CLIP1 Recombinant Monoclonal Antibody

說明書

中文名稱：

CLIP1 Recombinant Monoclonal Antibody
貨號：

CSB-RA266046A0HU
規格：

￥1320
圖片：
Flow cytometric analysis of CLIP1 expression in HepG2 cells using CLIP1 antibody. Green, isotype control; red, CLIP1.

Immunocytochemical staining of HepG2 cells with CLIP1 antibody. Nuclei were stained blue with DAPI; CLIP1 was stained magenta with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using CLIP1 antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CLIP1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.
其他：

產品詳情
產品評價
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靶點詳情

產品詳情

Uniprot No.：

P30622
基因名：

CLIP1
別名：

CLP1; Cleavage Factor Polyribonucleotide Kinase Subunit 1; HEAB; Cleavage And Polyadenylation Factor I Subunit 1; Polyribonucleotide 5'-Hydroxyl-Kinase Clp1; Pre-MRNA Cleavage Complex II Protein Clp1; Polyadenylation Factor Clp1; Polynucleotide Kinase Clp1; EC 2.7.1.78; HClp1; CLP1, Cleavage And Polyadenylation Factor I Subunit, Homolog (S. Cerevisiae); Polyribonucleotide 5'-Hydroxyl-Kinase; Homolog Of Yeast CFIA Subunit Clp1p; ATP/GTP-Binding Protein; ATP/GTPbinding Protein; HCLP1
反應種屬：

Human, Rat
免疫原：

Recombinant Human CLIP1 protein
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

11E7
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
產品提供形式：

Liquid
應用范圍：

ELISA, WB, FC, ICC

推薦稀釋比：

Application	Recommended Dilution
WB	1:1000-1:5000
FC	1:200-1:2000
ICC	1:100-1:1000

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Binds to the plus end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Promotes microtubule growth and microtubule bundling. Links cytoplasmic vesicles to microtubules and thereby plays an important role in intracellular vesicle trafficking. Plays a role macropinocytosis and endosome trafficking.
基因功能參考文獻：
1. we report an unexpected finding that CLIP170 negatively regulates TLR4 signaling by the targeted ubiquitination and degradation of TIRAP. Furthermore, we observed that CLIP170 expression is modulated by LPS to maintain the cellular homeostasis. PMID: 29222167
2. ASK1- induced phosphorylation of EB1 not only increases its plus end-tracking ability, but also promotes its recruitment of CLIP170 and p150glued to astral microtubules. PMID: 28039481
3. single-molecule fluorescence microscopy showed that the microtubule plus-end-associated protein CLIP-170 binds tightly to formins to accelerate actin filament elongation. PMID: 27199431
4. We find that LRRK1-mediated phosphorylation of CLIP-170 causes the accumulation of p150(Glued) (also known as DCTN1) a subunit of dynactin, at microtubule plus ends, thereby facilitating the migration of EGFR-containing endosomes. PMID: 25413345
5. herpesvirus particles are absolutely dependent on CLIP-170-mediated capture to initiate transport in primary human cells. PMID: 26504169
6. We show that AMPH-1/BIN1 binds to nesprin and actin, as well as to the microtubule-binding protein CLIP170 in both species. We propose that BIN1 has a direct and evolutionarily conserved role in nuclear positioning, altered in myopathies. PMID: 26506308
7. Restin inhibits epithelial-mesenchymal transition and tumor metastasis by controlling the expression of the tumor metastasis suppressor mir-200a/b via association with p73. PMID: 25972084
8. CLIP-170 tethers kinetochores to microtubule ends against the dynein-mediated poleward force to slide kinetochores along microtubules PMID: 26231764
9. A defect in the CLIP1 gene (CLIP-170) can cause autosomal recessive intellectual disability. PMID: 24569606
10. Data suggest that CLIP-170 acts as a novel recruiter and spatial regulator of PLK1 at kinetochores during early mitosis, promoting K-fiber stability and chromosome alignment for error-free chromosome segregation. PMID: 24777477
11. siRNA-mediated knockdown of the cytoplasmic linker protein compromised the assembly and branching of capillary-like blood vessels and neovascularization in vivo. It was critical for the motility abilities of HUVECs through its actions on cell polarity. PMID: 24530770
12. HDAC6 interacts with cytoplasmic linker protein 170 (CLIP-170) and that these two proteins function together to stimulate the migration of pancreatic cancer cells. PMID: 24474193
13. CLIP-170 phosphorylation by Plk1 regulates proper chromosome alignment PMID: 24451569
14. Depletion of CLIP-170 significantly impaired vascular endothelial tube formation and sprouting in vitro and inhibited breast tumor growth in mice by decreasing tumor vascularization. PMID: 23549612
15. We further demonstrate that this binding was prevented when the C-terminal tyrosine of EB1 was absent in the peptidic probes. PMID: 22543185
16. results suggest that EB1 and ClipCG12 act cooperatively to regulate microtubule dynamics (CLIP-170) PMID: 22424550
17. End-binding proteins interact with the CAP-Gly domains of CLIP-170 and p150(glued). PMID: 22119847
18. These results demonstrate that CLIP-170 mediates paclitaxel sensitivity in breast cancer via a microtubule-dependent mechanism. PMID: 21989536
19. Herein, the authors have identified polo-like kinase 1 (Plk1) and casein kinase 2 (CK2) as two kinases of CLIP-170 and mapped S195 and S1318 as their respective phosphorylation sites. PMID: 20664522
20. LIS1, CLIP-170's key to the dynein/dynactin pathway PMID: 11940666
21. The cytoplasmic linker protein CLIP-170 is a human autoantigen PMID: 11966772
22. Rac1 and Cdc42 capture microtubules through IQGAP1 and CLIP-170. PMID: 12110184
23. expressed at high levels in monocyte-derived dendritic cells and IL-4 + CD40L-activated B cells and is involved in the trafficking of macropinosomes to the cytoskeleton, a crucial step in antigen presentation PMID: 12433698
24. neogenin and restin have roles in proproliferation/survival action on ovarian cancer cells PMID: 12833147
25. The restin gene was amplified from retinoic acid-treated promyelocytic cell line HL-60 by RT-PCR and cloned into a prokaryotic expression vector. PMID: 15862147
26. these data explain observations that CLIP-170 localizes to newly polymerized microtubules in vitro but cannot track microtubule plus-ends in vitro. These observations have implications for the functions of CLIP-170 in regulating microtubule dynamics PMID: 16120651
27. CLIP-170 facilitates the formation of kinetochore-microtubule attachments, possibly through direct capture of microtubules at the kinetochore. PMID: 16362039
28. STAT-1alpha plays an important role in the atRA-induced transcriptional up-regulation of restin PMID: 16574066
29. The function of CLIP-170 in membrane trafficking is not associated with plus-end localization. PMID: 16772339
30. A structural basis for tubulin recognition by CLIP-170 and its autoinhibition is provided. PMID: 17563362
31. Data use the complex formed between the CAP-Gly domain of p150(glued) and the C-terminal zinc knuckle of CLIP170 as a model system to explore the structure-function relationship of CAP-Gly-mediated protein interactions. PMID: 17828277
32. Crystal structures of the tubulin binding domains of XMAP215 (yeast Stu2p and Drosophila Msps), EB1 (yeast Bim1p and human EB1), and CLIP-170 (human), which reveal diverse tubulin binding interfaces, are reported. PMID: 17889670
33. The results suggest that promotion of lamellipodia formation and invasion by HGF or serum requires PI3K-dependent release of IQGAP1 and kinesin from Rac1-CLIP-170 complex. PMID: 18237546
34. CLIP-170 was expressed in differentiated keratinocytes, first at the periphery of the nucleus then with a granular cytoplasmic labeling evocative of lamellar bodies PMID: 18622020
35. Amphiphysin 2/BIN1 participates in the tubulation of traffic intermediates and intracellular organelles first via its intrinsic tubulating potential and second via its ability to bind CLIP-170 and microtubules. PMID: 19004523
36. these results suggest that complexes of dynein, Lis1 and CLIP-170 crosslink and slide microtubules within the spindle, thereby producing an inward force that pulls centrosomes together. PMID: 19020519
37. EB1 is both necessary and sufficient to mediate microtubule plus-end tracking by CLIP-170. PMID: 19126680
38. Cdc2-mediated phosphorylation of CLIP-170 is essential for the normal function of this protein during cell cycle progression PMID: 19687009
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亞細胞定位：

Cytoplasm. Cytoplasm, cytoskeleton. Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, ruffle.
組織特異性：

Detected in dendritic cells (at protein level). Highly expressed in the Reed-Sternberg cells of Hodgkin disease.
數據庫鏈接：

HGNC: 10461

OMIM: 179838

KEGG: hsa:6249

STRING: 9606.ENSP00000303585

UniGene: Hs.524809

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