CHAF1B Recombinant Monoclonal Antibody

說明書

中文名稱：

CHAF1B Recombinant Monoclonal Antibody
貨號：

CSB-RA932844A0HU
規格：

￥1320
圖片：
Flow cytometric analysis of CHAF1B expression in HT-1080 cells using CHAF1B antibody. Green, isotype control; red, CHAF1B.

Immunocytochemical staining of HT-1080 cells with CHAF1B antibody. Nuclei were stained blue with DAPI;CHAF1B was stained magenta with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using CHAF1B antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CHAF1B antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.
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產品詳情
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靶點詳情

產品詳情

Uniprot No.：

Q13112
基因名：

CHAF1B
別名：

CHAF1B; Chromatin Assembly Factor 1 Subunit B; MPHOSPH7; CAF1P60; CAF1A; MPP7; M-Phase Phosphoprotein 7; CAF-1; CAF1; Human Chromatin Assembly Factor-I P60 Subunit; Chromatin Assembly Factor I P60 Subunit; CAF-I 60 KDa Subunit; CAF-1 Subunit B; CAF-I P60; Chromatin Assembly Factor 1, Subunit B (P60); Chromatin Assembly Factor I, P60 Subunit; CAF-IP60
反應種屬：

Human
免疫原：

Recombinant Human CHAF1B protein
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

7E5
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
產品提供形式：

Liquid
應用范圍：

ELISA, WB, FC, ICC

推薦稀釋比：

Application	Recommended Dilution
WB	1:1000-1:5000
FC	1:200-1:2000
ICC	1:100-1:1000

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer.
基因功能參考文獻：
1. The volumes of the tumors were similar between those injected with the empty vector and control, but were significantly smaller in the knockdown models, suggesting that the knockdown of the CHAF1B gene inhibited tumor growth. H&E staining revealed that tumors were developed in mice in all groups. PMID: 29767268
2. All the evaluated malignant tumors showed CAF-1 p60 overexpression, confirming the emerging role of CAF-1 p60 as a new proliferation and prognostic marker for cancer. PMID: 23109837
3. The levels of the EDNRB, HJURP and p60/CAF-1 proteins were strongly associated with overall survival in high-grade gliomas patients (p<0.001, p<0.001 and p=0.002, respectively), whereas the one of PDLI4 was not (P=0.11). PMID: 24039914
4. Overexpression of CAF-1/p60, on histological and/or cytological samples, characterizes malignant salivary gland tumours with aggressive behaviour PMID: 21109952
5. CAF-1 is a proliferation marker in various malignant tumours with prognostic value in renal, endometrial and cervical carcinomas, which supports the value of CAF-1 as a clinical marker of cancer progression. PMID: 21083601
6. Chromatin Assembly Factor-1/p60 may have a role in progression and metastasis of melanoma PMID: 20178651
7. model is proposed in which the synergism between hAsf1 and CAF-1 for nucleosome formation during DNA repair is achieved through a transient physical interaction allowing histone delivery from Asf1 to CAF-1 [asf1(anti-silencing function 1)] PMID: 11897662
8. These data identify chromatin assembly factor 1 as an essential factor not only for S-phase-specific chromatin assembly but also for proliferating cell viability. PMID: 15024074
9. Downregulated protein level of CAF-1 p60 in adult brain of patients with Down syndrome (DS) in contrast to Alzheimer's disease indicates that it can be considered specific for changes in DS. PMID: 15068244
10. The N- & C-terminal regions of ASF1a and ASF1b determine the different affinities of these two proteins for HIRA, by contacting regions outside the HIRA B domain. CAF-1 p60 also uses B domain-like motifs for binding to ASF1a, thereby competing with HIRA. PMID: 16980972
11. overexpression of CAF-1/p60 characterized prostatic cancers with a worse prognosis PMID: 19309489
12. The histone H3K9 methyltransferase SetDB1 associates with the specific heterochromatin protein 1alpha (HP1alpha)-chromatin assembly factor 1 (CAF1) chaperone complex. PMID: 19498464
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亞細胞定位：

Nucleus. Cytoplasm. Note=DNA replication foci. Cytoplasmic in M phase.
蛋白家族：

WD repeat HIR1 family
數據庫鏈接：

HGNC: 1911

OMIM: 601245

KEGG: hsa:8208

STRING: 9606.ENSP00000315700

UniGene: Hs.75238

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