UBE2K Antibody

中文名稱：

UBE2K兔多克隆抗體
貨號：

CSB-PA09667A0Rb
規格：

￥440
圖片：
Immunohistochemistry of paraffin-embedded human placenta tissue using CSB-PA09667A0Rb at dilution of 1:20

Immunofluorescence staining of Hela cells with CSB-PA09667A0Rb at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
其他：

產品詳情
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靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) UBE2K Polyclonal antibody
Uniprot No.：

P61086
基因名：

UBE2K
別名：

E2 25K antibody; E2(25K) antibody; HIP-2 antibody; Huntingtin interacting protein 2 antibody; Huntingtin-interacting protein 2 antibody; HYPG antibody; LIG antibody; UBC1 antibody; UBE2K antibody; UBE2K_HUMAN antibody; Ubiquitin carrier protein antibody; Ubiquitin conjugating enzyme E2 25 kDa antibody; ubiquitin conjugating enzyme E2K antibody; ubiquitin conjugating enzyme E2K (UBC1 homolog, yeast) antibody; Ubiquitin protein ligase antibody; Ubiquitin-conjugating enzyme E2 K antibody; Ubiquitin-conjugating enzyme E2(25K) antibody; Ubiquitin-conjugating enzyme E2-25 kDa antibody; Ubiquitin-conjugating enzyme E2-25K antibody; Ubiquitin-protein ligase antibody
宿主：

Rabbit
反應種屬：

Human
免疫原：

Recombinant Human Ubiquitin-conjugating enzyme E2 K protein (2-200AA)
免疫原種屬：

Homo sapiens (Human)

標記方式：

Non-conjugated

本頁面中的產品，UBE2K Antibody (CSB-PA09667A0Rb)，的標記方式是Non-conjugated。對于UBE2K Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產品名稱	應用
HRP	CSB-PA09667B0Rb	UBE2K Antibody, HRP conjugated	ELISA
FITC	CSB-PA09667C0Rb	UBE2K Antibody, FITC conjugated
Biotin	CSB-PA09667D0Rb	UBE2K Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, IHC, IF

推薦稀釋比：

Application	Recommended Dilution
IHC	1:10-1:100
IF	1:50-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro, in the presence or in the absence of BRCA1-BARD1 E3 ubiquitin-protein ligase complex, catalyzes the synthesis of 'Lys-48'-linked polyubiquitin chains. Does not transfer ubiquitin directly to but elongates monoubiquitinated substrate protein. Mediates the selective degradation of short-lived and abnormal proteins, such as the endoplasmic reticulum-associated degradation (ERAD) of misfolded lumenal proteins. Ubiquitinates huntingtin. May mediate foam cell formation by the suppression of apoptosis of lipid-bearing macrophages through ubiquitination and subsequence degradation of p53/TP53. Proposed to be involved in ubiquitination and proteolytic processing of NF-kappa-B; in vitro supports ubiquitination of NFKB1. In case of infection by cytomegaloviruses may be involved in the US11-dependent degradation of MHC class I heavy chains following their export from the ER to the cytosol. In case of viral infections may be involved in the HPV E7 protein-dependent degradation of RB1.
基因功能參考文獻：
1. The crystal structure of a Ube2K~ubiquitin conjugate has been described, and it was shown that even though it is monomeric, Ube2K can synthesize Lys48-linked ubiquitin chains. PMID: 26592444
2. Hip2, a ubiquitin-conjugating enzyme, can overcome radiation-induced G2/M cell cycle arrest and trigger the entry into mitosis. PMID: 23933584
3. An extract of bark from the tropical rainforest plant Byrsonima crassifolia was screened for inhibition of diubiquitin formation by the human ubiquitin-conjugating enzyme E2-25K. PMID: 22164771
4. one of the roles of the C-terminal UBA domain, in the context of E2-25K, is to increase processivity in Lys48-linked polyubiquitin chain synthesis, possibly through increased binding to the ubiquitinated substrate. PMID: 21281599
5. Results suggest that the interaction between E2-25K and UBB(+1) is critical for the synthesis and accumulation of UBB(+1)-anchored polyubiquitin, which results in proteasomal inhibition and neuronal cell death. PMID: 20826778
6. This study suggests that Hip2 might be involved in the regulation of Smac-mediated apoptosis. PMID: 20537984
7. Results suggest that in permeabilized, US11-expressing cells polyubiquitination of the MHC class I heavy chain substrate can be catalyzed by E2-25K. PMID: 16868077
8. E2-25K is involved in aggregate formation of expanded polyglutamine proteins and polyglutamine-induced cell death. PMID: 17092742
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亞細胞定位：

Cytoplasm.
蛋白家族：

Ubiquitin-conjugating enzyme family
組織特異性：

Expressed in all tissues tested, including spleen, thymus, prostate, testis, ovary, small intestine, colon, peripheral blood leukocytes, T-lymphocytes, monocytes, granulocytes and bone marrow mononuclear cells. Highly expressed in brain, with highest leve
數據庫鏈接：

HGNC: 4914

OMIM: 602846

KEGG: hsa:3093

STRING: 9606.ENSP00000261427

UniGene: Hs.50308

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