SPTLC2 Antibody

文獻引用 (1) 說明書

中文名稱：

SPTLC2兔多克隆抗體
貨號：

CSB-PA022640LA01HU
規格：

￥440
圖片：
Western blot
All lanes: SPTLC2 antibody at 12µg/ml
Lane 1: HepG2 whole cell lysate
Lane 2: A549 whole cell lysate
Lane 3: HT29 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 63 kDa
Observed band size: 63 kDa

Immunohistochemistry of paraffin-embedded human adrenal gland tissue using CSB-PA022640LA01HU at dilution of 1:100

Immunofluorescent analysis of HepG2 cells using CSB-PA022640LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
其他：

產品詳情
產品評價
引用文獻
相關產品
靶點詳情

產品詳情

產品描述：

The production of the SPTLC2 antibody starts by immunizing rabbits with the recombinant human SPTLC2 (88-562aa), follows by collecting the rabbit serum, and finishes by isolating and purifying the antibody from the rabbit serum through protein G affinity chromatography.

The resulting antibody got a high purity of up to 95%. It is only reactive with human SPTLC2 protein. This rabbit anti-human SPTLC2 polyclonal antibody has passed through quality verification in multiple applications, including ELISA, WB, IHC, and IF.
產品名稱：

Rabbit anti-Homo sapiens (Human) SPTLC2 Polyclonal antibody
Uniprot No.：

O15270
基因名：

SPTLC2
別名：

KIAA0526 antibody; LCB 2 antibody; LCB2 antibody; LCB2a antibody; Long chain base biosynthesis protein 2 antibody; Long chain base biosynthesis protein 2a antibody; Serine palmitoyl CoA transferase 2 antibody; Serine palmitoyltransferase 2 antibody; Serine palmitoyltransferase long chain base subunit 2 antibody; Serine palmitoyltransferase subunit II antibody; Serine-palmitoyl-CoA transferase 2 antibody; SPT 2 antibody; SPT2 antibody; SPTC2_HUMAN antibody; SPTLC 2 antibody; Sptlc2 antibody
宿主：

Rabbit
反應種屬：

Human
免疫原：

Recombinant Human Serine palmitoyltransferase 2 protein (88-562AA)
免疫原種屬：

Homo sapiens (Human)

標記方式：

Non-conjugated

本頁面中的產品，SPTLC2 Antibody (CSB-PA022640LA01HU)，的標記方式是Non-conjugated。對于SPTLC2 Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產品名稱	應用
HRP	CSB-PA022640LB01HU	SPTLC2 Antibody, HRP conjugated	ELISA
FITC	CSB-PA022640LC01HU	SPTLC2 Antibody, FITC conjugated
Biotin	CSB-PA022640LD01HU	SPTLC2 Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC, IF

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:20-1:200
IF	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

引用文獻

Expression of Ceramide-Metabolizing Enzymes in the Heart Adipose Tissue of Cardiovascular Disease Patients O Gruzdeva,International journal of molecular sciences,2023

產品評價

靶點詳情

功能：

Serine palmitoyltransferase (SPT). The heterodimer formed with LCB1/SPTLC1 constitutes the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC2-SPTSSA complex shows a strong preference for C16-CoA substrate, while the SPTLC1-SPTLC2-SPTSSB complex displays a preference for C18-CoA substrate. Plays an important role in de novo sphyngolipid biosynthesis which is crucial for adipogenesis.
基因功能參考文獻：
1. 2 families had late-onset autosomal dominant HSAN1C caused by a new variant in SPTLC2, c.547C>T, p.(Arg183Trp). The variant changed a conserved amino acid. PMID: 26573920
2. The activities of the hLCB2a mutants were measured in the presence of ssSPTa and ssSPTb and was found that all decrease enzyme activity. PMID: 24175284
3. Mutations in SPTLC2 are associated with increased deoxySL formation causing hereditary sensory and autonomic neuropathy type 1 (HSANI) in a familial study. PMID: 23658386
4. Mutations in the SPTLC2 subunit of serine palmitoyltransferase cause hereditary sensory and autonomic neuropathy type I. PMID: 20920666
5. results suggest that SPTLC2 mutations are not a common cause for genetic sensory neuropathies. PMID: 12207934
6. an increase in transepidermal water loss is an obligatory trigger for the upregulation of serine palmitoyltransferase mRNA expression in human epidermis PMID: 12445191
7. Results suggest that functional serine palmitoyltransferase is not a dimer, but a higher organized complex, composed of three distinct subunits (SPTLC1, SPTLC2 and SPTLC3) with a molecular mass of 480 kDa. PMID: 17331073
8. discovery of 2 proteins, ssSPTa and ssSPTb, which each interacts with both hLCB1 and hLCB2, suggesting that there are 4 distinct human SPT isozymes. PMID: 19416851
顯示更多

收起更多
相關疾病：

Neuropathy, hereditary sensory and autonomic, 1C (HSAN1C)
亞細胞定位：

Endoplasmic reticulum membrane; Single-pass membrane protein.
蛋白家族：

Class-II pyridoxal-phosphate-dependent aminotransferase family
組織特異性：

Widely expressed.
數據庫鏈接：

HGNC: 11278

OMIM: 605713

KEGG: hsa:9517

STRING: 9606.ENSP00000216484

UniGene: Hs.435661

Most popular with customers

YWHAG Recombinant Monoclonal Antibody

Applications: ELISA, WB, IHC

Species Reactivity: Human
Phospho-YAP1 (S127) Recombinant Monoclonal Antibody

Applications: ELISA, WB, IHC

Species Reactivity: Human
XRCC5 Recombinant Monoclonal Antibody

Applications: ELISA, WB, IHC, IF

Species Reactivity: Human
XPNPEP1 Recombinant Monoclonal Antibody

Applications: ELISA, IHC, FC

Species Reactivity: Human
XDH Recombinant Monoclonal Antibody

Applications: ELISA, WB, IHC

Species Reactivity: Human
WFDC2 Recombinant Monoclonal Antibody

Applications: ELISA, WB, FC

Species Reactivity: Human
WDR5 Recombinant Monoclonal Antibody

Applications: ELISA, IHC, FC

Species Reactivity: Human
VIM Recombinant Monoclonal Antibody

Applications: ELISA, IHC, IF

Species Reactivity: Human