TPST1 Antibody

說明書

中文名稱：

TPST1兔多克隆抗體
貨號：

CSB-PA024132LA01HU
規格：

￥3,900
圖片：
IHC image of CSB-PA024132LA01HU diluted at 1:50 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody

IHC image of CSB-PA024132LA01HU diluted at 1:50 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody

Western Blot
Positive WB detected in: Mouse Skeletal Muscle tissue lysate(20μg),Rat Skeletal Muscle tissue lysate(20μg)
All lanes: TPST1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 43 kDa
Observed band size: 43 kDa
Exposure time: 120s

Western Blot
Positive WB detected in: A549 whole cell lysate(20μg), U251 whole cell lysate(20μg), HepG2 whole cell lysate(20μg), Hela whole cell lysate(20μg), PC-3 whole cell lysate(20μg), 293 whole cell lysate(20μg)
All lanes: TPST1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/20000 dilution
Predicted band size: 43 kDa
Observed band size: 43 kDa
Exposure time: 60s
其他：

產品詳情
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相關產品
靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) TPST1 Polyclonal antibody
Uniprot No.：

O60507
基因名：

TPST1
別名：

TPST1; Protein-tyrosine sulfotransferase 1; Tyrosylprotein sulfotransferase 1; TPST-1
宿主：

Rabbit
反應種屬：

Human, Mouse, Rat
免疫原：

Recombinant Human Protein-tyrosine sulfotransferase 1 protein (283-370AA)
免疫原種屬：

Homo sapiens (Human)
克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

Antigen?affinity?purification
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.02% sodium azide
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC

推薦稀釋比：

Application	Recommended Dilution
WB	1:1000-1:3000
IHC	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Catalyzes the O-sulfation of tyrosine residues within acidic motifs of polypeptides, using 3'-phosphoadenylyl sulfate (PAPS) as cosubstrate.
基因功能參考文獻：
1. TPST1 was significantly negatively correlated with the expression of cMet in lung cancer and may be a negative prognostic biomarker of lung cancer. PMID: 26238632
2. TPST1 rs3757417T>G polymorphisms are associated with colorectal cancer. PMID: 25079514
3. Affinity purified salivary TPST showed a single band of 50-54 kDa and is the first report characterizing a tyrosylprotein sulfotransferase in secretory fluid from the parotid gland PMID: 16244708
4. results exclude TPST1 as the causative gene for Shwachman-Diamond syndrome PMID: 12032733
5. Shear stress-dependent downregulation of TPST1 in human endothelium involves protein kinase C PMID: 12056800
6. Human tyrosylprotein sulfotransferase may be functional as homodimer/oligomer in the trans-Golgi compartment. PMID: 16859706
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亞細胞定位：

Golgi apparatus membrane; Single-pass type II membrane protein.
蛋白家族：

Protein sulfotransferase family
組織特異性：

Ubiquitous. Detected in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
數據庫鏈接：

HGNC: 12020

OMIM: 603125

KEGG: hsa:8460

STRING: 9606.ENSP00000302413

UniGene: Hs.421194

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