TPK1 Antibody

說明書

中文名稱：

TPK1兔多克隆抗體
貨號：

CSB-PA024103ESR2HU
規格：

￥440
圖片：
Western Blot
Positive WB detected in: U251 whole cell lysate(30μg), THP-1 whole cell lysate(30μg), MCF-7 whole cell lysate(30μg), K562 whole cell lysate(30μg), CT-26 whole cell lysate(30μg), PC-3 whole cell lysate(30μg),JK whole cell lysate(30μg),Mouse Liver tissue lysate(30μg),Mouse Kidneyr tissue lysate(30μg)
All lanes: TPK1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 27,14kDa
Observed band size: 27 kDa
Exposure time: 180s

IHC image of CSB-PA024103ESR2HU diluted at 1:100 and staining in paraffin-embedded human Kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody

IHC image of CSB-PA024103ESR2HU diluted at 1:100 and staining in paraffin-embedded human Heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody

IHC image of CSB-PA024103ESR2HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) TPK1 Polyclonal antibody
Uniprot No.：

Q9H3S4
基因名：

TPK1
別名：

hTPK1 antibody; Placental protein 20 antibody; PP20 antibody; Thiamin pyrophosphokinase 1 antibody; Thiamine diphosphokinase antibody; Thiamine kinase antibody; Thiamine pyrophosphokinase 1 antibody; THMD5 antibody; TPK1 antibody; TPK1_HUMAN antibody
宿主：

Rabbit
反應種屬：

Human, Mouse
免疫原：

Synthesized peptide derived from human TPK1:170-220aa
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

Antigen Affinity Purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:20-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Catalyzes the phosphorylation of thiamine to thiamine pyrophosphate. Can also catalyze the phosphorylation of pyrithiamine to pyrithiamine pyrophosphate.
基因功能參考文獻：
1. Mutation analysis of TPK1 uncovered three missense, one splice-site, and one frameshift mutation resulting in decreased TPK protein levels PMID: 22152682
2. findings indicate the importance of the Sp1 transcription factor cis-element in regulating the human thiamin pyrophosphokinase gene (hTPK1) gene expression PMID: 16262001
3. Genomic variations in either the fetal or maternal TPK1 gene could contribute to variability of birth weight in normal humans. PMID: 17295612
4. This is the first report on the primary sequence for mammalian thiamin pyrophosphokinase, a protein that catalyzes the pyrophosphorylation of thiamin with adenosine 5'-triphosphate to form thiamin pyrophosphate. PMID: 10567383
相關疾病：

Thiamine metabolism dysfunction syndrome 5, episodic encephalopathy type (THMD5)
蛋白家族：

Thiamine pyrophosphokinase family
組織特異性：

Detected in heart, kidney, testis, small intestine and peripheral blood leukocytes, and at very low levels in a variety of tissues.
數據庫鏈接：

HGNC: 17358

OMIM: 606370

KEGG: hsa:27010

STRING: 9606.ENSP00000353165

UniGene: Hs.660232

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