TOP3B Antibody
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中文名稱：TOP3B兔多克隆抗體
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貨號：CSB-PA004316
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規格：￥1090
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圖片：
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Western Blot analysis of HuvEc cells using Topo IIIβ-1 Polyclonal Antibody
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其他：
產品詳情
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Uniprot No.：
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基因名：TOP3B
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別名：DNA topoisomerase 3 beta 1 antibody; DNA topoisomerase 3-beta-1 antibody; DNA topoisomerase III beta 1 antibody; DNA topoisomerase III beta-1 antibody; FLJ39376 antibody; TOP 3B antibody; TOP3B antibody; TOP3B_HUMAN antibody; TOP3B1 antibody; Topo III beta antibody; Topoisomerase (DNA) III beta antibody; Topoisomerase DNA III beta antibody; Topoisomerase III beta antibody
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宿主：Rabbit
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反應種屬：Human,Mouse
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免疫原：Synthesized peptide derived from the N-terminal region of Human Topo IIIβ-1.
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免疫原種屬：Homo sapiens (Human)
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標記方式：Non-conjugated
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抗體亞型：IgG
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純化方式：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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產品提供形式：Liquid
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應用范圍：WB, IHC, ELISA
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推薦稀釋比：
Application Recommended Dilution WB 1:500-1:2000 IHC 1:100-1:300 ELISA 1:20000 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產品評價
相關產品
靶點詳情
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功能：Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. Possesses negatively supercoiled DNA relaxing activity.
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基因功能參考文獻：
- Structural basis of the interaction between TOP3B and the TDRD3 auxiliary factor has been reported. PMID: 28176834
- Results identified TOP3B gene deletion as candidate gene in the etiology of neurodevelopmental disorders including juvenile myoclonic epilepsy. PMID: 29490292
- Top3beta mutants lacking either its RNA-binding domain or catalytic residue fail to promote synapse formation, suggesting that Top3beta requires both its mRNA-binding and catalytic activity to facilitate neurodevelopment. PMID: 28039324
- Top3b proteins from several animals associate with polyribosomes, which are units of mRNA translation, whereas the Top3 homologs from E. coli and yeast lack the association. The Top3b-polyribosome association requires TDRD3, which directly interacts with Top3beta and is present in animals but not bacteria or yeast. PMID: 27257063
- TOP3B promotes transcription, protects against DNA damage, and reduces the frequency of chromosomal translocations. PMID: 24507716
- Topoisomerase IIIbeta expression can be a useful marker in assessing the prognosis of patients with breast cancer and is an independent predictor of survival. PMID: 20950730
- the positive regulatory region including the 5'GGAACC3' core element is essential for efficient expression of the DNA topoisomerase III beta gene PMID: 15358519
- Two isoforms of human DNA topoisomerase IIIbeta were expressed in HeLa cells. Isoform 1 was localized to the nucleus and associated with chromosomes during metaphase and anaphase. Isoform 2 was found in the cytoplasm. PMID: 11549288
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蛋白家族：Type IA topoisomerase family
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組織特異性：Isoform 1 is found in testis, heart and skeletal muscle. A 4 kb transcript which probably represents isoform 2 is found in thymus, kidney and pancreas.
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數據庫鏈接：
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