CAD Recombinant Monoclonal Antibody

說明書

中文名稱：

CAD Recombinant Monoclonal Antibody
貨號：

CSB-RA788639A0HU
規格：

￥1320
圖片：
Flow cytometric analysis of CAD expression in HepG2 cells using CAD antibody. Green, isotype control; red, CAD.

Immunocytochemical staining of HepG2 cells with CAD antibody. Nuclei were stained blue with DAPI; CAD was stained magenta with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using CAD antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CAD antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

Uniprot No.：

P27708
基因名：

CAD
別名：

CAD; Carbamoyl-Phosphate Synthetase 2, Aspartate Transcarbamylase, And Dihydroorotase; GATD4; CAD Protein; Multifunctional Protein CAD; CAD Trifunctional Protein; EC 6.3.5.5; EC 2.1.3.2; EC 3.5.2.3; EC 6.3.5; EIEE50; CDG1Z; DEE50
反應種屬：

Human
免疫原：

Recombinant Human CAD protein
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

20B10
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
產品提供形式：

Liquid
應用范圍：

ELISA, WB, FC, ICC

推薦稀釋比：

Application	Recommended Dilution
WB	1:1000-1:5000
FC	1:200-1:2000
ICC	1:100-1:1000

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

This protein is a 'fusion' protein encoding four enzymatic activities of the pyrimidine pathway (GATase, CPSase, ATCase and DHOase).
基因功能參考文獻：
1. CAD dihydroorotase and aspartate transcarbamoylase domains self-assembles into dimers and trimers. PMID: 28552578
2. Detection of the CAD-ALK gene fusion in urine tr-DNA anticipated radiological confirmation of disease progression. Analysis of plasma ctDNA identified ALK kinase mutations that emerged during treatment with the ALK inhibitor entrectinib PMID: 28368455
3. Charge neutralization in the active site of the catalytic trimer of aspartate transcarbamoylase promotes diverse structural changes. PMID: 28833948
4. This study showed that CAD deficiency co-occurrence of anaemia, anisopoikilocytosis, global developmental delay, and seizures. PMID: 28007989
5. Previous study found three metal ions in huDHOase active site; in the present study, the putative third metal binding site in type II enzymes, such as StDHOase and ScDHOase, was created and identified. PMID: 26446564
6. Changes in glycosylation in caused by mutations in CAD. PMID: 25678555
7. These results establish CAD as a downstream effector of Rheb and suggest a possible role of Rheb in regulating de novo pyrimidine nucleotide synthesis PMID: 25422319
8. Recombinant aspartate carbamoyltransferase domain from the CAD enzyme complex forms homotrimers in solution. PMID: 24316846
9. The results obtained indicate that mLST8 bridges between CAD and mTOR, and plays a role in the signaling mechanism where CAD is regulated in the mTOR pathway through the association with mLST8 PMID: 23594158
10. preliminary X-ray diffraction analysis of the dihydroorotase domain of human CAD PMID: 23143245
11. findings show that in prostate tumor cells, CAD fosters androgen receptor translocation into the nucleus and stimulates its transcriptional activity; in radical prostatectomy specimens, CAD expression was not correlated with proliferation markers, but a higher CAD mRNA level was associated with local tumor extension and cancer relapse PMID: 21982950
12. the cad gene is regulated by a nonclassical ERalpha/Sp1-mediated pathway. PMID: 12746293
13. Data show that PRMT5 can be found in association with hSWI/SNF complexes and is involved in regulating the expression of carbamoyl-phosphate synthase-aspartate carbamoyltransferase-dihydroorotase. PMID: 14559996
14. the nuclear import of CAD appears to promote optimal cell growth PMID: 15890648
15. cad gene expression is repressed by hypoxia-inducible factor-1alpha, which represents a functional link between hypoxia and cell-cycle arrest. PMID: 16155188
16. hCPS_DeltaA was not able to fully assume the catalytically competent conformation, with specific activity of CP formation decreased 700-fold. PMID: 18679823
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相關疾病：

Epileptic encephalopathy, early infantile, 50 (EIEE50)
亞細胞定位：

Cytoplasm. Nucleus. Note=Cytosolic and unphosphorylated in resting cells, translocates to the nucleus in response to EGF stimulation, nuclear import promotes optimal cell growth.
蛋白家族：

Metallo-dependent hydrolases superfamily, DHOase family, CAD subfamily
數據庫鏈接：

HGNC: 1424

OMIM: 114010

KEGG: hsa:790

STRING: 9606.ENSP00000264705

UniGene: Hs.377010

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