POLM Antibody
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中文名稱：POLM兔多克隆抗體
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貨號：CSB-PA004757
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規格：￥1090
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圖片：
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Western Blot analysis of K562 cells using DNA Pol 籠Polyclonal Antibody
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其他：
產品詳情
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Uniprot No.：
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基因名：POLM
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別名：DNA-directed DNA polymerase mu antibody; DNA-directed DNA/RNA polymerase mu antibody; DPOLM_HUMAN antibody; FLJ35482 antibody; Pol iota antibody; Pol Mu antibody; Polm antibody; POLM protein antibody; Polymerase (DNA directed) mu antibody; Polymerase DNA directed mu antibody; Tdt N antibody; Tdt-N antibody; TdtN antibody; Terminal transferase antibody
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宿主：Rabbit
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反應種屬：Human
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免疫原：Synthesized peptide derived from the Internal region of Human DNA Pol μ.
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免疫原種屬：Homo sapiens (Human)
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標記方式：Non-conjugated
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抗體亞型：IgG
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純化方式：The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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產品提供形式：Liquid
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應用范圍：WB, ELISA
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推薦稀釋比：
Application Recommended Dilution WB 1:500-1:2000 ELISA 1:40000 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產品評價
相關產品
靶點詳情
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功能：Gap-filling polymerase involved in repair of DNA double-strand breaks by non-homologous end joining (NHEJ). Participates in immunoglobulin (Ig) light chain gene rearrangement in V(D)J recombination.
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基因功能參考文獻：
- Polmu point mutations affecting 2 conserved adjacent residues located in the 8 kDa domain, G174S and R175H, limit the efficiency of accurate NHEJ by Polmu in vitro and in vivo due to decreased template dependency during NHEJ, which renders the error-rate of the mutants higher due to the ability of Polmu to randomly incorporate nucleotides at DSBs. PMID: 28973441
- Structural accommodation of ribonucleotide incorporation by the DNA repair enzyme polymerase Mu has been described. PMID: 28911097
- analysis of template-dependent synthesis by human polymerase mu PMID: 26240373
- A study of how Polmu fixes and/or orients the mobile Loop1 part of the protein in accordance with the substrate on which it is polymerizing. PMID: 24878922
- specific loop 1 residues contribute to Pol mu's unique ability to catalyze template-dependent NHEJ of DSBs with unpaired 3' ends PMID: 24487959
- evidence suggests that Polmu could be regulated in vivo by phosphorylation of the BRCT domain (Ser12/Thr21) and of Ser372, affecting the function of loop1; Polmu's most distinctive activities would be turned off at specific cell-cycle phases (S and G2), when these functions might be harmful to the cell PMID: 23933132
- A specific N-terminal extension of the 8 kDa domain of DNA polymerase mu is potentially implicated in the maintenance of a closed conformation throughout the catalytic cycle, and this study indicated that it could be a target of Cdk phosphorylation. PMID: 23935073
- A physiological concentration of Mn(2+) ions did benefit Polmicro-mediated non-homologous end joining by improving the efficiency and accuracy of nucleotide insertion. PMID: 23275568
- The study points at human Polmicro residues His(329) and Arg(387) as responsible for regulating nucleotide expansions occurring during DNA repair transactions, either promoting or blocking, respectively, iterative polymerization. PMID: 23143108
- The results uncovered a new DNA-binding function for the BRCT domain of Polmicro and demonstrated the importance of several residues located at the primer-binding region, for both DNA-binding and polymerization activities. PMID: 23034807
- Pol mu binds to DNA through its amino-terminal and pol beta-like regions. PMID: 22897684
- DNA polymerase mu performs DNA synthesis at a AAF lesion PMID: 11972346
- Association of DNA polymerase mu (pol mu) with Ku and ligase IV: role for pol mu in end-joining double-strand break repair. PMID: 12077346
- DNA polymerase mu acts in response to several types of DNA damage with a lesion bypass mechanism PMID: 12228225
- expression in B-cell non-Hodgkin's lymphomas PMID: 12368208
- Pol mu's substrate specificity is similar to that of pol beta in most respects but has an approximately 1,000-fold-reduced ability to discriminate against ribonucleotides compared to pol beta PMID: 12640116
- human DNA polymerase mu has a template-dependent, sequence-independent nucleotidyl transferase activity PMID: 14581466
- Poliota incorporates a C opposite the gamma-HOPdG adduct with nearly the same efficiency as opposite a nonadducted G residue. The subsequent extension step is performed by Polkappa, which efficiently extends from the C incorporated opposite the adduct. PMID: 15199127
- DNA polymerase mu has been overexpressed, purified, and its fidelity estimated for incorporation of both deoxynucleotides and ribonucleotides based on pre-steady-state kinetic data under single-turnover conditions. PMID: 15504045
- Overexpression of DNA polymerase mu in a Burkitt's lymphoma cell line induced an increase in somatic mutations specifically targeted to G/C residues in immunoglobulin variable genes. PMID: 15520469
- Pol mu promotes accuracy during Ig kappa recombination. PMID: 16061182
- When the terminal deoxynucleotidyl transferase (TdT) loop1 was deleted, human Polmu lacked TdT activity but improved DNA-binding and DNA template-dependent polymerization. PMID: 16963491
- Studies shed light on the mechanism by which a rate-limited terminal transferase activity in Polmu could regulate the balance between accuracy and necessary efficiency, providing some variability during NHEJ. PMID: 19805281
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亞細胞定位：Nucleus.
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蛋白家族：DNA polymerase type-X family
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組織特異性：Expressed in a number of tissues. Abundant in thymus.
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數據庫鏈接：
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