VRK1 Antibody

說明書

中文名稱：

VRK1兔多克隆抗體
貨號：

CSB-PA857466DSR1HU
規格：

￥440
圖片：
Western blot
All lanes: DCXR antibody at 4.74μg/ml
Lane 1: MCF-7 whole cell lysate
Lane 2: Hela whole cell lysate
Lane 3: A549 whole cell lysate
Lane 4: 293T whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDa

Immunohistochemistry of paraffin-embedded human lung cancer using CSB-PA857466DSR1HU at dilution of 1:100

Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA857466DSR1HU at dilution of 1:100
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) VRK1 Polyclonal antibody
Uniprot No.：

Q99986
基因名：

VRK1
別名：

MGC117401 antibody; MGC138280 antibody; MGC142070 antibody; PCH1 antibody; PCH1A antibody; Serine/threonine protein kinase VRK1 antibody; Serine/threonine-protein kinase VRK1 antibody; Vaccinia related kinase 1 antibody; Vaccinia virus B1R related kinase 1 antibody; Vaccinia-related kinase 1 antibody; VRK1 antibody; VRK1_HUMAN antibody
宿主：

Rabbit
反應種屬：

Human
免疫原：

Recombinant Human Serine/threonine-protein kinase VRK1 protein (257-396AA)
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

Antigen Affinity Purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC

推薦稀釋比：

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:20-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Serine/threonine kinase involved in Golgi disassembly during the cell cycle: following phosphorylation by PLK3 during mitosis, required to induce Golgi fragmentation. Acts by mediating phosphorylation of downstream target protein. Phosphorylates 'Thr-18' of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2. Phosphorylates casein and histone H3. Phosphorylates BANF1: disrupts its ability to bind DNA, reduces its binding to LEM domain-containing proteins and causes its relocalization from the nucleus to the cytoplasm. Phosphorylates ATF2 which activates its transcriptional activity.
基因功能參考文獻：
1. that knockdown of VRK1 would inhibit the proliferation of glioma cells PMID: 29103766
2. this study not only provides a resource for investigating the unidentified functions of VRK1/VRK3, but also an insight into the regulatory roles of VRK1/VRK3 in biological processes. PMID: 28927264
3. Block the activation of the VRK1 promoter by Sox2. PMID: 27334688
4. VRK1 and BANF1 yielded AUCs of 0.790 and 0.735, respectively. PMID: 28298069
5. VRK1 AND VRK2 expression are predictive of tumor response to neoadjuvant chemoradiation therapy in rectal adenocarcinoma. PMID: 27456229
6. VRK1 was lowly expressed in adherent MM cells and highly expressed in suspended cells. In addition, VRK1 was positively correlated with the proliferation of MM cells by regulating the expression of cell cycle-related protein, such as cyclinD1, CDK2 and p27(kip1). PMID: 27319807
7. we conclude that VRK1 may be a candidate prognostic biomarker as well as a potential therapeutical target of hepatocellular carcinoma PMID: 26706601
8. VRK1 may act as a tumor promoter by controlling the level of cell cycle regulators associated with G1/S transition and could potentially serve as a therapeutic target and/or prognostic biomarker for HCC. PMID: 26375549
9. VRK1 is a novel regulator of CBs dynamics and stability in cell cycle by protecting coilin from ubiquitination and degradation in the proteasome, and propose a model of CB dynamics. PMID: 26068304
10. VRK1 regulation of NBS1 contributes to the stability of the repair complex and permits the sequential steps in DNA damage response. PMID: 26869104
11. VRK1 is a novel chromatin component that reacts to its alterations and participates very early in DNA damage response, functioning by itself or in cooperation with Ataxia Telangiectasia Mutated Proteins. PMID: 25923214
12. VRK1 deficiency in human and mouse leads to downregulation of amyloid-beta precursor protein (APP). APP overexpression rescues the phenotype caused by Vrk1 knockdown, suggesting that VRK1 affects neuronal migration through an APP-dependent mechanism. PMID: 25609612
13. VRK1 can contribute to make these tumors more resistant to DNA damage-based therapies, such as ionizing radiation or doxorubicin, which is consistent with its association to a poor prognosis in ER positive breast cancer. PMID: 24731990
14. VRK1 deficiency disrupts nuclear envelope morphology and leads to BAF retention on mitotic chromosomes. PMID: 24430874
15. The VRK1-p53 basal complex is an early-warning system for immediate cellular responses to DNA damage. PMID: 24492002
16. This study identification of disease-causing alleles in 3 children from 2 unrelated families with Mutations in VRK1 associated with complex motor and sensory axonal neuropathy plus microcephaly. PMID: 24126608
17. Data from molecular dynamic simulations suggest that bivalent cations play key structural roles in stabilization of VRK1/VRK2; bivalent cations have no obvious effects on VRK3. These kinases are targets in search for antineoplastic agents. [REVIEW] PMID: 23082977
18. MKP2 is a negative regulator of VRK1-mediated histone H3 phosphorylation. PMID: 23223570
19. Data show that vaccinia-related kinase 1 (VRK1) in resting cells plays an important role in the formation of ionizing radiation-induced foci that assemble on the 53BP1 scaffold protein during the DNA damage response. PMID: 22621922
20. direct interaction and binding features between VRK1 and macroH2A1.2 PMID: 22194607
21. VRK1 interacts and phosphorylates coilin, a protein regulating the assembly of Cajal bodies in the nucleus. PMID: 21920476
22. NMR solution structure of a catalytically active form of human VRK1 PMID: 21543316
23. VRK1 downregulation is necessary to modulate the block in cell cycle progression induced by p53 as part of its DNA damage response. PMID: 21386980
24. vrk1 gene mutation can be excluded in 2 Chinese pedigrees of the first and second branchial arch syndrome. PMID: 18257281
25. can perform the functions of B1 kinase required for vaccinia virus genome replication, most likely due to overlapping specificity for cellular and/or viral substrates PMID: 14747564
26. VRK1 phosphorylates ATF2 and is an element of a novel signaling pathway that regulates gene transcription. PMID: 15105425
27. c-Jun is phosphorylated by the human vaccinia-related kinase 1 (VRK1) and cooperates with the N-terminal kinase of c-Jun (JNK) PMID: 15378002
28. VRK1 is the first step in a new pathway regulating p53 activity during cell proliferation PMID: 15542844
29. VRK1 might play a role in cell cycle regulation and is likely to represent the beginning of a new control mechanism of cell cycle, particularly late in the G1-S phase. PMID: 16547155
30. As the amount of p53 protein increases, there is a downregulation of the VRK1 protein level independent of its promoter. PMID: 16782868
31. The different reactivity of the VRK1 protein indicates that this protein has a subcellular localization that can be regulated, thus adding an additional level of regulatory complexity to the VRK1 protein. PMID: 17617371
32. accumulation of VRK1 in tumours with mutant p53 could result in stimulation of other signalling pathways PMID: 17689819
33. These findings collectively support a role of VRK1 as a novel mitotic histone H3 kinase in mammals. PMID: 17938195
34. Small interference RNAs specific for Vrk1, was identified that reproducibly reduce viral RNA and viral protein levels in hepatitis C virus replicon-bearing cells. PMID: 17951261
35. VRK1 is an immediate early response gene required for entry in G1 PMID: 18286197
36. the downregulation of VRK1 protein levels, as a consequence of p53 accumulation, is thus dependent on the levels of the p300/CBP protein available for transcriptional complexes PMID: 18612383
37. Ran is a novel negative regulator of nuclear VRK1 and VRK2 kinase activity, which may vary in different subcellular localizations generating an asymmetric intracellular distribution of kinase activity depending on local protein interactions. PMID: 18617507
38. Results show that VRK1 activates CREB and regulates cell cycle progression in the DNA replication period by inducing cyclin D1 (CCND1) expression. PMID: 18713830
39. The Plk3-VRK1 kinase module might represent two consecutive steps of a signaling cascade that participates in the regulation of Golgi fragmentation. PMID: 19103756
40. Spinal muscular atrophy with pontocerebellar hypoplasia is caused by a mutation in the VRK1 gene. PMID: 19646678
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相關疾病：

Pontocerebellar hypoplasia 1A (PCH1A)
亞細胞定位：

Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, spindle. Note=Dispersed throughout the cell but not located on mitotic spindle or chromatids during mitosis.
蛋白家族：

Protein kinase superfamily, CK1 Ser/Thr protein kinase family, VRK subfamily
組織特異性：

Widely expressed. Highly expressed in fetal liver, testis and thymus.
數據庫鏈接：

HGNC: 12718

OMIM: 602168

KEGG: hsa:7443

STRING: 9606.ENSP00000216639

UniGene: Hs.422662

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