lexA Antibody

^{Rare Species}

中文名稱：

lexA兔多克隆抗體
貨號：

CSB-PA363985HA01ENV
規格：

￥440
圖片：
Western Blot
Positive WB detected in Recombinant protein
All lanes: lexA antibody at 3.4μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
predicted band size: 39 kDa
observed band size: 39 kDa
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品描述：

The LexA antibody is prepared through the immunization of rabbits with the peptide corresponding to amino acids 1-202 of the recombinant E.coli LexA repressor protein and further purification from rabbit antiserum by protein G. It is a polyclonal antibody and exists as an unconjugated IgG. Its purity reaches up to 95%. It only reacts with the LexA protein of E.coli-origin. And this anti-LexA antibody has been validated for use in ELISA and WB analyses. In E.coli, the transcriptional repressor LexA controls the SOS response, which is a coordinated cellular response to DNA damage. The LexA protein inhibits gene expression by binding as a dimer to a 16-mer consensus sequence CTG-N10-CAG named SOS boxes.
產品名稱：

Rabbit anti-Escherichia coli lexA Polyclonal antibody
Uniprot No.：

P0A7C2
基因名：

lexA
別名：

lexA antibody; exrA antibody; spr antibody; tsl antibody; umuA antibody; b4043 antibody; JW4003LexA repressor antibody; EC 3.4.21.88 antibody
宿主：

Rabbit
反應種屬：

Escherichia coli
免疫原：

Recombinant Escherichia coli LexA repressor protein (1-202AA)
免疫原種屬：

Escherichia coli

標記方式：

Non-conjugated

本頁面中的產品，lexA Antibody (CSB-PA363985HA01ENV)，的標記方式是Non-conjugated。對于lexA Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產品名稱	應用
HRP	CSB-PA363985HB01ENV	lexA Antibody, HRP conjugated	ELISA
FITC	CSB-PA363985HC01ENV	lexA Antibody, FITC conjugated
Biotin	CSB-PA363985HD01ENV	lexA Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, WB

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. Binds to the 16 bp palindromic sequence 5'-CTGTATATATATACAG-3'. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair. Implicated in hydroxy radical-mediated cell death induced by hydroxyurea treatment.The SOS response controls an apoptotic-like death (ALD) induced (in the absence of the mazE-mazF toxin-antitoxin module) in response to DNA damaging agents that is mediated by RecA and LexA.
基因功能參考文獻：
1. The conformational flexibility of unbound LexA is the key element in establishing a co-ordinated SOS response. PMID: 21576225
2. LexA regulated genes exhibit phenotypic heterogeneity as high level expression is observed in only a small subpopulation. PMID: 21070632
3. NMR analysis of LexA catalytic domain in its active conformation PMID: 15929009
4. oligomerization of LexA is proposed to be a possible regulation mechanism of the Escherichia coli SOS regulon in response to environmental conditions resulting in acidic intracellular pH. Structural changes on LexA repressor promoted by acidic pH. PMID: 16701697
5. LexA binds to and represses the promoter of antC of coliphage N15. PMID: 17586637
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蛋白家族：

Peptidase S24 family
數據庫鏈接：

KEGG: ecj:JW4003

STRING: 316385.ECDH10B_4232

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