is reported. PMID: 19032974
41. LC8 facilitates nuclear import of Pak1 and this function is indispensable during vertebrate development PMID: 19557173
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亞細胞定位：

Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton. Nucleus. Mitochondrion.
蛋白家族：

Dynein light chain family
組織特異性：

Ubiquitous. Expressed in testis.
數據庫鏈接：

HGNC: 15476

OMIM: 601562

KEGG: hsa:8655

STRING: 9606.ENSP00000242577

UniGene: Hs.5120

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