SSX2IP Antibody

中文名稱：

SSX2IP兔多克隆抗體
貨號(hào)：

CSB-PA897526LA01HU
規(guī)格：

￥440
圖片：
Western Blot
Positive WB detected in: Hela whole cell lysate, Rat liver tissue
All lanes: SSX2IP antibody at 3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 72, 66, 69 kDa
Observed band size: 72 kDa

IHC image of CSB-PA897526LA01HU diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-PA897526LA01HU diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of Hela cells with CSB-PA897526LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
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靶點(diǎn)詳情

產(chǎn)品詳情

產(chǎn)品名稱：

Rabbit anti-Homo sapiens (Human) SSX2IP Polyclonal antibody
Uniprot No.：

Q9Y2D8
基因名：

SSX2IP
別名：

ADIP antibody; ADIP_HUMAN antibody; Afadin and alpha actinin binding protein antibody; Afadin DIL domain interacting protein antibody; Afadin DIL domain-interacting protein antibody; Afadin- and alpha-actinin-binding protein antibody; FLJ10848 antibody; KIAA0923 antibody; MGC75026 antibody; OTTHUMP00000011574 antibody; SSX 2 interacting protein antibody; SSX2 interacting protein antibody; SSX2-interacting protein antibody; SSX2IP antibody; Synovial sarcoma X breakpoint 2 interacting protein antibody
宿主：

Rabbit
反應(yīng)種屬：

Human, Rat
免疫原：

Recombinant Human Afadin- and alpha-actinin-binding protein (485-614AA)
免疫原種屬：

Homo sapiens (Human)

標(biāo)記方式：

Non-conjugated

本頁(yè)面中的產(chǎn)品，SSX2IP Antibody (CSB-PA897526LA01HU)，的標(biāo)記方式是Non-conjugated。對(duì)于SSX2IP Antibody，我們還提供其他標(biāo)記。見下表：

標(biāo)記方式	貨號(hào)	產(chǎn)品名稱	應(yīng)用
HRP	CSB-PA897526LB01HU	SSX2IP Antibody, HRP conjugated	ELISA
FITC	CSB-PA897526LC01HU	SSX2IP Antibody, FITC conjugated
Biotin	CSB-PA897526LD01HU	SSX2IP Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, WB, IHC, IF

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:20-1:200
IF	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲(chǔ)存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Belongs to an adhesion system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs). May connect the nectin-afadin and E-cadherin-catenin system through alpha-actinin and may be involved in organization of the actin cytoskeleton at AJs through afadin and alpha-actinin. Involved in cell movement: localizes at the leading edge of moving cells in response to PDGF and is required for the formation of the leading edge and the promotion of cell movement, possibly via activation of Rac signaling. Acts as a centrosome maturation factor, probably by maintaining the integrity of the pericentriolar material and proper microtubule nucleation at mitotic spindle poles. The function seems to implicate at least in part WRAP73; the SSX2IP:WRAP73 complex is proposed to act as regulator of spindle anchoring at the mitotic centrosome. Involved in ciliogenesis. It is required for targeted recruitment of the BBSome, CEP290, RAB8, and SSTR3 to the cilia.
基因功能參考文獻(xiàn)：
1. This study determined the crystallographic structure of human Afadin in complex with LGN. PMID: 26751642
2. Studied afadin in complex with beta-catenin in glomeruli and in cultured mesangial cells. Found expression of afadin at mesangial intercellular junctions was greatly decreased in mesangial proliferative nephritis in rats & patients w/glomerulonephritis. PMID: 26568295
3. Knockdown of Wdr8 or hMsd1/SSX2IP displayed very similar mitotic defects, in which spindle microtubules became shortened and misoriented. PMID: 26545777
4. SSX2IP-mediated microtubule anchoring is essential for proper centriole assembly and duplication. On SSX2IP knockdown, the centriolar satellites become stuck at the microtubule minus end near the centrosome. PMID: 25833712
5. results showed that SSX2IP promotes the development and metastasis of hepatocellular carcinoma and contributes to the drug resistance of hepatoma cells PMID: 23452395
6. Nuclear protein SS2IP interacts with cancer-related protein SSX2 and the human homologue of a Ras-like GTPase interactor, RABIN3. PMID: 12007189
7. data shows that SSX2IP is affected in two ways that relate to the cell cycling of acute myeloid leukaemia cells PMID: 18028484
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亞細(xì)胞定位：

Cell junction, adherens junction. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriolar satellite. Cytoplasm, cytoskeleton, cilium basal body.
蛋白家族：

ADIP family
組織特異性：

Widely expressed, with the highest expression in brain, intermediate expression in kidney, testis, spinal cord, liver, heart, lung, skeletal muscle, ovary, fetal liver and fetal brain, and little to no expression in pancreas and spleen. All specific brain
數(shù)據(jù)庫(kù)鏈接：

HGNC: 16509

OMIM: 608690

KEGG: hsa:117178

STRING: 9606.ENSP00000340279

UniGene: Hs.22587

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