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中文名稱：EIF3G兔多克隆抗體
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貨號：CSB-PA03099A0Rb
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規(guī)格：￥440
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圖片：
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IHC image of CSB-PA03099A0Rb diluted at 1:400 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. -
IHC image of CSB-PA03099A0Rb diluted at 1:400 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. -
Immunofluorescence staining of HepG2 cells with CSB-PA03099A0Rb at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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其他：
產(chǎn)品詳情
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產(chǎn)品名稱：Rabbit anti-Homo sapiens (Human) EIF3G Polyclonal antibody
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Uniprot No.：
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基因名：EIF3G
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別名：44kDa antibody; Anti EIF3S4 antibody; eIF 3 delta antibody; eIF 3 RNA binding subunit antibody; eIF-3 RNA-binding subunit antibody; eIF-3-delta antibody; eIF3 delta antibody; eIF3 p42 antibody; eIF3 p44 antibody; eIF3g antibody; EIF3G_HUMAN antibody; EIF3S4 antibody; EIF3S4 antibody antibody; EIF3S4; formerly antibody; Eukaryotic translation initiation factor 3 antibody; Eukaryotic translation initiation factor 3 RNA binding subunit antibody; Eukaryotic translation initiation factor 3 RNA-binding subunit antibody; Eukaryotic translation initiation factor 3 subunit 4 antibody; Eukaryotic translation initiation factor 3 subunit 4 delta 44 kD antibody; Eukaryotic translation initiation factor 3 subunit G antibody; Eukaryotic translation initiation factor 3 subunit p42 antibody; Eukaryotic translation initiation factor 3; subunit 4; formerly antibody; Subunit 4 (delta 44kD) antibody; Subunit 4 delta antibody
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宿主：Rabbit
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反應(yīng)種屬：Human
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免疫原：Recombinant Human Eukaryotic translation initiation factor 3 subunit G protein (2-320AA)
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免疫原種屬：Homo sapiens (Human)
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標(biāo)記方式：Non-conjugated
本頁面中的產(chǎn)品，EIF3G Antibody (CSB-PA03099A0Rb)，的標(biāo)記方式是Non-conjugated。對于EIF3G Antibody，我們還提供其他標(biāo)記。見下表：
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克隆類型：Polyclonal
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抗體亞型：IgG
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純化方式：>95%, Protein G purified
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 -
產(chǎn)品提供形式：Liquid
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應(yīng)用范圍：ELISA, IHC, IF
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推薦稀釋比：
Application Recommended Dilution IHC 1:200-1:500 IF 1:50-1:200 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
引用文獻
- System-wide Profiling of RNA-Binding Proteins Uncovers Key Regulators of Virus Infection Garcia-Moreno M, et al,Molecular Cell,2019
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點詳情
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功能：RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression. This subunit can bind 18S rRNA.; (Microbial infection) In case of FCV infection, plays a role in the ribosomal termination-reinitiation event leading to the translation of VP2.
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基因功能參考文獻：
- The disease-associated allele increases EIF3G mRNA expression. EIF3G is located in the narcolepsy risk locus and EIF3G expression correlates with PPAN and P2RY11 expression. PMID: 25669430
- Important roles for eIF3g in the translation initiation machinery and in DNA degradation during apoptosis. PMID: 24080033
- down-regulation of eIF3g inhibits the efficiency of nonsense-mediated mRNA decay, which is tightly coupled to CT but not to ET PMID: 22493286
- Furthermore, we found that overexpression of DISC1 in SH-SY5Y cells induces the assembly of eIF3- and TIA-1-positive stress granules (SGs), discrete cytoplasmic granules formed in response to environmental stresses. PMID: 16243297
- AIF overexpression specifically resulted in the activation of caspase-7, thereby amplifying the inhibition of protein PMID: 17094969
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亞細(xì)胞定位：Cytoplasm. Nucleus. Cytoplasm, perinuclear region. Note=Colocalizes with AIFM1 in the nucleus and perinuclear region.
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蛋白家族：EIF-3 subunit G family
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數(shù)據(jù)庫鏈接：
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