CLGN Recombinant Monoclonal Antibody

說明書

中文名稱：

CLGN Recombinant Monoclonal Antibody
貨號：

CSB-RA230651A0HU
規格：

￥1320
圖片：
Immunocytochemical staining of Hela cells with Calmegin antibody. Nuclei were stained blue with DAPI; Calmegin was stained magenta with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using calmegin antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with calmegin antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.
其他：

產品詳情

Uniprot No.：

O14967
基因名：

CLGN
別名：

CLGN; Calmegin; Testis Tissue Sperm-Binding Protein Li 79P
反應種屬：

Human
免疫原：

Recombinant Human CLGN protein
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

7A7
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
產品提供形式：

Liquid
應用范圍：

ELISA, WB, ICC

推薦稀釋比：

Application	Recommended Dilution
WB	1:1000-1:5000
ICC	1:100-1:1000

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

功能：

Functions during spermatogenesis as a chaperone for a range of client proteins that are important for sperm adhesion onto the egg zona pellucida and for subsequent penetration of the zona pellucida. Required for normal sperm migration from the uterus into the oviduct. Required for normal male fertility. Binds calcium ions.
基因功能參考文獻：
1. In gene-based analysis CLGN1 represented one of a cluster of genes, which interacted with sodium to influence Blood Pressure. PMID: 27271309
2. Although their similarity in carbohydrate binding specificities is high, there seems to be some differences in the mode of substrate recognition between calmegin and calnexin. PMID: 24769397
3. calmegin expression is regulated by histone deacetylase and CpG methyltransferase in a coordinative way PMID: 16264275
亞細胞定位：

Endoplasmic reticulum membrane; Single-pass type I membrane protein.
蛋白家族：

Calreticulin family
組織特異性：

Detected in testis (at protein level). Detected in testis.
數據庫鏈接：

HGNC: 2060

OMIM: 601858

KEGG: hsa:1047

STRING: 9606.ENSP00000326699

UniGene: Hs.86368

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