Phospho-HIST1H1B (S17) Antibody

說明書

中文名稱：

磷酸化-HIST1H1B (S17)兔多克隆抗體
貨號：

CSB-PA010377PA17phHU
規格：

￥660
圖片：
Western Blot
Positive WB detected in: MCF-7 whole cell lysate
All lanes: HIST1H1B antibody at 1µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 23 kDa
Observed band size: 32 kDa

Immunocytochemistry analysis of HepG2 cells using CSB-PA010377PA17phHU at dilution of 1:100

Immunofluorescence staining of HepG2 cells with CSB-PA010377PA17phHU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
其他：

產品詳情
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靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) HIST1H1B Polyclonal antibody
Uniprot No.：

P16401
基因名：

HIST1H1B
別名：

H1 antibody; H1 histone family member 5 antibody; H1.5 antibody; H15 HUMAN antibody; H15_HUMAN antibody; H1B antibody; H1F5 antibody; H1s 3 antibody; Hist1h1b antibody; Histone 1 H1b antibody; Histone cluster 1 H1b antibody; Histone H1.5 antibody; Histone H1a antibody; Histone H1b antibody; Histone H1s 3 antibody; MGC126630 antibody; MGC126632 antibody
宿主：

Rabbit
反應種屬：

Human
免疫原：

Peptide sequence around site of Phospho-Ser (17) derived from Human Histone H1.5
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

Antigen Affinity Purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, WB, ICC, IF

推薦稀釋比：

Application	Recommended Dilution
WB	1:100-1:1000
ICC	1:20-1:200
IF	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation.
基因功能參考文獻：
1. Data indicate that normal ovarian tissues strongly expressed histone H1.5, whereas ovarian granulosa cell tumors (GCTs) weakly expressed this protein; in contrast, PLZF protein expression was not significantly different between both study groups. PMID: 25023763
2. Statistically significant differences in staining patterns were found for histone H1.5, distinguishing leiomyosarcomas from leiomyomas. PMID: 24784718
3. Promyelocytic leukemia zinc finger and histone H1.5 differentially stain low- and high-grade pulmonary neuroendocrine tumors PMID: 23416030
4. FoxP3 interacts with H1.5 to alter its binding to target genes to modulate their expression and to program Treg function. PMID: 21654845
5. the mode of the chromatin fiber compaction changes depending both on salt environment and linker histone H1 PMID: 16185066
6. Phosphorylation of human H1 variants occurs nonrandomly during both interphase and mitosis and distinct serine- or threonine-specific kinases are involved in different cell cycle phases. PMID: 16377619
7. Phosphorylation at threonine 10 appears in prometaphase and disappears in telophase, and that this hyperphosphorylated form of H1.5 is mainly chromatin-bound in metaphase when chromatin condensation is maximal. PMID: 19136008
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亞細胞定位：

Nucleus. Chromosome. Note=According to PubMed:15911621 more commonly found in heterochromatin. According to PubMed:10997781 associates with actively transcribed chromatin and not heterochromatin.
蛋白家族：

Histone H1/H5 family
組織特異性：

Ubiquitous. Expressed in the majority of the cell lines tested and in testis.
數據庫鏈接：

HGNC: 4719

OMIM: 142711

KEGG: hsa:3009

STRING: 9606.ENSP00000330074

UniGene: Hs.131956

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