PPM1F Antibody
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中文名稱：PPM1F兔多克隆抗體
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貨號：CSB-PA018493LA01HU
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規(guī)格：￥440
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圖片：
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Western Blot
Positive WB detected in: Mouse liver tissue
All lanes: PPM1F antibody at 3μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 50, 39 kDa
Observed band size: 50 kDa -
IHC image of CSB-PA018493LA01HU diluted at 1:100 and staining in paraffin-embedded human melanoma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. -
IHC image of CSB-PA018493LA01HU diluted at 1:100 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. -
Immunofluorescence staining of HepG2 cells with CSB-PA018493LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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其他：
產(chǎn)品詳情
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產(chǎn)品名稱：Rabbit anti-Homo sapiens (Human) PPM1F Polyclonal antibody
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Uniprot No.：
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基因名：PPM1F
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別名：Ca(2+)/calmodulin-dependent protein kinase phosphatase antibody; CaM-kinase phosphatase antibody; CAMKP antibody; CaMKPase antibody; FEM 2 antibody; hFEM-2 antibody; KIAA0015 antibody; Partner of PIX 2 antibody; POPX2 antibody; PP2C phosphatase antibody; Ppm1f antibody; PPM1F_HUMAN antibody; Protein fem-2 homolog antibody; Protein phosphatase 1F (PP2C domain containing) antibody; Protein phosphatase 1F antibody; Protein phosphatase Mg2+/Mn2+ dependent 1F antibody
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宿主：Rabbit
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反應種屬：Human, Mouse
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免疫原：Recombinant Human Protein phosphatase 1F protein (1-76AA)
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免疫原種屬：Homo sapiens (Human)
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標記方式：Non-conjugated
本頁面中的產(chǎn)品，PPM1F Antibody (CSB-PA018493LA01HU)，的標記方式是Non-conjugated。對于PPM1F Antibody，我們還提供其他標記。見下表：
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克隆類型：Polyclonal
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抗體亞型：IgG
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純化方式：>95%, Protein G purified
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 -
產(chǎn)品提供形式：Liquid
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應用范圍：ELISA, WB, IHC, IF
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推薦稀釋比：
Application Recommended Dilution WB 1:500-1:5000 IHC 1:20-1:200 IF 1:50-1:200 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產(chǎn)品評價
相關產(chǎn)品
靶點詳情
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功能：Dephosphorylates and concomitantly deactivates CaM-kinase II activated upon autophosphorylation, and CaM-kinases IV and I activated upon phosphorylation by CaM-kinase kinase. Promotes apoptosis.
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基因功能參考文獻：
- PPM1F overexpression suppressed cell proliferation and invasion and counteracted the tumour-promoting role of miR-590, but PPM1F knockdown reversed these effects. PMID: 29473240
- POPX2 is a negative regulator of TAK1 signaling pathway and modulates apoptosis through the regulation of TAK1 activity. PMID: 28906490
- PPM1F could work downstream of alpha9-nAchR to promote nicotine-induced carcinogenic signals. Thus, PPM1F expression could be used for prognostic diagnosis, or inhibited as a potential strategy for cancer prevention and therapy. PMID: 27769050
- This study, combined with our previous findings, suggests that a single ubiquitously expressed phosphatase POPX2 influences cancer metastasis via modulating multiple biological processes including MAPK signaling and exosome cytokine secretion. PMID: 27976581
- Overexpression of PPM1F is associated with metastasis of hepatocellular carcinoma. PMID: 26498692
- POPX2 affects trafficking by determining the phosphorylation status of KIF3A at serine 690. PMID: 24338362
- POPX2 might regulate cell motility through its regulation of the MAPK1/3, leading to changes in the cytoskeleton and cell motility. PMID: 23621870
- hCaMKP activity is reversibly regulated by oxidation/reduction at Cys-359 PMID: 22743349
- FHOD1 and PPM1F (direct regulators of the actin cytoskeleton) were identified as novel targets of miR-200c. Expression levels of FHOD1 and PPM1F were inversely correlated with miR-200c level in breast cancer cell lines and breast cancer patient samples. PMID: 22144583
- Data identify a biochemical pathway through which POPX2 exerts its apparent cellular function: the regulation of activity of glycogen synthase kinase-3, which in turn modulates extracellular signal-regulated kinase and cell motility. PMID: 21656682
- The POPX2 phosphatase regulates cancer cell motility and invasiveness. PMID: 20016286
- The p21-activated kinase PAK is negatively regulated by POPX1 and POPX2, a pair of serine/threonine phosphatases of the PP2C family. (POPX1; POPX2) PMID: 11864573
- PPM1F plays a key role in the regulation of CaMKII intracellularly PMID: 15140879
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蛋白家族：PP2C family
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