DMC1 Antibody

中文名稱：

DMC1兔多克隆抗體
貨號：

CSB-PA617920LA01HU
規格：

￥440
圖片：
Western Blot
Positive WB detected in: Raji whole cell lysate, K562 whole cell lysate
All lanes: DMC1 antibody at 3.2µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 38, 32 kDa
Observed band size: 38 kDa

Immunohistochemistry analysis of human testis tissue using CSB-PA617920LA01HU at dilution of 1:100

Immunofluorescent analysis of Hela cells using CSB-PA617920LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) DMC1 Polyclonal antibody
Uniprot No.：

Q14565
基因名：

DMC1
別名：

Disrupted meiotic cDNA 1 homolog antibody; Disrupted meiotic cDNA 1; yeast; homolog of antibody; dJ199H16.1 antibody; DMC 1 antibody; DMC1 antibody; DMC1 dosage suppressor of mck1 homolog antibody; DMC1 dosage suppressor of mck1 homolog meiosis specific homologous recombination (yeast) antibody; DMC1 homologue antibody; DMC1_HUMAN antibody; DMC1H antibody; DNA meiotic recombinase 1 antibody; HsLim15 antibody; LIM15 antibody; Meiotic recombination protein DMC1/LIM15 homolog antibody; MGC150472 antibody; MGC150473 antibody
宿主：

Rabbit
反應種屬：

Human
免疫原：

Recombinant Human Meiotic recombination protein DMC1/LIM15 homolog protein (5-153AA)
免疫原種屬：

Homo sapiens (Human)

標記方式：

Non-conjugated

本頁面中的產品，DMC1 Antibody (CSB-PA617920LA01HU)，的標記方式是Non-conjugated。對于DMC1 Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產品名稱	應用
HRP	CSB-PA617920LB01HU	DMC1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA617920LC01HU	DMC1 Antibody, FITC conjugated
Biotin	CSB-PA617920LD01HU	DMC1 Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC, IF

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:20-1:200
IF	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Participates in meiotic recombination, specifically in homologous strand assimilation, which is required for the resolution of meiotic double-strand breaks.
基因功能參考文獻：
1. Data indicate that ahomologous pairing by DMC1 protein or RAD51 recombinase in chromatin containing nucleosome-depleted double-stranded DNA (dsDNA) regions. PMID: 27052786
2. BRCA2 protein stimulates DMC1-mediated DNA strand exchange between RPA-ssDNA complexes and duplex DNA, thus identifying BRCA2 as a mediator of DMC1 recombination function. PMID: 26976601
3. Dmc1 can reject divergent DNA sequences while bypassing a few mismatches in the DNA sequence. PMID: 26709229
4. In contrast to RAD51, stabilization of the presynaptic filament via ATP hydrolysis attenuation is insufficient for enhancement of the DMC1-catalyzed recombination reaction. PMID: 26088134
5. truncated DMC1 octamers further stack with alternate polarity into a filament PMID: 23545642
6. results suggested mutations in the coding sequence of DMC1 are not associated with premature ovarian failure in Chinese women PMID: 23265958
7. conserved lysine in the Walker A motif of hDMC1 is critical for ATP binding. PMID: 23182424
8. The results suggested that PSF may function as an activator for the meiosis-specific recombinase DMC1. PMID: 22156371
9. D-loops in a human DMC1-driven reaction are substantially more resistant to dissociation by branch-migration proteins such as RAD54 than those formed by RAD51. PMID: 21151113
10. the Dmc1 filament formed on single-stranded DNA has a mass per unit length expected from approximately 6.5 subunits per turn PMID: 20600108
11. p53 might be involved in homologous recombination and/or checkpoint function by directly binding to DMC1 protein to repress genomic instability in meiotic germ cells PMID: 14764457
12. The monomeric structure of the Dmc1 protein closely resembled those of the human and archaeal Rad51 proteins. We found another hydrogen bonding interaction at the polymer interface. PMID: 15125839
13. hDmc1-mediated DNA recombination initiates through the nucleation of hDmc1 onto ssDNA to form a helical nucleoprotein filament PMID: 15164066
14. N-terminal domain of DMC1 is required for the formation of the octamer, which may support the proper DNA binding activity of the DMC1 protein. PMID: 15917243
15. activation of hDmc1 is mediated through conformational changes induced by free Ca2+ ion binding to a protein site that is distinct from the Mg2+.ATP-binding center PMID: 15917244
16. BRCA2 binds the meiosis-specific recombinase DMC1 and define the primary DMC1 interaction site to a 26 amino-acid region PMID: 17541404
17. Hop2/Mnd1 greatly stimulates Dmc1 to promote synaptic complex formation on long duplex DNAs PMID: 17639081
18. Data show that DMC1 mutations may be one explanation for premature ovarian failure. PMID: 18166824
19. The interaction of DMC1 and the homologue of the large subunit of CAF-1 is reported. PMID: 18355319
20. Results indicate that Rad51 and Dmc1 filaments are essentially identical with respect to several structural parameters, including persistence length, helical pitch, filament diameter, DNA base pairs per helical turn and helical handedness. PMID: 18535008
21. Biochemical analyses revealed that the human DMC1-M200V variant had reduced stability, and was moderately defective in catalyzing in vitro recombination reactions. PMID: 18566005
22. Reversibility, equilibration, and fidelity of strand exchange reaction between short oligonucleotides promoted by RecA protein from escherichia coli and human Rad51 and Dmc1 proteins. PMID: 19004837
23. DMC1-I37N polymorphism may be a source of improper meiotic recombination, causing meiotic defects in humans PMID: 19076215
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亞細胞定位：

Nucleus. Chromosome.
蛋白家族：

RecA family, DMC1 subfamily
數據庫鏈接：

HGNC: 2927

OMIM: 602721

KEGG: hsa:11144

STRING: 9606.ENSP00000216024

UniGene: Hs.339396

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