CYP7B1 Antibody

中文名稱(chēng)：

CYP7B1兔多克隆抗體
貨號(hào)：

CSB-PA080001
規(guī)格：

￥1090
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

Uniprot No.：

O75881
基因名：

CYP7B1
別名：

25 hydroxycholesterol 7 alpha hydroxylase antibody; 25-hydroxycholesterol 7-alpha-hydroxylase antibody; CP7B antibody; CP7B1_HUMAN antibody; Cyp7b1 antibody; Cytochrome P450 7B1 antibody; Cytochrome P450 family 7 subfamily B polypeptide 1 antibody; Cytochrome P450 subfamily VIIB polypeptide 1 antibody; Oxysterol 7-alpha-hydroxylase antibody; Oxysterol 7alpha hydroxylase antibody
宿主：

Rabbit
反應(yīng)種屬：

Human
免疫原：

Synthesized peptide derived from the Internal region of Human CYP7B1.
免疫原種屬：

Homo sapiens (Human)
標(biāo)記方式：

Non-conjugated
抗體亞型：

IgG
純化方式：

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

WB, IHC, ELISA

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:100-1:300
ELISA	1:40000

Protocols：

Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
ELISA Protocol
儲(chǔ)存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

A cytochrome P450 monooxygenase involved in the metabolism of endogenous oxysterols and steroid hormones, including neurosteroids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase). Catalyzes the hydroxylation of carbon hydrogen bonds of steroids with a preference for 7-alpha position. Usually metabolizes steroids carrying a hydroxy group at position 3, functioning as a 3-hydroxy steroid 7-alpha hydroxylase. Hydroxylates oxysterols, including 25-hydroxycholesterol and (25R)-cholest-5-ene-3beta,26-diol toward 7-alpha hydroxy derivatives, which may be transported to the liver and converted to bile acids. Via its product 7-alpha,25-dihydroxycholesterol, a ligand for the chemotactic G protein-coupled receptor GPR183/EBI2, regulates B cell migration in germinal centers of lymphoid organs, thus guiding efficient maturation of plasma B cells and overall antigen-specific humoral immune response. 7-alpha hydroxylates neurosteroids, including 3beta-hydroxyandrost-5-en-17-one (dehydroepiandrosterone) and pregnenolone, both involved in hippocampus-associated memory and learning. Metabolizes androstanoids toward 6- or 7-alpha hydroxy derivatives.
基因功能參考文獻(xiàn)：
1. SPG11 and CYP7B1 were the most common cause of autosomal recessive hereditary spastic paraplegia in Greece. PMID: 26374131
2. miR17 induces epithelial-mesenchymal transition consistent with the cancer stem cell phenotype by regulating CYP7B1 expression in colon cancer. PMID: 27278684
3. The two novel variants cosegregated with pyramidal signs and autoimmune diseases suggesting that they might be susceptibility factors. PMID: 26370385
4. Data indicate two novel homozygous mutations (one frameshift and one missense mutation) detected in CYP7B1 (SPG5A), while no disease-causing mutation was identified for PNPLA6 (SPG39) and C19orf12 (SPG43). PMID: 26714052
5. Data indicated that the two GWAS-defined variants in the CYP7B1 region do not strongly influence HIV-1 infection susceptibility. PMID: 26399852
6. Using an agnostic omics approach to focus on the association of CWP with body mass index, we have confirmed a steroid hormone association and identified a genetic variant upstream of the CYP genes, which likely controls this response. PMID: 25915148
7. Spastic paraplegia type 5 has a higher frequency in Taiwanese than in other ethnic groups, associated with a CYP7B1 founder mutation and its phenotype is characterized by pronounced dorsal column sensory loss, with cerebellar ataxia in some patients. PMID: 24641183
8. The patient was homozygous for a mutation (c.1249C>T) in CYP7B1 that alters a highly conserved residue in oxysterol 7 alpha-hydroxylase previously reported in a family with hereditary spastic paraplegia type 5 PMID: 24658845
9. enduring sensory ataxia can be a pivotal sign in SPG5, and expands the phenotypic spectrum associated with mutations in CYP7B1 PMID: 24519355
10. 4 novel mutations described in hereditary spastic paraplegia type 5A: 1 frameshift (c.509 delT p.L170fs), 1 premature stop codon (c.334 C>T p.R112X), 1 amino acid changing (c.440 G>A p.G147D) and 1 duplication (c.945_947 dupGGC p.A316AA) PMID: 24117163
11. 21-hydroxy-pregnenolone was identified as a new substrate, and overall low activity toward pregnanes could be related to the increased potency of 7-hydroxy derivatives produced by CYP7B1. PMID: 24491228
12. investigation of CYP7B1-substrate binding modes PMID: 23180418
13. Description of a homology model for human CYP7B1 that provides valuable information on the active site architecture, along with docking studies that analyzed ligand-binding interactions. PMID: 21541746
14. Five CYP7B1 mutations, three of which are novel, were identified in four patients with hereditary spastic paraplegia type 5. PMID: 21214876
15. analysis of the first Japanese patient with an oxysterol 7alpha-hydroxylase deficiency associated with compound heterozygous mutations of the CYP7B1 gene [case report] PMID: 21567895
16. We identifies a Chinese family with hereditary spastic paraplegia due to compound heterozygous mutations in the CYP7B1 gene. PMID: 21452256
17. In Alzheimer's disease (AD). CYP7B mRNA was significantly decreased (approximately 50% decline; P<0.05) in dentate neurons from AD subjects compared with controls. PMID: 14521990
18. Promotor activity of the human oxysterol 7alpha-hydroxylase gene is suppressed by sterol response element binding protein. PMID: 15003524
19. Single polymorphism in the CYP7B1 gene is associated with phenotypic differences in an expression system and a widely different allele frequency in two ethnic populations, with great differences in the incidence of prostate cancer. PMID: 15007371
20. CYP7B catalyzes oxysterol 7alpha-hydroxylation within the human prostate epithelium and an ERbeta-specific agonist, 7HD, is produced. PMID: 15181079
21. the 7-hydroxylation catalysed by P4507B1 preferentially takes place on DHEA, 5alpha-androstane-3beta,17beta-diol and epiandrosterone with major and minor formation of 7alpha- and 7beta-hydroxylated derivatives, respectively [cyp7b1] PMID: 15698543
22. Increased CYP7B activity leads to higher levels of 7alpha-OH-DHEA in synovial fluid which may contribute to the maintenance of chronic inflammation observed in rheumatoid arthritis patients. PMID: 15751070
23. In particular, the data suggest that androgens may control intraprostatic levels of estrogen via regulation of CYP7B1-mediated metabolism. PMID: 16630558
24. Presence of both CYP7B1 and 11beta-HSD1 in human skin. PMID: 17467270
25. Results suggest that -204A/C polymorphism in the CYP7A1 gene does not relate with hypertriglyceridemia but may has an effect on serum triglyceride and apoCIII levels in patients with endogenous HTG. PMID: 17680536
26. Identification of CYP7B1 as a novel ROTalpha (NR1F1) target gene and a functional cross-talk between RORalpha and liver X receptor (NR1H3). PMID: 18055760
27. Sequence alterations within CYP7B1 implicate defective cholesterol homeostasis in motor-neuron degeneration. PMID: 18252231
28. tissue-specific steroid concentrations may have a strong impact on CYP7B1-dependent catalysis and thus on the levels of different CYP7B1-related steroids that can influence estrogen receptor beta signaling PMID: 18331353
29. regulation of CYP7B1 by ER can be mediated via the PI3K/Akt signal pathway, a regulatory pathway important for cellular survival and growth, suggest an important role for CYP7B1 in cellular growth, particularly in connection with estrogenic signalling. PMID: 18790053
30. Findings suggest CYP7B1 alterations to represent a rather frequent cause of hereditary spastic paraplegia that should be considered in patients with various clinical presentations. PMID: 18855023
31. screening of SPG5/CYP7B1 seems to have a low diagnostic yield in autosomal recessive and sporadic cases of spastic paraplegia, even in those with complicated clinical features. PMID: 19187859
32. we report the first Italian families with SPG5hereditary spastic paraplegia molecular characterization and describe two novel truncating mutations in CYP7B1. PMID: 19363635
33. Results confirm that CYP7B1 is the gene responsible for Spastic Paraplegia type 5. PMID: 19439420
34. CYP7B1 has multiple physiological functions and a role in liver failure in children and in neuropathy [review] PMID: 19687010
35. CYP7B1-mediated catalysis may play a role for control of the cellular levels of androgens, not only of estrogens. PMID: 19732851
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相關(guān)疾病：

Spastic paraplegia 5A, autosomal recessive (SPG5A); Congenital bile acid synthesis defect 3 (CBAS3)
亞細(xì)胞定位：

Endoplasmic reticulum membrane; Multi-pass membrane protein. Microsome membrane; Multi-pass membrane protein.
蛋白家族：

Cytochrome P450 family
組織特異性：

Widely expressed. Expressed in brain, testis, ovary, prostate, liver, colon, kidney, small intestine, thymus and spleen.
數(shù)據(jù)庫(kù)鏈接：

HGNC: 2652

OMIM: 270800

KEGG: hsa:9420

STRING: 9606.ENSP00000310721

UniGene: Hs.657330

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