DTX3 Antibody

說(shuō)明書

中文名稱：

DTX3兔多克隆抗體
貨號(hào)：

CSB-PA839833LA01HU
規(guī)格：

￥440
圖片：
IHC image of CSB-PA839833LA01HU diluted at 1:100 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of SH-SY5Y cells with CSB-PA839833LA01HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
其他：

產(chǎn)品詳情

產(chǎn)品名稱：

Rabbit anti-Homo sapiens (Human) DTX3 Polyclonal antibody
Uniprot No.：

Q8N9I9
基因名：

DTX3
別名：

Deltex3 antibody; Dtx3 antibody; DTX3_HUMAN antibody; Protein deltex 3 antibody; Protein deltex-3 antibody; RING finger protein 154 antibody; RNF154 antibody
宿主：

Rabbit
反應(yīng)種屬：

Human
免疫原：

Recombinant Human Probable E3 ubiquitin-protein ligase DTX3 protein (90-347AA)
免疫原種屬：

Homo sapiens (Human)

標(biāo)記方式：

Non-conjugated

本頁(yè)面中的產(chǎn)品，DTX3 Antibody (CSB-PA839833LA01HU)，的標(biāo)記方式是Non-conjugated。對(duì)于DTX3 Antibody，我們還提供其他標(biāo)記。見下表：

標(biāo)記方式	貨號(hào)	產(chǎn)品名稱	應(yīng)用
HRP	CSB-PA839833LB01HU	DTX3 Antibody, HRP conjugated	ELISA
FITC	CSB-PA839833LC01HU	DTX3 Antibody, FITC conjugated
Biotin	CSB-PA839833LD01HU	DTX3 Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

Antigen Affinity Purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, IHC, IF

推薦稀釋比：

Application	Recommended Dilution
IHC	1:20-1:200
IF	1:50-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲(chǔ)存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

功能：

Regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations. Probably acts both as a positive and negative regulator of Notch, depending on the developmental and cell context. Functions as an ubiquitin ligase protein in vitro, suggesting that it may regulate the Notch pathway via some ubiquitin ligase activity.
基因功能參考文獻(xiàn)：
1. We show here that both molecules (Deltex (DTX) and AIP4) interact and partially colocalize to endocytic vesicles, and that AIP4 targets DTX for lysosomal degradation. PMID: 17028573
亞細(xì)胞定位：

Cytoplasm.
蛋白家族：

Deltex family
數(shù)據(jù)庫(kù)鏈接：

HGNC: 24457

OMIM: 613142

KEGG: hsa:196403

STRING: 9606.ENSP00000338050

UniGene: Hs.32374

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