DMAP1 Antibody

中文名稱：

DMAP1兔多克隆抗體
貨號：

CSB-PA873611LA01HU
規格：

￥440
圖片：
Western Blot
Positive WB detected in: MCF-7 whole cell lysate, Colo320 whole cell lysate
All lanes: DMAP1 antibody at 11µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 53 kDa
Observed band size: 53 kDa

IHC image of CSB-PA873611LA01HU diluted at 1:400 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of Hela cells with CSB-PA873611LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
其他：

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靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) DMAP1 Polyclonal antibody
Uniprot No.：

Q9NPF5
基因名：

DMAP1
別名：

DMAP1 antibody; KIAA1425DNA methyltransferase 1-associated protein 1 antibody; DNMAP1 antibody; DNMT1-associated protein 1 antibody
宿主：

Rabbit
反應種屬：

Human
免疫原：

Recombinant Human DNA methyltransferase 1-associated protein 1 protein (250-300AA)
免疫原種屬：

Homo sapiens (Human)

標記方式：

Non-conjugated

本頁面中的產品，DMAP1 Antibody (CSB-PA873611LA01HU)，的標記方式是Non-conjugated。對于DMAP1 Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產品名稱	應用
HRP	CSB-PA873611LB01HU	DMAP1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA873611LC01HU	DMAP1 Antibody, FITC conjugated
Biotin	CSB-PA873611LD01HU	DMAP1 Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC, IF

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:200-1:500
IF	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Involved in transcription repression and activation. Its interaction with HDAC2 may provide a mechanism for histone deacetylation in heterochromatin following replication of DNA at late firing origins. Can also repress transcription independently of histone deacetylase activity. May specifically potentiate DAXX-mediated repression of glucocorticoid receptor-dependent transcription. Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Participates in the nuclear localization of URI1 and increases its transcriptional corepressor activity.
基因功能參考文獻：
1. A novel tumour suppressor gene, MDGA2, which is frequently inactivated by promoter methylation in Gastric cancer, exerts a tumour suppressive function by cooperating with DMAP1 to activate the p53/p21 signalling cascade. PMID: 26206665
2. Targeted knockdown of human DNA methyltransferase 1 expression restores the expression levels of tumor suppressor genes, thus inhibiting the proliferation of cholangiocarcinoma cell line cells. PMID: 24361215
3. These results suggest that DMAP1 is a critical regulator of ATM activity and function PMID: 23318425
4. positive staining for DNMT1 in clinical cancer specimens was significantly linked to lower rates of response to treatments and shorter survival of patients with pharyngeal cancer. PMID: 21284050
5. Knocking down DMAP1 caused hypomethylation of the DNA repair products. PMID: 20864525
6. MMTR is an intrinsic negative cell cycle regulator that modulates the CAK kinase activity via interaction with MAT1. PMID: 20920467
7. RGS6 inhibited the transcriptional repressor activity of DMAP1. PMID: 14734556
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亞細胞定位：

Nucleus. Cytoplasm. Note=Targeted to replication foci throughout S phase by DNMT1.
數據庫鏈接：

HGNC: 18291

OMIM: 605077

KEGG: hsa:55929

STRING: 9606.ENSP00000312697

UniGene: Hs.8008

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