DSG3 Antibody

中文名稱：

DSG3兔多克隆抗體
貨號：

CSB-PA007205LA01HU
規格：

￥440
圖片：
IHC image of CSB-PA007205LA01HU diluted at 1:500 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of Hela cells with CSB-PA007205LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
其他：

產品詳情
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靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) DSG3 Polyclonal antibody
Uniprot No.：

P32926
基因名：

DSG3
別名：

130 kD pemphigus vulgaris antigen antibody; 130 kDa pemphigus vulgaris antigen antibody; Balding antibody; Cadherin family member 6 antibody; CDHF 6 antibody; CDHF6 antibody; Desmoglein 3 antibody; Desmoglein 3 precursor antibody; Desmoglein-3 antibody; Desmoglein3 antibody; DKFZp686P23184 antibody; DSG 3 antibody; DSG3 antibody; DSG3_HUMAN antibody; Pemphigus vulgaris antigen antibody; PVA antibody
宿主：

Rabbit
反應種屬：

Human
免疫原：

Recombinant Human Desmoglein-3 protein (70-615AA)
免疫原種屬：

Homo sapiens (Human)

標記方式：

Non-conjugated

本頁面中的產品，DSG3 Antibody (CSB-PA007205LA01HU)，的標記方式是Non-conjugated。對于DSG3 Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產品名稱	應用
HRP	CSB-PA007205LB01HU	DSG3 Antibody, HRP conjugated	ELISA
FITC	CSB-PA007205LC01HU	DSG3 Antibody, FITC conjugated
Biotin	CSB-PA007205LD01HU	DSG3 Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, IHC, IF

推薦稀釋比：

Application	Recommended Dilution
IHC	1:500-1:1000
IF	1:50-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.
基因功能參考文獻：
1. flotillin has a role in desmosomal adhesion and Pemphigus vulgaris-like localisation of desmoglein-3 in human keratinocytes PMID: 27346727
2. our findings suggest that Dsg3 functions as a key in cell-cell adhesion through at least a mechanism of regulating E-cadherin membrane trafficking PMID: 27254775
3. Desmoglein 3 Order and Dynamics in Desmosomes PMID: 29212005
4. Data indicate that all 3 monoclonal antibodies (mAbs) targeted the same extracellular cadherin (EC) domain on desmoglein 3 (Dsg3), caused Dsg3 internalization in primary keratinocytes, and caused suprabasal blisters in skin. PMID: 27304671
5. DSG3 was a key prognostic factor and predictor for neoadjuvant concurrent chemoradiotherapy response in rectal cancer patients. PMID: 27040321
6. Treg cells exert a clear down-regulatory effect on the Dsg3-driven T-cell response and, accordingly, the formation of Dsg3-specific IgG antibodies PMID: 26661498
7. Data identify DSG3 as a negative prognostic biomarker in resected pancreatic ductal adenocarcinoma, as high DSG3 expression is associated with poor overall survival and poor tumour-specific survival. PMID: 26469831
8. this study directly demonstrates the occurrence of desmoglein 3 molecules outside of desmosomes and reveales that the adhesive properties of these molecules do differ depending on their localization. PMID: 25510735
9. DSG2 and DSG3 might be potential diagnostic markers for squamous cell carcinoma of the lung and DSG3 could be a potential differentiation marker for lung cancer. PMID: 25468811
10. alterations of their expression suggest a role of Dsg3 and gamma-catenin (additionally to E-cadherin/beta-catenin) as biomarkers of malignant transformation risk of oral dysplasia and the biological behavior (aggressiveness) of oral cancer, respectively PMID: 23430339
11. the Dsg3 epitopes targeted by pathogenic mPV IgG are human specific in mice PMID: 25695683
12. Pemphigus vulgaris is characterized by low IgG reactivities to specific self-antigens along with high IgG reactivity to desmoglein 3. PMID: 24820664
13. High DSG3 mRNA expression is associated with esophageal squamous cell carcinoma. PMID: 24568510
14. Pemphigus vulgaris autoimmune globulin induces Src-dependent tyrosine-phosphorylation of PKP3 and its detachment from DSG3. PMID: 24328683
15. Dsg3 did not associate with rafts in cells lacking desmosomal proteins. PMID: 24498201
16. this study identifies a novel Dsg3-mediated c-Jun/AP-1 regulatory mechanism and PKC-dependent Ezrin phosphorylation that could be responsible for Dsg3-associated cancer metastasis. PMID: 23752190
17. DSG3 functions as an oncogene and facilitates cancer growth and invasion in head and neck cancer cells PMID: 23737966
18. The conformational Dsg3 ELISA index reflected the pathogenicity of anti-Dsg3 antibodies more accurately than the conventional Dsg3 ELISA index in pemphigus vulgaris. PMID: 23602628
19. MAC387 and desmoglein-3 are reliable diagnostic markers for supporting the morphologic impression of squamous differentiation in urinary bladder carcinoma. PMID: 23806524
20. All 3 markers were studied independently and were associated with tumor percentage in metastatic lymph nodes. PVA had the strongest correlation, followed by PTHrP and then TACSTD1. PMID: 23625795
21. provide a proof of principle supporting that ultrasensitive nanostructured assay systems for DSG3 can be exploited to detect micrometastatic HNSCC lesions in lymph nodes, which can improve the diagnosis PMID: 23010602
22. The cis-adhesive interface on extracellular subdomain EC1 recognized by the pathogenic antibody PVA224 is the primary target of the autoantibodies present in the serum of pemiphigus vulgaris patients. PMID: 22996451
23. an important novel function for Dsg3 in promoting actin dynamics through regulating Rac1 and Cdc42 activation in epithelial cells. PMID: 22796473
24. a useful immunohistochemical marker for differentiation of lung squamous cell carcinoma and adenocarcinoma from other subtypes PMID: 22495379
25. In paraneoplastic pempigus and pemphigus vulgaris, IgG autoantibodies to DSG3 may be found in autoantibody profiling. PMID: 22318391
26. Report mapping of B cell epitopes on desmoglein 3 in pemphigus vulgaris patients by the use of overlapping peptides. PMID: 22261006
27. These findings suggest a novel function for Dsg3 in the control of E-cadherin-Src signalling and cell-cell adhesion. PMID: 22294297
28. These data indicate a contribution of Dsg depletion to pemphigus vulgaris pathogenesis dependent on Ca(2+)-induced differentiation. PMID: 21864491
29. Silencing desmoglein 3 caused defects in cell-cell adhesion and concomitant reduction in cell proliferation in both HaCaT and MDCK cells. PMID: 21702856
30. Dsg3, as an up-stream regulator of Src activity, helps regulate adherens junction formation through its interaction with E-cadherin PMID: 21151980
31. The genetic background of the local population may explain why pemphigus occurs more commonly than bullous pemphigoid in Northwestern Romania compared with the population of Western Europe PMID: 20618495
32. results argue against the hypothesis that DSG3 coding variants play a role in pemphigus vulgaris susceptibility PMID: 19678820
33. Data strongly support the view that desmoglein 3 contributes to the regulation of epidermal differentiation. PMID: 12138195
34. The appearance of DSG3-reactive Th2 cells is constant at different stages of pemphigus vulgaris (PV), while DSG3-reactive Th1 cells are detected at a significantly higher frequency in chronic active PV. PMID: 12496453
35. In trichilemmal keratinization in follicle, and in cysts in these areas, desmoglein 3 expressed throughout outer root sheath and cyst wall. In areas of epidermal-like keratinization, desmoglein 3 expression was limited mainly to the basal layer. PMID: 12787134
36. a novel negative marker for epidermal stem cell-containing population of keratinocytes PMID: 12953062
37. Dsg3(AA145-192)-specific cells preferentially utilize the TCRVbeta13 gene, while Dsg3(AA240-303)- and Dsg3 (AA570-614)-specific cells utilize Vbeta7 and Vbeta17 genes, respectively PMID: 14675184
38. The five genes, and three of the encoded proteins, were shown differentially expressed between a group of keratoconus patients and a reference group using different techniques PMID: 16015083
39. Dsg3 endocytosis, keratin filament retraction, and the loss of keratinocyte cell-cell adhesion are coordinated responses to PV IgG PMID: 16377623
40. autoantibodies against desmoglein 3 (Dsg3) have been detected in sera from patients with fogo selvagem PMID: 16763546
41. analysis of desmoglein 3 ectodomain in pemphigus vulgaris PMID: 16842599
42. Overexpression of DSG3 is associated with head and neck cancer PMID: 16878157
43. Desmoglein 3 status indicated a poor prognosis in lung cancers. PMID: 17084439
44. The T cellular autoimmune response against immunodominant peptides of Dsg3 in patients with pemphigus vulgaris is monitored with highly specific HLA-DRbeta1*0402 tetramers. PMID: 17113829
45. These data indicate that Dsg3(dim) populations from primary human adult keratinocytes and long-term established keratinocyte lines possess certain stem/progenitor cell-like properties. PMID: 17255524
46. Data suggest that although the desmoglein (Dsg)3 depletion is not indicative for adhesive strength, it may indicate pathogenic changes within the cell and it plays a role in skin fragility or susceptibility to blister formation in pemphigus patients. PMID: 17428808
47. Reduction of Dsg3 might be relevant to blister formation in pemphigus vulgaris. PMID: 17431647
48. pemphigus vulgaris-IgG-dependent uPA activation is not related to anti-Dsg3 antibody activity PMID: 17532189
49. Autoantibody from pemphigus vulgaris sera react with non-conformational epitopes of desmoglein 3. PMID: 18095943
50. results indicate that the membrane proximal region in the IA region of Dsg3 is necessary for complex formation with P120-catenin and to maintain free Dsg3 at the cell surface before it is integrated into desmosomes PMID: 18343367
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亞細胞定位：

Cell membrane; Single-pass type I membrane protein. Cell junction, desmosome.
組織特異性：

Epidermis, tongue, tonsil, esophagus and carcinomas.
數據庫鏈接：

HGNC: 3050

OMIM: 169615

KEGG: hsa:1830

STRING: 9606.ENSP00000257189

UniGene: Hs.1925

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