pagA Antibody

說明書

中文名稱：

pagA Antibody
貨號：

CSB-PA320223XA01BQE
規格：

￥880
圖片：
Western Blot
Positive WB detected in: recombinant protein
All lanes:pagA Antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/20000 dilution
Predicted band size: 89.6 kDa
Observed band size: 90 kDa
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品名稱：

Rabbit anti-Bacillus anthracis pagA Polyclonal antibody
Uniprot No.：

P13423
基因名：

pagA
別名：

Protective antigen (PA) (Anthrax toxins translocating protein) (PA-83) (PA83) [Cleaved into: Protective antigen PA-20 (PA20), Protective antigen PA-63 (PA63)], pagA, pag
宿主：

Rabbit
反應種屬：

Bacillus anthracis
免疫原：

Recombinant Bacillus anthracis pagA protein (30-764aa)
免疫原種屬：

Bacillus anthracis
標記方式：

Non-conjugated
克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

Antigen Affinity Purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, WB

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Protective antigen constitutes one of the three proteins composing the anthrax toxin; it mediates attachment to host cells and translocation of edema factor (EF) and lethal factor (LF) into the host cytoplasm. PA associated with LF forms the lethal toxin (LeTx) and causes death when injected; PA associated with EF forms the edema toxin (EdTx) and produces edema. PA induces immunity to infection with anthrax.; Mediates the attachment to host cells by binding host cell receptors ANTXR1 and ANTXR2. Following host cell surface attachment, PA is cleaved by FURIN to generate the PA-63 (Protective antigen PA-63) form, which constitutes the mature form of the protein that oligomerizes and forms a pore to translocate the enzymatic toxin components edema factor (EF) and lethal factor (LF) into the host cytosol.; Mature form that oligomerizes and forms a pore to translocate the enzymatic toxin components edema factor (EF) and lethal factor (LF) into the host cytosol. Following attachment to host cell receptors and cleavage by FURIN, homooligomerizes to form ring-shaped oligomers that are in a pre-pore conformation, and associates with EF and LF. Toxin-leaded complexes are then endocytosed in a clathrin-dependent process, followed by a conformational change of oligomerized PA-63 from the pre-pore to pore state, which is triggered by the low pH in the endosome. Once active, the pore mediates unfolding of EF and LF, which pass through the pore and translocate into the host cytosol.
基因功能參考文獻：
1. PA is a calcium-dependent serine protease and that the protein potentially uses this proteolytic activity for receptor binding. PMID: 30052476
2. Stability of domain 4 of the anthrax toxin protective antigen and the effect of the VWA domain of CMG2 on stability PMID: 27874231
3. Data show that both mutant and native protective antigen vaccine preparations protected rabbits from lethal, aerosolized, spore challenge. PMID: 26207820
4. Data suggest that anthrax protective antigen (PA) expressing Semliki Forest virus (SFV) replicon DNA or viral replicon particles (VRP) vaccines may be suitable as candidate vaccines against anthrax. PMID: 25102364
5. N435C mutantion of anthrax protective antigen conferred dominant-negative inhibitory activity. PMID: 20213183
6. analysis of linear epitopes in Bacillus anthracis protective antigen bound by neutralizing antibodies PMID: 19617628
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亞細胞定位：

[Protective antigen]: Secreted. Host cell membrane.; [Protective antigen PA-63]: Host cell membrane; Multi-pass membrane protein. Host endosome membrane; Multi-pass membrane protein.
蛋白家族：

Bacterial binary toxin family
數據庫鏈接：

KEGG: bar:GBAA_pXO1_0164

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