TMEM192 Antibody

說(shuō)明書(shū)

中文名稱(chēng)：

TMEM192兔多克隆抗體
貨號(hào)：

CSB-PA023784LA01HU
規(guī)格：

￥440
圖片：
Western Blot
Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate
All lanes: TMEM192 antibody at 3.2µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 31 kDa
Observed band size: 31 kDa

IHC image of CSB-PA023784LA01HU diluted at 1:400 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-PA023784LA01HU diluted at 1:400 and staining in paraffin-embedded human glioma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of A549 cells with CSB-PA023784LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunoprecipitating TMEM192 in Jurkat whole cell lysate
Lane 1: Rabbit control IgG (1µg) instead of CSB-PA023784LA01HU in Jurkat whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA023784LA01HU (6µg) + Jurkat whole cell lysate (500µg)
Lane 3: Jurkat whole cell lysate (10µg)
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

產(chǎn)品名稱(chēng)：

Rabbit anti-Homo sapiens (Human) TMEM192 Polyclonal antibody
Uniprot No.：

Q8IY95
基因名：

TMEM192
別名：

TMEM192; Transmembrane protein 192
宿主：

Rabbit
反應(yīng)種屬：

Human
免疫原：

Recombinant Human Transmembrane protein 192 protein (193-271AA)
免疫原種屬：

Homo sapiens (Human)

標(biāo)記方式：

Non-conjugated

本頁(yè)面中的產(chǎn)品，TMEM192 Antibody (CSB-PA023784LA01HU)，的標(biāo)記方式是Non-conjugated。對(duì)于TMEM192 Antibody，我們還提供其他標(biāo)記。見(jiàn)下表：

標(biāo)記方式	貨號(hào)	產(chǎn)品名稱(chēng)	應(yīng)用
HRP	CSB-PA023784LB01HU	TMEM192 Antibody, HRP conjugated	ELISA
FITC	CSB-PA023784LC01HU	TMEM192 Antibody, FITC conjugated
Biotin	CSB-PA023784LD01HU	TMEM192 Antibody, Biotin conjugated	ELISA

克隆類(lèi)型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, WB, IHC, IF, IP

推薦稀釋比：

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:200-1:500
IF	1:50-1:200
IP	1:200-1:2000

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Immunoprecipitation (IP) Protocol
儲(chǔ)存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

基因功能參考文獻(xiàn)：
1. Authors found that transmembrane protein 192 (TMEM192) interacted with TIG1. Authors also found that both TIG1A and TIG1B isoforms interacted and co-localized with TMEM192 in HtTA cervical cancer cells. The expression of TIG1 induced the expression of autophagy-related proteins. PMID: 27989102
2. TMEM192 deficiency can induce autophagy in tumor cells, and can further activate apoptosis by the mitochondrial pathway through autophagy. PMID: 22736246
3. TMEM192 was found to be strongly expressed in human kidney, liver, lung and pancreas tissue. The widespread tissue distribution could suggest an important role of TMEM192 for lysosomal function. PMID: 20370317
亞細(xì)胞定位：

Lysosome membrane; Multi-pass membrane protein. Late endosome.
蛋白家族：

TMEM192 family
組織特異性：

Strongly expressed in kidney, liver, lung and pancreas.
數(shù)據(jù)庫(kù)鏈接：

HGNC: 26775

KEGG: hsa:201931

STRING: 9606.ENSP00000305069

UniGene: Hs.704511

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