PPM1K Antibody

說明書

中文名稱：

PPM1K兔多克隆抗體
貨號：

CSB-PA822697LA01HU
規格：

￥3,900
圖片：
Western Blot
Positive WB detected in: COLO205 whole cell lysate(30μg), 293 whole cell lysate(30μg), A549 whole cell lysate(30μg), JK whole cell lysate(30μg), K562 whole cell lysate(30μg), Hela whole cell lysate(30μg),Mouse skeletal muscle tissue lysate(30μg)
All lanes: PPM1K antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/20000 dilution
Predicted band size: 41,26,17 kDa
Observed band size: 41 kDa
Exposure time: 120s

IHC image of CSB-PA822697LA01HU diluted at 1:100 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody

IHC image of CSB-PA822697LA01HU diluted at 1:100 and staining in paraffin-embedded human colorecta cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
其他：

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靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) PPM1K Polyclonal antibody
Uniprot No.：

Q8N3J5
基因名：

PPM1K
別名：

BDP antibody; mitochondrial antibody; MSUDMV antibody; PP2C domain containing protein phosphatase 1K antibody; PP2C domain-containing protein phosphatase 1K antibody; PP2C kappa antibody; PP2C like mitochondrial protein antibody; PP2C type mitochondrial phosphoprotein phosphatase antibody; PP2C-kappa antibody; PP2C-like mitochondrial protein antibody; PP2C-type mitochondrial phosphoprotein phosphatase antibody; PP2Ckappa antibody; PP2CM antibody; PPM1K antibody; PPM1K_HUMAN antibody; Protein phosphatase 1K antibody; Protein phosphatase 1K; mitochondrial antibody; Protein phosphatase 2C isoform kappa antibody; Protein phosphatase Mg2+/Mn2+ dependent 1K antibody; PTMP antibody; UG0882E07 antibody
宿主：

Rabbit
反應種屬：

Human, Mouse
免疫原：

Recombinant Human Protein phosphatase 1K, mitochondrial protein (30-372AA)
免疫原種屬：

Homo sapiens (Human)
克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

Antigen?affinity?purification
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.02% sodium azide
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:100-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Regulates the mitochondrial permeability transition pore and is essential for cellular survival and development.
基因功能參考文獻：
1. Branched chain amino acids related genetic variant rs1440581 was associated with an increased risk of incident T2 Diabetes in a Chinese population. This variant might modify effect of weight gain on development in Insulin resistance. PMID: 29855804
2. PPM1K rs1440581 may affect changes in glucose metabolism during weight loss, and this effect is dependent on dietary fat and carbohydrate intakes. PMID: 28768654
3. miR-204 and miR-211 directly repress PPM1K expression via 3'UTR. PMID: 26592513
4. Overweight people with the C allele of the branched-chain amino acid/aromatic amino acid ratio-associated variant rs1440581 may benefit less in weight loss and improved insulin sensitivity than those without it on a energy-restricted high-fat diet. PMID: 23446828
5. The 2.4 A PP2Cm structure reveals a central beta-sandwich with two bound metal ions in the active-site cleft. PMID: 22291014
6. A novel member of human protein phosphatase 2C gene family named PP2Ckappa was isolated from a human fetal brain cDNA library. PMID: 16685424
7. The cloned phosphatase has a mitochondrial leader sequence and its amino acid sequence places it in the PP2C family like two known mitochondrial phosphatases. PMID: 17336929
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相關疾病：

Maple syrup urine disease, mild variant (MSUDMV)
亞細胞定位：

Mitochondrion matrix.
蛋白家族：

PP2C family
數據庫鏈接：

HGNC: 25415

OMIM: 611065

KEGG: hsa:152926

STRING: 9606.ENSP00000295908

UniGene: Hs.43744

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