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中文名稱：lexA兔多克隆抗體
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貨號：CSB-PA363985HA01ENV
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規格：￥440
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圖片：
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Western Blot
Positive WB detected in Recombinant protein
All lanes: lexA antibody at 3.4μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
predicted band size: 39 kDa
observed band size: 39 kDa
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其他：
產品詳情
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產品描述：
The LexA antibody is prepared through the immunization of rabbits with the peptide corresponding to amino acids 1-202 of the recombinant E.coli LexA repressor protein and further purification from rabbit antiserum by protein G. It is a polyclonal antibody and exists as an unconjugated IgG. Its purity reaches up to 95%. It only reacts with the LexA protein of E.coli-origin. And this anti-LexA antibody has been validated for use in ELISA and WB analyses. In E.coli, the transcriptional repressor LexA controls the SOS response, which is a coordinated cellular response to DNA damage. The LexA protein inhibits gene expression by binding as a dimer to a 16-mer consensus sequence CTG-N10-CAG named SOS boxes.
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產品名稱：Rabbit anti-Escherichia coli lexA Polyclonal antibody
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Uniprot No.：
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基因名：lexA
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別名：lexA antibody; exrA antibody; spr antibody; tsl antibody; umuA antibody; b4043 antibody; JW4003LexA repressor antibody; EC 3.4.21.88 antibody
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宿主：Rabbit
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反應種屬：Escherichia coli
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免疫原：Recombinant Escherichia coli LexA repressor protein (1-202AA)
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免疫原種屬：Escherichia coli
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標記方式：Non-conjugated
本頁面中的產品，lexA Antibody (CSB-PA363985HA01ENV)，的標記方式是Non-conjugated。對于lexA Antibody，我們還提供其他標記。見下表：
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克隆類型：Polyclonal
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抗體亞型：IgG
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純化方式：>95%, Protein G purified
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 -
產品提供形式：Liquid
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應用范圍：ELISA, WB
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推薦稀釋比：
Application Recommended Dilution WB 1:500-1:5000 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產品評價
相關產品
靶點詳情
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功能：Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. Binds to the 16 bp palindromic sequence 5'-CTGTATATATATACAG-3'. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair. Implicated in hydroxy radical-mediated cell death induced by hydroxyurea treatment.The SOS response controls an apoptotic-like death (ALD) induced (in the absence of the mazE-mazF toxin-antitoxin module) in response to DNA damaging agents that is mediated by RecA and LexA.
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基因功能參考文獻：
- The conformational flexibility of unbound LexA is the key element in establishing a co-ordinated SOS response. PMID: 21576225
- LexA regulated genes exhibit phenotypic heterogeneity as high level expression is observed in only a small subpopulation. PMID: 21070632
- NMR analysis of LexA catalytic domain in its active conformation PMID: 15929009
- oligomerization of LexA is proposed to be a possible regulation mechanism of the Escherichia coli SOS regulon in response to environmental conditions resulting in acidic intracellular pH. Structural changes on LexA repressor promoted by acidic pH. PMID: 16701697
- LexA binds to and represses the promoter of antC of coliphage N15. PMID: 17586637
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蛋白家族：Peptidase S24 family
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數據庫鏈接：
KEGG: ecj:JW4003
STRING: 316385.ECDH10B_4232
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