CAPNS1 Recombinant Monoclonal Antibody

說明書

中文名稱：

CAPNS1 Recombinant Monoclonal Antibody
貨號：

CSB-RA276957A0HU
規格：

￥1320
圖片：
Flow cytometric analysis of Calpain small subunit 1 expression in HepG2 cells using Calpain small subunit 1 antibody. Green, isotype control; red, Calpain small subunit 1.

Immunocytochemical staining of HepG2 cells with Calpain small subunit 1 antibody. Nuclei were stained blue with DAPI; Calpain small subunit 1 was stained magenta with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using calpain small subunit 1 antibody. Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with calpain small subunit 1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.
其他：

產品詳情
產品評價
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靶點詳情

產品詳情

Uniprot No.：

P04632
基因名：

CAPNS1
別名：

CAPNS1; Calpain Small Subunit 1; CDPS; CANPS; CAPN4; CANP; Calcium-Activated Neutral Proteinase Small Subunit; Calcium-Dependent Protease Small Subunit 1; Calpain Regulatory Subunit; CANP Small Subunit; CSS1; 30K; Epididymis Secretory Sperm Binding Protein; Calcium-Dependent Protease, Small Subunit; Calcium-Dependent Protease Small Subunit; Calpain 4, Small Subunit (30K); Calpain, Small Polypeptide; CALPAIN4; CAPNS
反應種屬：

Human, Rat
免疫原：

Recombinant Human CAPNS1 protein
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

2E7
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
產品提供形式：

Liquid
應用范圍：

ELISA, WB, FC, ICC

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:2500
FC	1:200-1:2000
ICC	1:100-1:1000

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Regulatory subunit of the calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
基因功能參考文獻：
1. that Capn4 promotes cell proliferation by increasing MAPK7 expression PMID: 29331389
2. Calpain small subunit 1 (Capn4) overexpression increased the protein level of cleaved talin and and activated the focal adhesion kinase (FAK)/AKT/MAPK signaling in 786-O cells, while Capn4 silencing decreased the protein level of cleaved talin in Caki-1 cells. PMID: 29648579
3. Capn4 and miR-1271 may serve as potential therapeutic targets for the treatment of colorectal cancer. PMID: 29331762
4. MALAT1 and Capn4 were up-regulated while miR-124 expression was down-regulated in nasopharyngeal carcinoma cell lines. PMID: 28857668
5. Capn4 promotes epithelial-mesenchymal transition in human melanoma cells through activation of the Wnt/beta-catenin pathway. PMID: 27878263
6. CAPNS1 depletion is coupled to impairment of breast cancer cell growth. PMID: 26771715
7. High levels of Capn4 expression were associated with lymph node metastasis and large tumor size in non-small cell lung cancer patients.Capn4 promotes non-small cell lung cancer progression via upregulation of matrix metalloproteinase 2. PMID: 25636510
8. HBXIP was able to up-regulate Capn4 at the levels of promoter. PMID: 25304384
9. The overexpression of Capn4 is a novel negative prognostic marker, and Capn4 may be used as a new target in therapeutic strategies for human glioma. PMID: 24628706
10. Capn4 can contribute to hepatocellular carcinoma growth and metastasis PMID: 24962955
11. Capn4 mRNA and protein are increased in human clear cell renal cell carcinoma tissues and higher Capn4 levels are associated with advanced clinical tumour stage and shorter overall survival. PMID: 24514433
12. Capn4 upregulated MMP2 via nuclear factor-kappaB activation, manifested by increased phosphorylation of p65, a subunit of NF-kappaB. PMID: 24703594
13. This study suggests that augmented calpain activity is associated with increased incidence of thrombosis under hypoxic environments. PMID: 24297866
14. Capn4 overexpression was implicated in ICC metastasis/invasion, and Capn4 overexpression may be used as a molecular therapeutic target for intrahepatic cholangiocarcinoma PMID: 23349941
15. The authors conclude that hepatitis B virus X protein upregulate Capn4 through NF-kappaB/p65 to promote migration of hepatoma cells. PMID: 20419804
16. CAPNS1 depletion is coupled to reduced levels of H2AX phosphorylation, not only in Ras(v12) induced BJ-ET fibroblasts, but also in a number of cellular systems upon genotoxic stress PMID: 20107320
17. Genetic variations in LEP, CAPNS1 and ALOX15 show significant association with IL-6 expression. PMID: 19942621
18. calpain-mediated XIAP degradation contributes to initiation of apoptosis in normal neutrophils and dysfunction of this regulatory pathway can lead to pathological neutrophil accumulation. PMID: 12121983
19. Whereas alphaPIX guanine nucleotide exchange factor activity contributes to enhanced formation of cellular protrusions, the GEF-independent association with calpain 4 leads to induction of a yet unknown signaling cascade resulting in cell spreading. PMID: 15611136
20. results reveal that especially NRF-1, along with AP-1 and, to a minor extent, an Sp1 site, is essential for human CAPNS1 promoter activity and gene expression PMID: 18234454
21. c-Myc regulates calpain activity through calpastatin; apoptosis induced by calpain inhibition is dependent on c-Myc, and calpastatin knockdown promotes transformation in c-Myc-negative cells PMID: 18544539
22. RasGAP and Capns1 interaction in oncogenic Ras cells is involved in regulating migration and cell survival. PMID: 18761085
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亞細胞定位：

Cytoplasm. Cell membrane.
數據庫鏈接：

HGNC: 1481

OMIM: 114170

KEGG: hsa:826

STRING: 9606.ENSP00000246533

UniGene: Hs.515371

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