Phospho-FOS (Thr232) Antibody

說明書

中文名稱：

磷酸化-FOS (Thr232)兔多克隆抗體
貨號：

CSB-PA595307
規(guī)格：

￥2454
圖片：
Western blot analysis of extracts from COS7 cells (Lane 2), using FOS (Phospho-Thr232) Antibody. The lane on the left is treated with antigen-specific peptide.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using FOS (Phospho-Thr232) antibody (left)or the same antibody preincubated with blocking peptide (right).
其他：

產(chǎn)品詳情
產(chǎn)品評價
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靶點詳情

產(chǎn)品詳情

產(chǎn)品名稱：

Rabbit anti-Homo sapiens (Human) FOS Polyclonal antibody
Uniprot No.：

P01100
基因名：

FOS
宿主：

Rabbit
反應(yīng)種屬：

Human,Mouse
免疫原：

Peptide sequence around phosphorylation site of threonine 232(V-A-T(p)-P-E) derived from Human FOS .
免疫原種屬：

Homo sapiens (Human)
克隆類型：

Polyclonal
純化方式：

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
濃度：

It differs from different batches. Please contact us to confirm it.
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA,WB,IHC

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:1000
IHC	1:50-1:100

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點詳情

功能：

Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. In growing cells, activates phospholipid synthesis, possibly by activating CDS1 and PI4K2A. This activity requires Tyr-dephosphorylation and association with the endoplasmic reticulum.
基因功能參考文獻(xiàn)：
1. Findings iindicate a human bone tumour defined by mutations of FOS and FOSB. PMID: 29858576
2. gammadelta T cells suppressed iDCs osteoclastogenesis by downregulation of the RANK/cFos/ATP6V0D2 signaling pathway. PMID: 30066839
3. Mutant cellular AP-1 proteins promote expression of a subset of Epstein-Barr virus late genes in the absence of lytic viral DNA replication. PMID: 30021895
4. Low c-fos expression is associated with Oral Squamous Cell Carcinoma. PMID: 29582647
5. Study demonstrated that c-Fos was highly expressed in most of ovarian epithelial carcinoma cases and was significantly correlated with Lewis y. Also, the results revealed that c-Fos interacted with the FUT1 promoter. Silencing of c-Fos prevented TGF-beta1-induced Lewis y expression. PMID: 29130097
6. These findings indicate that the c-Fos/miR-22/MDC1 axis plays a relevant role in DNA repair in terminally differentiated cells, which may facilitate our understanding of molecular mechanism underlying the downregulating DNA repair in differentiated cells. PMID: 28637007
7. our results strongly suggest a novel role of c-Fos as a regulator of epithelial-mesenchymal transition and cancer stem cell(CSC) reprogramming in Head and neck squamous cell carcinoma (HNSCC)cells, which may hold potential as a CSC-directed therapeutic approach to improve HNSCC treatment PMID: 27965308
8. High c-fos expression is associated with malignant glioma. PMID: 27602752
9. Immunohistochemistry was employed to analyze cFos, cJun and CD147 expression in 41 UCB cases and 34 noncancerous human bladder tissues. PMID: 28358415
10. data enforced the evidence that knockdown of c-Fos inhibited cell proliferation, migration, and invasion, and promoted the apoptosis of OS cells accompanied by altered expression of Wnt2 and Fzd9 PMID: 28665975
11. These findings demonstrate an essential role for the ERK pathway together with c-JUN and c-FOS in the differentiation activity of LukS-PV. PMID: 27102414
12. novel function of KDM2B in the negative regulation of cell proliferation by assembling an E3 ligase to targeting c-Fos protein degradation that is antagonized by mitogenic stimulations PMID: 26725323
13. NF-Y Binding Site Architecture Defines a C-Fos Targeted Promoter Class PMID: 27517874
14. c-fos underexpression is associated with Myelodysplastic Syndrome. PMID: 27513856
15. miR-101 is downregulated in bladder cancer cells and has an inhibitory role in the regulation of bladder cancer cell proliferation and invasion via directly targeting c-FOS. PMID: 27485165
16. We found that c-jun or c-fos was significantly associated with lymph node metastasis, and coexpression of c-jun/c-fos, or c-jun/c-fos/p53 were significantly associated with lymph node metastasis, poor differentiation and clinical stage. PMID: 27558649
17. CRAC channel blockade also suppressed Oxo-M-induced c-fos and interleukin-2 expression PMID: 27474128
18. The results indicate that 17beta-estradiol-induced endometrial stromal cell invasion is dependent on c-fos-mediated MMP-9 expression. PMID: 26917263
19. FOS is a downstream effector of high glucose stimulation in peritoneal mesothelial cells that contributes to TGF-beta1 production. PMID: 26018137
20. VEGF-induced endothelial migration is mediated primarily by induction of JunB whereas the promotion of endothelial proliferation by VEGF is mediated by JunB-independent AP-1 family members. PMID: 26860974
21. c-Fos can protect against HDAC3 neurotoxicity. PMID: 25592718
22. These results indicate that IL-17A enhances COX2 expression and PGE2 production via the p38/c-Fos and JNK/c-Jun signalling pathways in NP cells to mediate intervertebral disc inflammation. PMID: 26988982
23. the results of this study suggest that FOS is among the candidate genes of schizophrenia and that changes in the expression of c-Fos protein may contribute to molecular mechanisms of schizophrenia-related alterations in synaptic plasticity. PMID: 25706621
24. Increased c-Fos expression is through TRPM3-mediated stimulation of the c-Fos promoter. PMID: 26493679
25. A novel AP-1 binding site at -1363 bp of the human TF promoter region was identified. PMID: 26631725
26. Simultaneous high expression of ID1 and c-Jun or c-Fos was correlated with poor survival in esophageal squamous cell carcinoma patients. PMID: 26858249
27. miR-146a has a role in targeting Fos expression in human cardiac cells PMID: 26112171
28. The translocation causes truncation of the FOS protein, with loss of the transactivation domain, which is thereby a novel mechanism involved in tumorigenesis. PMID: 26173738
29. ERK1 and ERK2 regulated the expression of c-Fos and c-Jun proteins in human cervical cancer cells. PMID: 25647783
30. O-GlcNAcylation of MLL5beta at T440 residue is critical for MLL5 recruitment to the HPV16/18-long control region through its interaction with AP-1. PMID: 25670814
31. The RNA binding complexes NF45-NF90 and NF45-NF110 associate dynamically with the c-fos gene and function as transcriptional coactivators. PMID: 26381409
32. Data show that interleukin-1 receptor type 2 (IL1R2) forms a complex with c-Fos proto-oncogene protein and activates the interleukin-6 (IL-6) and vascular endothelial growth factor A (VEGF-A) promoters. PMID: 26209639
33. Data indicate that deregulation of transcription factor AP-1 and microRNA-21-mediated axis led to an enhanced cell growth in hepatocellular carcinoma (HCC). PMID: 25544773
34. These results establish c-Fos homodimers as a novel form of the AP-1 complex that may be an autonomous transcription factor in c-Fos-overexpressing tissues and could contribute to tumor development. PMID: 26303532
35. Endoplasmic reticulum stress activates the hepatic AP-1 complex via MAPK-dependent signaling pathways. PMID: 25077945
36. co-expression of c-Fos or Fra1 was able to cooperate with TAp73 in potentiating cellular growth, similarly to c-Jun. These data together suggest that TAp73 plays a vital role in activation of AP-1 target genes via direct binding to c-Jun PMID: 26018080
37. The light-induced FOS response in melanopsin expressing HEK-293 cells is correlated with melanopsin quantity and dependent on light duration and irradiance. PMID: 24909488
38. c-Fos promotes the progression of viral transcription from early to late stages and accelerates viral lytic replication upon sustained ORF45-ERK-RSK activation during the Kaposi's Sarcoma-Associated Herpesvirus lytic life cycle. PMID: 25903346
39. By targeting the proto-oncogene Fos, miR-101 is involved in G1-to-S phase transition in cervical cancer cells in vitro. PMID: 24987920
40. Data suggest that p38 MAP kinase regulates c-Fos/cellular oncogene fos mRNA stability/decay by affecting state of phosphorylation of ELAVL1/HuR (Hu antigen R). PMID: 25588078
41. CDK12 plays an important role in cotranscriptional processing of c-FOS transcripts PMID: 25384976
42. We found significant negative correlations regarding the expression of the genes COMT, MAOB, DRD4, DRD5 and FOS, indicating that increased schizotypy coincides with higher levels of dopaminergic dysregulation on the mRNA-level. PMID: 24630741
43. results support the proposal that cooperative signaling of both NF-kappaB and AP1 (via p38alpha) amplifies STIM1 expression in ECs and, thereby, contributes to the lung vascular hyperpermeability response during sepsis PMID: 25016017
44. SMAR1 has a role in repressing c-Fos-mediated HPV18 E6 transcription through alteration of chromatin histone deacetylation PMID: 25157104
45. This study indicates that increased expression of c-Fos, p-c-Jun, members of AP-1 transcriptional factor and p-JNK is associated with neuronal degeneration in the ganglion cell layer of retinas in diabetic patients. PMID: 24073601
46. S100A4, FOS and CXCR4, playing a major role in tumor progression and metastasis, are downregulated by sorafenib. PMID: 24378831
47. the IL-1beta/p38/AP-1(c-fos)/MMP2 & MMP9 pathway play an important role in metastasis in gastric adenocarcinoma PMID: 24479681
48. the distinct requirement of NF-kappaB for mouse and human c-fos regulation PMID: 24386331
49. c-Fos, a well known AP-1 transcription factor, has emerged as a unique protein with the capacity to associate to specific enzymes of the pathway of synthesis of phospholipids at the endoplasmic reticulum and activate their synthesis. (Review) PMID: 24886961
50. Inflammation mediators act through c-Fos to increase VEGF production in peritoneal mesothelium. PMID: 23760290
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亞細(xì)胞定位：

Nucleus. Endoplasmic reticulum. Cytoplasm, cytosol. Note=In quiescent cells, present in very small amounts in the cytosol. Following induction of cell growth, first localizes to the endoplasmic reticulum and only later to the nucleus. Localization at the endoplasmic reticulum requires dephosphorylation at Tyr-10 and Tyr-30.
蛋白家族：

BZIP family, Fos subfamily
數(shù)據(jù)庫鏈接：

HGNC: 3796

OMIM: 164810

KEGG: hsa:2353

STRING: 9606.ENSP00000306245

UniGene: Hs.25647

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