HAT1 Antibody

中文名稱：

HAT1兔多克隆抗體
貨號：

CSB-PA010143EA01HU
規格：

￥440
圖片：
Western Blot
Positive WB detected in: MCF-7 whole cell lysate, Rat spleen tissue
All lanes: HAT1 antibody at 3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 50, 40 kDa
Observed band size: 50 kDa

Western Blot
Positive WB detected in: 293 whole cell lysate
All lanes: HAT1 antibody at 3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 50, 40 kDa
Observed band size: 50 kDa

Western Blot
WT: Wild-type 293 cells
KO: Knockout 293 cells

Immunofluorescent analysis of HepG2 cells using CSB-PA010143EA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) HAT1 Polyclonal antibody
Uniprot No.：

O14929
基因名：

HAT1
別名：

HAT 1 antibody; hat1 antibody; HAT1_HUMAN antibody; Histidine aminotransferase 1 antibody; Histone acetyltransferase 1 antibody; Histone acetyltransferase 1 antibody; Histone acetyltransferase type B catalytic subunit antibody; KAT1 antibody
宿主：

Rabbit
反應種屬：

Human, Rat
免疫原：

Recombinant Human Histone acetyltransferase type B catalytic subunit protein (245-419AA)
免疫原種屬：

Homo sapiens (Human)

標記方式：

Non-conjugated

本頁面中的產品，HAT1 Antibody (CSB-PA010143EA01HU)，的標記方式是Non-conjugated。對于HAT1 Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產品名稱	應用
HRP	CSB-PA010143EB01HU	HAT1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA010143EC01HU	HAT1 Antibody, FITC conjugated
Biotin	CSB-PA010143ED01HU	HAT1 Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IF

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
IF	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Histone acetyltransferase that plays a role in different biological processes including cell cycle progression, glucose metabolism, histone production or DNA damage repair. Coordinates histone production and acetylation via H4 promoter binding. Acetylates histone H4 at 'Lys-5' (H4K5ac) and 'Lys-12' (H4K12ac) and, to a lesser extent, histone H2A at 'Lys-5' (H2AK5ac). Drives H4 production by chromatin binding to support chromatin replication and acetylation. Since transcription of H4 genes is tightly coupled to S-phase, plays an important role in S-phase entry and progression. Promotes homologous recombination in DNA repair by facilitating histone turnover and incorporation of acetylated H3.3 at sites of double-strand breaks. In addition, acetylates other substrates such as chromatin-related proteins. Acetylates also RSAD2 which mediates the interaction of ubiquitin ligase UBE4A with RSAD2 leading to RSAD2 ubiquitination and subsequent degradation.; (Microbial infection) Contributes to hepatitis B virus (HBV) replication by acetylating histone H4 at the sites of 'Lys-5' and 'Lys-12' on the covalently closed circular DNA (cccDNA) minichromosome leading to its accumulation within the host cell.
基因功能參考文獻：
1. The expression of HAT1 and MYST1, did not differ between drug-sensitive and -resistant cells PMID: 18458078
2. Human histone acetyltransferase 1 (Hat1) acetylates lysine 5 of histone H2A in vivo. PMID: 24682716
3. The high-resolution crystal structure of a Hat1p/Hat2p/CoA/H4 peptide complex was solved and it was found that the H4 tail interacts with both Hat1p and Hat2p, by which substrate recruitment is facilitated. It was discovered that H3 N-terminal peptides can bind to the Hat2p WD40 domain. PMID: 24835250
4. Signalling from Toll-like or TNF-alpha receptors triggers the calcium/calmodulin-dependent protein kinase (CaMK2) to activate histone acetyltransferase-1 (HAT1), which then acetylates the transcriptional regulator PLZF. Acetylation of PLZF promotes the assembly of a repressor complex incorporating HDAC3 and the NF-kappa B p50 subunit that limits the NF-kappa B response. PMID: 25865065
5. HAT1 is involved in the STAT5 binding to the Bcl2L12 promoter. HAT1 increased the expression of Bcl2L12. Bcl2L12 mediated the effects of HAT1 on suppressing NPC cell apoptosis. Absorption of the HAT1 shRNA plasmid-carrying liposomes induced NPC cell apoptosis. In conclusion, inhibition of HAT1 can induce NPC cell apoptosis via increasing Bcl2L12 expression, which can be a potential therapy for NPC treatment. PMID: 29621521
6. HAT1 was upregulated in hepatocellular carcinoma.HAT1 acts as an oncogenic protein promoting cell proliferation and inducing cisplatin resistance in hepatocellular carcinoma. PMID: 27938492
7. Nuclear HDAC2 immunopositivity was significantly higher in actinic cheilitis (AC) when compared with lip squamous cell carcinoma (LSCC). HDAC1 and HAT1 nuclear immunostaining were higher in AC, with no statistical significance. When comparing data with our previous study, we found a positive correlation between HDAC1 X DNMT1/DNMT3b, HDAC2 X DNMT3b, and HAT1 X DNMT1/DNMT3b for certain studied groups. PMID: 28107582
8. AMPK phosphorylates DNMT1, RBBP7, and HAT1 and increases interactions of DNMT1, RBBP7, and HAT1. PMID: 28143904
9. Results show a novel interaction of the Lsm4 RGG domain with HAT1 and RBBP7, leading to the possibility of a posttranslational modifications network involved in mRNP regulation. PMID: 27247266
10. These data indicated that miR-486 aggravate the cholesterol accumulation in THP-1 cells by targeting HAT1. PMID: 26654953
11. The expression of HAT1 was higher in the primary Esophageal carcinoma tumors and adjacent tissue as compared to that of the normal esophageal tissue and HAT1 expression was directly correlated with the poor tumor differentiation PMID: 25120766
12. Data indicate that HAT1 is required for homologous recombination in DNA repair. PMID: 23653357
13. the role of Hat1 in DNA damage repair is evolutionarily conserved, the ability of H3 acetylation to compensate for Hat1 deletion appears to be more variable PMID: 17052979
14. HAT gene expression is required for cisplatin resistance and Clock and Tip60 regulate not only transcription, but also DNA repair, through periodic histone acetylation PMID: 18458078
顯示更多

收起更多
亞細胞定位：

[Isoform A]: Nucleus matrix. Mitochondrion.; [Isoform B]: Cytoplasm. Nucleus. Nucleus matrix. Nucleus, nucleoplasm.
蛋白家族：

HAT1 family
數據庫鏈接：

HGNC: 4821

OMIM: 603053

KEGG: hsa:8520

STRING: 9606.ENSP00000264108

UniGene: Hs.632532

Most popular with customers

YWHAG Recombinant Monoclonal Antibody

Applications: ELISA, WB, IHC

Species Reactivity: Human
YWHAB Recombinant Monoclonal Antibody

Applications: ELISA, WB, IHC, IF, FC

Species Reactivity: Human, Mouse, Rat
Phospho-YAP1 (S127) Recombinant Monoclonal Antibody

Applications: ELISA, WB, IHC

Species Reactivity: Human
XRCC5 Recombinant Monoclonal Antibody

Applications: ELISA, WB, IHC, IF

Species Reactivity: Human
XPNPEP1 Recombinant Monoclonal Antibody

Applications: ELISA, IHC, FC

Species Reactivity: Human
XDH Recombinant Monoclonal Antibody

Applications: ELISA, WB, IHC

Species Reactivity: Human
WT1 Recombinant Monoclonal Antibody

Applications: ELISA, WB, IHC

Species Reactivity: Human
WFDC2 Recombinant Monoclonal Antibody

Applications: ELISA, WB, FC

Species Reactivity: Human