PIR Antibody

中文名稱：

PIR兔多克隆抗體
貨號：

CSB-PA018032LA01HU
規(guī)格：

￥440
圖片：
Immunohistochemistry of paraffin-embedded human liver tissue using CSB-PA018032LA01HU at dilution of 1:100

Immunofluorescence staining of Hela cells with CSB-PA018032LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of U251 cells with CSB-PA018032LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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產(chǎn)品詳情
產(chǎn)品評價(jià)
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靶點(diǎn)詳情

產(chǎn)品詳情

產(chǎn)品名稱：

Rabbit anti-Homo sapiens (Human) PIR Polyclonal antibody
Uniprot No.：

O00625
基因名：

PIR
別名：

3-dioxygenase PIR antibody; Iron binding nuclear protein antibody; Pir antibody; PIR_HUMAN antibody; Pirin antibody; Probable quercetin 2 antibody; probable quercetin 2,3-dioxygenase PIR antibody; Probable quercetinase antibody
宿主：

Rabbit
反應(yīng)種屬：

Human
免疫原：

Recombinant Human Pirin protein (1-290AA)
免疫原種屬：

Homo sapiens (Human)

標(biāo)記方式：

Non-conjugated

本頁面中的產(chǎn)品，PIR Antibody (CSB-PA018032LA01HU)，的標(biāo)記方式是Non-conjugated。對于PIR Antibody，我們還提供其他標(biāo)記。見下表：

標(biāo)記方式	貨號	產(chǎn)品名稱	應(yīng)用
HRP	CSB-PA018032LB01HU	PIR Antibody, HRP conjugated	ELISA
FITC	CSB-PA018032LC01HU	PIR Antibody, FITC conjugated
Biotin	CSB-PA018032LD01HU	PIR Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, IHC, IF

推薦稀釋比：

Application	Recommended Dilution
IHC	1:20-1:200
IF	1:50-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲(chǔ)存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價(jià)

靶點(diǎn)詳情

功能：

Transcriptional coregulator of NF-kappa-B which facilitates binding of NF-kappa-B proteins to target kappa-B genes in a redox-state-dependent manner. May be required for efficient terminal myeloid maturation of hematopoietic cells. Has quercetin 2,3-dioxygenase activity (in vitro).
基因功能參考文獻(xiàn)：
1. Data suggest that only the Fe(III) form of pirin [not the Fe(II) form] enhances affinity of binding between p65 and DNA in the pirin-p65-DNA supramolecular complex. (pirin = quercetin 2,3-dioxygenase; p65 = RELA proto-oncogene NF-kappaB subunit) PMID: 28825294
2. Pirin regulates E-cadherin independently of Bcl3-Slug signaling. PMID: 25680527
3. The NRF2 transcription factor binds to this element in vivo and drives the basal PIR expression. PMID: 24390086
4. Pirin is a newly identified binding partner of EAF2/U19 capable of down-regulating EAF2/U19 protein and alleviating its inhibition of prostate cancer cell survival/proliferation. PMID: 24272884
5. Data indicate that the R-shaped area composes the interface for pirin-NF-kappaB binding that is responsible for modulation of NF-kappaB's DNA-binding properties. PMID: 23716661
6. pirin may have a relevant role in melanoma progression. PMID: 21514450
7. knockdown of pirin or treatment with the small molecule inhibited melanoma cell migration PMID: 20711196
8. This study aimed to examine genetic variations in the PIR gene by a comprehensive tagging method and its sex-specific effects on bone mineral density and osteoporotic risk. PMID: 19766747
9. An abnormal pattern of PIR sub-cellular localization is a characteristic feature of a subset of melanomas, and suggest it may represent a marker associated with disease progression. PMID: 20089166
10. Pirin downregulation is a feature of acute myeloid leukemia and leads to impairment of terminal myeloid differentiation. PMID: 20010624
11. x-ray crystallography of of human pirin shows that it is an iron-binding nuclear protein and transcription cofactor PMID: 14573596
12. First report of enzymatic activity for any member of the Pirin family which may be an important connection to their roles in transcriptional regulation. PMID: 15951572
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亞細(xì)胞定位：

Nucleus. Cytoplasm.
蛋白家族：

Pirin family
組織特異性：

Highly expressed in a subset of melanomas. Detected at very low levels in most tissues (at protein level). Expressed in all tissues, with highest level of expression in heart and liver.
數(shù)據(jù)庫鏈接：

HGNC: 30048

OMIM: 300931

KEGG: hsa:8544

STRING: 9606.ENSP00000369785

UniGene: Hs.495728

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