THEMIS2 Antibody

中文名稱：

THEMIS2兔多克隆抗體
貨號：

CSB-PA735984LA01HU
規(guī)格：

￥440
圖片：
Western Blot
Positive WB detected in: A549 whole cell lysate
All lanes: THEMIS2 antibody at 4.2µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 73, 30, 57, 14, 58, 51 kDa
Observed band size: 57 kDa

IHC image of CSB-PA735984LA01HU diluted at 1:400 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of NIH/3T3 cells with CSB-PA735984LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
其他：

產(chǎn)品詳情
產(chǎn)品評價
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靶點(diǎn)詳情

產(chǎn)品詳情

產(chǎn)品名稱：

Rabbit anti-Homo sapiens (Human) THEMIS2 Polyclonal antibody
Uniprot No.：

Q5TEJ8
基因名：

THEMIS2
別名：

Basement membrane induced antibody; Chromosome 1 open reading frame 38 antibody; ICB 1 antibody; ICB1 antibody; Induced by contact to basement membrane 1 protein antibody; Protein ICB 1 antibody; Protein ICB-1 antibody; Protein THEMIS2 antibody; THEMIS2 antibody; THMS2_HUMAN antibody; Thymocyte expressed molecule involved in selection protein 2 antibody; Thymocyte-expressed molecule involved in selection protein 2 antibody
宿主：

Rabbit
反應(yīng)種屬：

Human
免疫原：

Recombinant Human Protein THEMIS2 protein (62-177AA)
免疫原種屬：

Homo sapiens (Human)

標(biāo)記方式：

Non-conjugated

本頁面中的產(chǎn)品，THEMIS2 Antibody (CSB-PA735984LA01HU)，的標(biāo)記方式是Non-conjugated。對于THEMIS2 Antibody，我們還提供其他標(biāo)記。見下表：

標(biāo)記方式	貨號	產(chǎn)品名稱	應(yīng)用
HRP	CSB-PA735984LB01HU	THEMIS2 Antibody, HRP conjugated	ELISA
FITC	CSB-PA735984LC01HU	THEMIS2 Antibody, FITC conjugated
Biotin	CSB-PA735984LD01HU	THEMIS2 Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, WB, IHC, IF

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:200-1:500
IF	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點(diǎn)詳情

功能：

May constitute a control point in macrophage inflammatory response, promoting LPS-induced TLR4-mediated TNF production. Determines the threshold for activation of B cells by low-affinity and low-avidity ligands via PLCG2 activation and its downstream pathways.
基因功能參考文獻(xiàn)：
1. SNP rs1467465 of the human gene icb-1 might affect susceptibility to ovarian cancer. PMID: 24826199
2. Loss of icb-1 expression accelerated proliferation of SK-OV-3, OVCAR-3 and OAW-42 cells and led to upregulation of ovarian cancer biomarkers like KLK10 and CLDN16. PMID: 23474491
3. Data of this study suggest that icb-1 might exert a tumor-suppressor function in breast cancer and that its loss might confer relative resistance of breast cancer cells to apoptotic drugs. PMID: 22565810
4. Themis2 and Themis1 exhibit the same potential to restore T cell development and T cell receptor-mediated signaling in Themis1-deficient mice. PMID: 22732588
5. These findings together with the observed co-expression of icb-1 with E-cadherin in breast cancer samples support an important role of the icb-1 gene in cancer cell differentiation. PMID: 21455565
6. Data show that a highly significant positive correlation of icb-1 transcript levels with c-erbB2 (HER2) expression (p <.0001). PMID: 20569072
7. Identification of ICB-1 as a new player in endocrine-related cancer. PMID: 19942713
8. Increased icb-1 mRNA levels could be observed both in monocytic and granulocytic differentiation in maturing HL-60 cells. PMID: 12191572
9. The novel interferon-gamma inducible gene icb-1 is identified and its effects on the response of ovarian cancer cells to interferon-gamma are described PMID: 16219472
10. Data suggest that SNP rs1467465 of human gene icb-1 might affect breast cancer susceptibility. PMID: 19308812
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亞細(xì)胞定位：

Nucleus. Cytoplasm.
蛋白家族：

Themis family
組織特異性：

Expressed in different endometrial adenocarcinoma cell lines and various other cell lines apart from the prostate cell line LNCaP and the ovarian cancer cell line BG1.
數(shù)據(jù)庫鏈接：

HGNC: 16839

OMIM: 617856

KEGG: hsa:9473

STRING: 9606.ENSP00000363031

UniGene: Hs.10649

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