HECW2 Antibody

中文名稱：

HECW2兔多克隆抗體
貨號：

CSB-PA889188LA01HU
規格：

￥440
圖片：
Western Blot
Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, 293T whole cell lysate
All lanes: HECW2 antibody at 3.5μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 176, 136 kDa
Observed band size: 176 kDa

IHC image of CSB-PA889188LA01HU diluted at 1:500 and staining in paraffin-embedded human glioma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-PA889188LA01HU diluted at 1:500 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of A549 cells with CSB-PA889188LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
其他：

產品詳情
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靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) HECW2 Polyclonal antibody
Uniprot No.：

Q9P2P5
基因名：

HECW2
別名：

C2 and WW domain-containing protein 2 antibody; E3 ubiquitin protein ligase HECW2 antibody; E3 ubiquitin-protein ligase HECW2 antibody; HECT antibody; HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 antibody; Hecw2 antibody; HECW2_HUMAN antibody; KIAA130 antibody; KIAA1301 antibody; NEDD4 like E3 ubiquitin protein ligase 2 antibody; NEDD4 Related E3 Ubiquitin Ligase antibody; NEDD4-like E3 ubiquitin-protein ligase 2 antibody
宿主：

Rabbit
反應種屬：

Human
免疫原：

Recombinant Human E3 ubiquitin-protein ligase HECW2 protein (495-641AA)
免疫原種屬：

Homo sapiens (Human)

標記方式：

Non-conjugated

本頁面中的產品，HECW2 Antibody (CSB-PA889188LA01HU)，的標記方式是Non-conjugated。對于HECW2 Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產品名稱	應用
HRP	CSB-PA889188LB01HU	HECW2 Antibody, HRP conjugated	ELISA
FITC	CSB-PA889188LC01HU	HECW2 Antibody, FITC conjugated
Biotin	CSB-PA889188LD01HU	HECW2 Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC, IF

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:500-1:1000
IF	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

E3 ubiquitin-protein ligase that mediates ubiquitination of TP73. Acts to stabilize TP73 and enhance activation of transcription by TP73. Involved in the regulation of mitotic metaphase/anaphase transition.
基因功能參考文獻：
1. Results show that HECW2 interacts with two lamin A-binding proteins: proliferating cell nuclear antigen (PCNA), via a canonical PCNA-interacting protein (PIP) motif, and lamin B1. HECW2 mediates their ubiquitination and targets them for proteasomal degradation. PMID: 29753763
2. HECW2, a novel EC ubiquitin E3 ligase, plays a critical role in stabilizing endothelial cell-to-cell junctions by regulating AMOT-like 1 (AMOTL1) stability. PMID: 27498087
3. HECW2 is an ubiquitin ligase that stabilises p73, a crucial mediator of neurodevelopment and neurogenesis. This study implicates pathogenic genetic variants in HECW2 as potential causes of neurodevelopmental disorders in humans. PMID: 27389779
4. This work lends further support to previously identified candidate gene HECW2 as a novel candidate gene in intellectual disability and epilepsy. In 39 patient-parent trios, 29 de novo mutations in coding sequence were identified. PMID: 27334371
5. Low HECW2 expression is associated with cervical cancer. PMID: 25156441
6. NEDL2 is a novel substrate of APC/C-Cdh1 as cells exit mitosis and functions as a regulator of the metaphase to anaphase transition PMID: 24163370
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相關疾病：

Neurodevelopmental disorder with hypotonia, seizures, and absent language (NDHSAL)
亞細胞定位：

Cytoplasm. Cytoplasm, cytoskeleton, spindle.
組織特異性：

Predominantly expressed in adult brain, lung and heart.
數據庫鏈接：

HGNC: 29853

OMIM: 617245

KEGG: hsa:57520

STRING: 9606.ENSP00000260983

UniGene: Hs.633212

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