CLPX Recombinant Monoclonal Antibody

說明書

中文名稱：

CLPX Recombinant Monoclonal Antibody
貨號：

CSB-RA052595A0HU
規格：

￥1320
圖片：
Flow cytometric analysis of CLPX expression in HepG2 cells using CLPX antibody. Green, isotype control; red, CLPX.

Immunocytochemical staining of HepG2 cells with CLPX antibody. Nuclei were stained blue with DAPI; CLPX was stained magenta with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using CLPX antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CLPX antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. CLPX, caseinolytic mitochondrial matrix peptidase chaperone subunit X.
其他：

產品詳情
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靶點詳情

產品詳情

Uniprot No.：

O76031
基因名：

CLPX
別名：

Caseinolytic Mitochondrial Matrix Peptidase Chaperone Subunit X; ATP-Dependent Clp Protease ATP-Binding Subunit ClpX-Like, Mitochondrial; ClpX (Caseinolytic Protease X, E. Coli) Homolog ; ClpX Caseinolytic Protease X Homolog (E. Coli); Energy-Dependent Regulator Of Proteolysis; ClpX Caseinolytic Peptidase X Homolog; ClpX Caseinolytic Protease X Homolog; EPP2
反應種屬：

Human, Mouse, Rat
免疫原：

Recombinant Human CLPX protein
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

5C7
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
產品提供形式：

Liquid
應用范圍：

ELISA, WB, FC, ICC

推薦稀釋比：

Application	Recommended Dilution
WB	1:1000-1:5000
FC	1:200-1:2000
ICC	1:100-1:1000

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

ATP-dependent specificity component of the Clp protease complex. Hydrolyzes ATP. Targets specific substrates for degradation by the Clp complex. Can perform chaperone functions in the absence of CLPP. Enhances the DNA-binding activity of TFAM and is required for maintaining a normal mitochondrial nucleoid structure. ATP-dependent unfoldase that stimulates the incorporation of the pyridoxal phosphate cofactor into 5-aminolevulinate synthase, thereby activating 5-aminolevulinate (ALA) synthesis, the first step in heme biosynthesis. Important for efficient erythropoiesis through upregulation of heme biosynthesis.
基因功能參考文獻：
1. The mutation in CLPX inactivates its ATPase activity, resulting in coassembly of mutant and WT protomers to form an enzyme with reduced activity. The presence of low-activity CLPX increases the posttranslational stability of ALAS, causing increased ALAS protein and ALA levels, leading to abnormal accumulation of PPIX. PMID: 28874591
2. Data suggest that tumors exploit ClpXP-directed proteostasis to maintain mitochondrial bioenergetics, buffer oxidative stress, and enable metastatic competence. PMID: 27389535
3. results illustrate that ClpX overexpression is a good and simple model to study the underlying mechanisms of the UPRmt in mammalian cells. PMID: 26142927
4. Optical trapping to assay single-molecule ClpXP unfolding and translocation of substrates consisting of domains with varying stabilities and sequences; find that ClpXP unfolds most domains by a single pathway, with kinetics that depend on the native fold and structural stability. PMID: 25083874
5. Walker B mutation in human CLPX exhibits improved interaction with the model unfolded substrate casein and several putative physiological substrates in vitro. PMID: 22710082
6. human ClpX, a novel mtDNA regulator, maintains mtDNA nucleoid distribution through TFAM function as a chaperone rather than as a protease and its involvement in mtDNA segregation. PMID: 22841477
7. hClpX can regulate the appearance of hClpP peptidase activity in mitochondria and might affect the nature of the degradation products released during ATP-dependent proteolytic cycles PMID: 16115876
8. Results reveal that the ssrA tag interacts with different loops that form the top, middle, and lower portions of the central channel of the ClpX hexamer. PMID: 18313382
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亞細胞定位：

Mitochondrion. Mitochondrion matrix, mitochondrion nucleoid.
蛋白家族：

ClpX chaperone family
組織特異性：

Higher expression in skeletal muscle and heart and to a lesser extent in liver, brain, placenta, lung, kidney and pancreas.
數據庫鏈接：

HGNC: 2088

OMIM: 615611

KEGG: hsa:10845

STRING: 9606.ENSP00000300107

UniGene: Hs.113823

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