UNC13B Antibody

中文名稱：

UNC13B兔多克隆抗體
貨號：

CSB-PA025622LA01HU
規格：

￥440
圖片：
IHC image of CSB-PA025622LA01HU diluted at 1:300 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of Hela cells with CSB-PA025622LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Western Blot
Positive WB detected in: U251 whole cell lysate, MCF-7 whole cell lysate
All lanes: UNC13B antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 181, 183 kDa
Observed band size: 181 kDa
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) UNC13B Polyclonal antibody
Uniprot No.：

O14795
基因名：

UNC13B
別名：

hmunc13 antibody; MGC133279 antibody; MGC133280 antibody; munc13 antibody; Munc13-2 antibody; Protein unc-13 homolog B antibody; UN13B_HUMAN antibody; unc-13 homolog B (C. elegans) antibody; UNC13 antibody; Unc13b antibody; Unc13h2 antibody
宿主：

Rabbit
反應種屬：

Human
免疫原：

Recombinant Human Protein unc-13 homolog B protein (12-215AA)
免疫原種屬：

Homo sapiens (Human)

標記方式：

Non-conjugated

本頁面中的產品，UNC13B Antibody (CSB-PA025622LA01HU)，的標記方式是Non-conjugated。對于UNC13B Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產品名稱	應用
HRP	CSB-PA025622LB01HU	UNC13B Antibody, HRP conjugated	ELISA
FITC	CSB-PA025622LC01HU	UNC13B Antibody, FITC conjugated
Biotin	CSB-PA025622LD01HU	UNC13B Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC, IF

推薦稀釋比：

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:200-1:500
IF	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Plays a role in vesicle maturation during exocytosis as a target of the diacylglycerol second messenger pathway. Is involved in neurotransmitter release by acting in synaptic vesicle priming prior to vesicle fusion and participates in the activity-depending refilling of readily releasable vesicle pool (RRP). Essential for synaptic vesicle maturation in a subset of excitatory/glutamatergic but not inhibitory/GABA-mediated synapses. In collaboration with UNC13A, facilitates neuronal dense core vesicles fusion as well as controls the location and efficiency of their synaptic release.
基因功能參考文獻：
1. A rare missense variation (V1525M) in UNC13B was identified by WES in the multiplex family; this variation was present in five of six affected individuals, but not in eight unaffected individuals or one individual of unknown disease status. Resequencing UNC13B coding regions identified five rare missense variations (T103M, M813T, P1349T, I1362T, and V1525M). PMID: 26990377
2. The 1-5-8-26 CaM binding motif discovered in Munc13-1 cannot be induced in the classical CaM target skMLCK, indicating unique features of the Munc13 CaM binding motif. PMID: 24854537
3. Munc13-1 regulates insulin exocytosis PMID: 12871971
4. MUNC13-4 mutations play a role in the development of familial haemophagocytic lymphohistiocytosis subtype 3 through a defective cytotoxic pathway PMID: 15466010
5. Data suggest that diacylglycerol-activated hmunc13 serves as an effector of Rab34, mediating lysosome-Golgi trafficking. PMID: 16138900
6. Data identified a polymorphism in the UNC13B gene associated with nephropathy. UNC13B mediates apopotosis in glomerular cells in the presence of hyperglycemia, an event occurring early in the development of nephropathy. PMID: 18633107
7. In the bound state, the hydrophobic anchor residue of the calmodulin (CaM)-binding motif in Munc13 contacts two distinct methionine residues in the carboxyl-terminal domain of CaM. PMID: 19492809
8. mechanistic basis for high glucose-induced protein secretion is through interaction of munc13 and rab34, indicating a potentially critical role for this newly described pathway in the pathogenesis of DN. PMID: 19641095
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亞細胞定位：

Cytoplasm. Membrane; Peripheral membrane protein. Cell membrane. Cell junction, synapse.
蛋白家族：

Unc-13 family
組織特異性：

Expressed in kidney cortical epithelial cells and brain.
數據庫鏈接：

HGNC: 12566

OMIM: 605836

KEGG: hsa:10497

STRING: 9606.ENSP00000367756

UniGene: Hs.493791

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