nusG Antibody

^{Rare Species}

說明書

中文名稱：

nusG兔多克隆抗體
貨號：

CSB-PA365293XA01ENV
規格：

￥880
圖片：
Western Blot
Positive WB detected in: recombinant protein
All lanes:nusGAntibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 23 kDa
Observed band size: 23 kDa
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品名稱：

Rabbit anti-Escherichia coli (strain K12) nusG Antibody Polyclonal antibody
Uniprot No.：

P0AFG0
基因名：

nusG Antibody
別名：

Transcription termination/antitermination protein NusG nusG b3982 JW3945
宿主：

Rabbit
反應種屬：

Escherichia coli (strain K12)
免疫原：

Recombinant Escherichia coli (strain K12) nusG protein (2-181aa)
免疫原種屬：

Escherichia coli (strain K12)
標記方式：

Non-conjugated
克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, WB

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Part of the processive rRNA transcription and antitermination complex (rrnTAC). The complex forms an RNA-chaperone ring around the RNA exit tunnel of RNA polymerase (RNAP). It supports rapid transcription and antitermination of rRNA operons, cotranscriptional rRNA folding, and annealing of distal rRNA regions to allow correct ribosome biogenesis. Participates in transcription elongation, termination and antitermination. In the absence of Rho, increases the rate of transcription elongation by the RNAP, probably by partially suppressing pausing. In the presence of Rho, modulates most Rho-dependent termination events by interacting with the RNAP to render the complex more susceptible to the termination activity of Rho. May be required to overcome a kinetic limitation of Rho to function at certain terminators. Also involved in ribosomal RNA and phage lambda N-mediated transcriptional antitermination.
基因功能參考文獻：
1. The comprehensive mapping of the upstream fork junction by base analogue fluorescence and nucleic acids crosslinking suggests that NusG inhibits backtracking by stabilizing the minimal transcription bubble. PMID: 27697152
2. NusG acts as both a positive and a negative regulator of Rho in the course of the bacterial transcription termination. (Review) PMID: 27023849
3. NusG is required for efficient termination at minority subsets (~20%) of both antisense and sense Rho-dependent terminators with lower C/G ratio sequences PMID: 23207917
4. E. coli NusG inhibits backtracking and accelerates pause-free transcription by promoting forward translocation of RNA polymerase. PMID: 20381500
5. findings suggest that the mini-domain serves as a structural element for an interaction rather than as a site for residue-specific contacts PMID: 15890417
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蛋白家族：

NusG family
數據庫鏈接：

KEGG: ecj:JW3945

STRING: 316385.ECDH10B_4170

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