NIT1 Antibody
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中文名稱：NIT1兔多克隆抗體
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貨號：CSB-PA768228LA01HU
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規格：￥440
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圖片：
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Western Blot
Positive WB detected in: Mouse brain tissue, Rat brain tissue
All lanes: NIT1 antibody at 3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 36, 32, 41, 35, 27 kDa
Observed band size: 36 kDa -
IHC image of CSB-PA768228LA01HU diluted at 1:1200 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. -
Immunofluorescence staining of HepG2 cells with CSB-PA768228LA01HU at 1:400, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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其他：
產品詳情
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產品名稱：Rabbit anti-Homo sapiens (Human) NIT1 Polyclonal antibody
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Uniprot No.：
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基因名：NIT1
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別名：MGC57670 antibody; NIT1 antibody; NIT1_HUMAN antibody; Nitrilase 1 antibody; Nitrilase homolog 1 antibody
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宿主：Rabbit
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反應種屬：Human, Mouse, Rat
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免疫原：Recombinant Human Deaminated glutathione amidase protein (1-243AA)
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免疫原種屬：Homo sapiens (Human)
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標記方式：Non-conjugated
本頁面中的產品，NIT1 Antibody (CSB-PA768228LA01HU)，的標記方式是Non-conjugated。對于NIT1 Antibody，我們還提供其他標記。見下表：
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克隆類型：Polyclonal
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抗體亞型：IgG
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純化方式：>95%, Protein G purified
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 -
產品提供形式：Liquid
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應用范圍：ELISA, WB, IHC, IF
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推薦稀釋比：
Application Recommended Dilution WB 1:500-1:5000 IHC 1:1000-1:2000 IF 1:200-1:500 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產品評價
相關產品
靶點詳情
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功能：Catalyzes the hydrolysis of the amide bond in N-(4-oxoglutarate)-L-cysteinylglycine (deaminated glutathione), a metabolite repair reaction to dispose of the harmful deaminated glutathione. Plays a role in cell growth and apoptosis: loss of expression promotes cell growth, resistance to DNA damage stress and increased incidence to NMBA-induced tumors. Has tumor suppressor properties that enhances the apoptotic responsiveness in cancer cells; this effect is additive to the tumor suppressor activity of FHIT. It is also a negative regulator of primary T-cells.
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基因功能參考文獻：
- Data show that cisplatin response was enhanced in human lung cancer cells when Nit1 was knocked down and mice Nit1-/-:KrasG12D/+ tumors showed increased sensitivity to cisplatin in vivo. PMID: 26967383
- Human NIT1/2 are not involved in the metabolism of vildagliptin. PMID: 25008847
- Nit1 and Fhit share tumor suppressor signaling pathways, while localization of the NIT1 gene at a stable chromosome site explains the paucity of gene alterations and in frequent loss of expression of the NIT1 gene in human malignancies. PMID: 16864578
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亞細胞定位：[Isoform 2]: Mitochondrion.; [Isoform 1]: Cytoplasm.
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蛋白家族：Carbon-nitrogen hydrolase superfamily, NIT1/NIT2 family
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組織特異性：Detected in heart, brain, placenta, liver, skeletal muscle, kidney and pancreas.
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數據庫鏈接：
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