PACSIN2 Antibody
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中文名稱：PACSIN2兔多克隆抗體
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貨號：CSB-PA890765EA01HU
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規(guī)格：￥440
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圖片：
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IHC image of CSB-PA890765EA01HU diluted at 1:400 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. -
IHC image of CSB-PA890765EA01HU diluted at 1:400 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. -
Immunofluorescence staining of Hela cells with CSB-PA890765EA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). -
Western Blot
Positive WB detected in: 293T whole cell lysate, HepG2 whole cell lysate, HL60 whole cell lysate
All lanes: PACSIN2 antibody at 2.6μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 56, 52 kDa
Observed band size: 56 kDa -
Immunoprecipitating PACSIN2 in HepG2 whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA890765EA01HU in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA890765EA01HU (6μg) + HepG2 whole cell lysate (500μg)
Lane 3: HepG2 whole cell lysate (20μg)
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其他：
產(chǎn)品詳情
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產(chǎn)品名稱：Rabbit anti-Homo sapiens (Human) PACSIN2 Polyclonal antibody
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Uniprot No.：
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基因名：PACSIN2
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別名：cytoplasmic phosphoprotein PACSIN2 antibody; OTTHUMP00000198281 antibody; OTTHUMP00000198282 antibody; OTTHUMP00000198283 antibody; OTTHUMP00000198284 antibody; PACN2_HUMAN antibody; Pacsin2 antibody; protein kinase C and casein kinase substrate in neurons 2 antibody; Protein kinase C and casein kinase substrate in neurons protein 2 antibody; SDPII antibody; Syndapin II antibody
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宿主：Rabbit
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反應(yīng)種屬：Human
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免疫原：Recombinant Human Protein kinase C and casein kinase substrate in neurons protein 2 protein (240-410AA)
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免疫原種屬：Homo sapiens (Human)
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標記方式：Non-conjugated
本頁面中的產(chǎn)品，PACSIN2 Antibody (CSB-PA890765EA01HU)，的標記方式是Non-conjugated。對于PACSIN2 Antibody，我們還提供其他標記。見下表：
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克隆類型：Polyclonal
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抗體亞型：IgG
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純化方式：>95%, Protein G purified
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 -
產(chǎn)品提供形式：Liquid
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應(yīng)用范圍：ELISA, WB, IHC, IF, IP
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推薦稀釋比：
Application Recommended Dilution WB 1:500-1:5000 IHC 1:200-1:500 IF 1:50-1:200 IP 1:200-1:2000 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點詳情
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功能：Regulates the morphogenesis and endocytosis of caveolae. Lipid-binding protein that is able to promote the tubulation of the phosphatidic acid-containing membranes it preferentially binds. Plays a role in intracellular vesicle-mediated transport. Involved in the endocytosis of cell-surface receptors like the EGF receptor, contributing to its internalization in the absence of EGF stimulus.; (Microbial infection) Specifically enhances the efficiency of HIV-1 virion spread by cell-to-cell transfer. Also promotes the protrusion engulfment during cell-to-cell spread of bacterial pathogens like Listeria monocytogenes. Involved in lipid droplet formation, which is important for HCV virion assembly.
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基因功能參考文獻：
- Pacsin2 recruitment is important for maintenance of cell-cell adhesion by stabilizing the VE-cadherin complex within focal adherens junctions and inhibition of its internalization. PMID: 27417273
- observations indicate that PACSIN2 promotes the cell-to-cell spreading of HIV-1 by connecting Gag to the actin cytoskeleton. PMID: 29891700
- To our knowledge, this is the first study showing PACSIN2 genotype association with hematological toxicity in ALL patients undergoing maintenance therapy. PMID: 27452984
- FlnA binding to PACSIN2 F-BAR domain regulates membrane tubulation in megakaryocytes and platelets. PMID: 25838348
- our results provide new insights into the mechanism of cellular intoxication by TcdA and the role of PACSIN2 in endocytosis. PMID: 27942025
- proposed a packing model for the assembly of PACSIN 2 on membrane, where the proteins are connected by tip-to-tip and wedge loop-mediated lateral interactions on the surface of membrane to generate various diameter tubules PMID: 23888307
- PACSIN2 phosphorylation decreases its membrane-binding activity, thereby decreasing its stabilizing effect on caveolae and triggering dynamin-mediated removal of caveolae. PMID: 26092940
- PC1 modulates actin cytoskeleton rearrangements and directional cell migration through the Pacsin 2/N-Wasp complex. PMID: 24385601
- Cooperation of MICAL-L1, pacsin 2 (syndapin2), and phosphatidic acid in tubular recycling endosome biogenesis. PMID: 23596323
- These data indicate that polymorphism in PACSIN2 significantly modulates TPMT activity and influences the risk of GI toxicity associated with mercaptopurine therapy. PMID: 22846425
- a novel role for the F-BAR-domain protein PACSIN2 in regulating EGF receptor surface levels and EGF-induced downstream signaling. PMID: 23129763
- important role in the formation of plasma membrane caveolae PMID: 21807942
- Data identify the BAR-domain protein PACSIN2 as a Rac1 interactor that regulates Rac1-mediated cell spreading and migration. PMID: 21693584
- PACSIN2 regulates cell spreading and cell migration, which are dependent on cyclin D1 expression. PMID: 21200149
- Results indicate that PACSIN2 mediates membrane sculpting by caveolin-1 in caveola morphology and recruits dynamin-2 for caveola fission. PMID: 21610094
- Since the curvature of the neck of the microspike and that of the tubulation share similar geometry, the pacsin2 EFC/F-BAR domain is considered to facilitate both microspike formation and tubulation. PMID: 20188097
- Thus, syndapin-dynamin complexes are crucial and sufficient to promote vesicle formation from the trans-Golgi network. PMID: 16551695
- These findings suggest a novel function for PACSIN proteins 1, 2, and 3 in dynamic microtubule nucleation. PMID: 18456257
- The regulated deformation of membranes and promotion of tubule constrictions by Pacsin suggests a more versatile function of these proteins in vesiculation and endocytosis. PMID: 19549836
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亞細胞定位：Cytoplasm. Cytoplasm, cytoskeleton. Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Early endosome. Recycling endosome membrane. Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection. Membrane, caveola. Note=Detected at the neck of flask-shaped caveolae. Localization to tubular recycling endosomes probably requires interaction with MICALL1 and EHD1.
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蛋白家族：PACSIN family
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組織特異性：Widely expressed.
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數(shù)據(jù)庫鏈接：
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