ARPC2 Recombinant Monoclonal Antibody
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中文名稱：ARPC2 Recombinant Monoclonal Antibody
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貨號：CSB-RA197523A0HU
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規格：￥1320
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圖片：
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Flow cytometric analysis of ARPC2 expression in HT-1080 cells using ARPC2 antibody. Green, isotype control; red, ARPC2. -
Immunocytochemical staining of HT-1080 cells with ARPC2?antibody. Nuclei were stained blue with DAPI; ARPC2 was stained magenta with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm. -
Immunohistochemistry was performed on paraffin-embedded human breast carcinoma using ARPC2 antibody. Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm. -
Western blotting analysis using ARPC2 antibody. Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ARPC2 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.
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其他：
產品詳情
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Uniprot No.：
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基因名：
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別名：Actin Related Protein 2/3 Complex Subunit 2;ARC34;P34-Arc;Actin-Related Protein 2/3 Complex Subunit;Arp2/3 Complex 34 KDa Subunit;Actin Related Protein 2/3 Complex, Subunit 2 (34 KD) ;Actin Related Protein 2/3 Complex, Subunit 2, 34kDa ;Actin Related Protein 2/3 Complex Subunit 2, 34kDa ;Testis Tissue Sperm-Binding Protein Li 53e ;ARP2/3 Protein Complex Subunit 34 ;PNAS-139 ;PRO2446;P34-ARC
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反應種屬：Human, Mouse, Rat
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免疫原：Recombinant Human ARPC2 protein
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免疫原種屬：Homo sapiens (Human)
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標記方式：Non-conjugated
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克隆類型：Monoclonal
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抗體亞型：Rabbit IgG
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純化方式：Affinity-chromatography
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克隆號：18C8
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Rabbit IgG in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
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產品提供形式：Liquid
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應用范圍：ELISA, WB, FC, ICC, IHC
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推薦稀釋比：
Application Recommended Dilution WB 1:1000-1:5000 FC 1:200-1:2000 ICC 1:100-1:1000 IHC 1:100-1:200 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產品評價
相關產品
靶點詳情
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功能：Actin-binding component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility. Seems to contact the mother actin filament. In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA. The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs).
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基因功能參考文獻：
- In the association analysis of 110 gastric cancer tissues, ARPC2 showed significant associations with large tumor size, lymph node invasion, and high tumor stage. In addition, ARPC2-positive patients exhibited lower RFS and OS rates compared with ARPC2-negative patients. We thus identify that ARPC2 plays an aneretic role in human gastric cancer and provided a new target for gastric cancer therapy. PMID: 28694563
- Downregulation of the ARP2/3 complex signaling pathway, a common final pathway for multiple signaling cascades that regulate the actin cytoskeleton, would compromise the structural stability of spines, leading to their loss. In concert with findings from deletion of the ARP2/3 complex in mice, these findings support the idea that spine deficits in the DLPFC may contribute to subcortical hyperdopaminergia in schizophrenia. PMID: 27523502
- Recent reports now demonstrate a novel aspect of the ARP2/3 complex and the nucleating-promoting factors in the maintenance of endothelial barrier function and junction remodeling of established endothelial cell junctions. PMID: 24621569
- FOXF1 repressed cell growth and expression of collagen-1 and actin-related protein 2/3 complex, subunit 2. PMID: 25260753
- The Arp2/3 complex is recruited to invading Rickettsia parkeri and is required for efficient invasion. PMID: 22188208
- Filopodia initiation: focus on the Arp2/3 complex and formins PMID: 21975549
- WASH is a bimodular protein and a component of the BLOC-1 complex in which the C terminus is involved in Arp2/3-mediated actin nucleation, whereas the N-terminal portion is required for its regulation and localization in the cells PMID: 20308062
- The actin-related protein 2 accumulated in punctate structures that formed an extensivenetwork in a region corresponding to the postition of the Golgi complex. PMID: 15793564
- Compared with controls, actin-related protein 2/3 level was markedly increased in brains of intractable epilepsy patients. PMID: 18708039
- These findings strongly suggest that defective IL10 function is central to the pathogenesis of the ulcerative colitis subtype of inflammatory bowel disease. PMID: 18836448
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亞細胞定位：Cytoplasm, cytoskeleton. Cell projection. Cell junction, synapse, synaptosome. Nucleus.
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蛋白家族：ARPC2 family
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數據庫鏈接：
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