Western blot All lanes: COL10A1 antibody at 4μg/ml + K562 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 66 kDa Observed band size: 66 kDa
Immunohistochemistry of paraffin-embedded human prostate tissue using CSB-PA005715ESR2HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA005715ESR2HU at dilution of 1:100
To synthesize the COL10A1 antibody, a partial recombinant protein of the human collagen alpha-1(X) chain protein corresponding to the 521-680 amino acids was used to immunize a rabbit. The resulting COL10A1 antibody is an unconjugated IgG isoform that underwent antigen affinity purification.
Rigorous quality control of this COL10A1 polyclonal antibody has been performed in multiple applications, including ELISA, WB, and IHC, to ensure its reliability. It has been demonstrated to be reactive with human COL10A1 protein. The COL10A1 protein plays a role in bone development, angiogenesis, cell migration, and cancer progression.
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貨期:
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用途:
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Type X collagen is a product of hypertrophic chondrocytes and has been localized to presumptive mineralization zones of hyaline cartilage.
基因功能參考文獻:
Plasma samples from lung cancer patients and healthy heavy-smokers controls were tested for levels of COL11A1 and COL10A1 (n = 57 each) and SPARC (n = 90 each). Higher plasma levels of COL10A1 were detected in patients (p = 0.001), a difference that was driven specifically by females (p < 0.001). No difference in COL11A1 levels between patients and controls was found PMID: 30227835
All affected individuals are heterozygous for the missense mutation collagen type X alpha 1 chain (COL10A1) rs111033552. PMID: 29234170
increased expression of stromal colXalpha1 and low TILs correlate with poor pathologic response in ER+/HER2+ breast tumors. Further studies are needed to confirm their predictive value and impact on long-term outcomes, and to determine whether this collagen exerts a protective effect on the cancer cells or simply reflects other factors within the tumor microenvironment PMID: 27090210
a novel sequence variation involving an unusual mutational site of the COL10A1 gene can cause mild metaphyseal chondrodysplasia PMID: 25542771
COL10A1 mutation 2005delC in a Chinese pedigree with Schmid type metaphyseal chondrodysplasia is close to the C-terminus of the protein sequence and may result in genetic heterogeneity of the Chinese population PMID: 25974987
Concentration of serum collagen type X levels correlated with cartilage degradation in osteoarthritis patients. PMID: 25245039
The results show that COL10A1 is a tumor biomarker upregulated in a wide variety of tumors including those of the breast, colon, bladder, stomach, esophagus, lung, testis, ovary and pancreas. PMID: 22894674
Yiqi Huayu Bushen Recipe increased the expression of aggrecan, decreased the expression of type X collagen, and promoted cell proliferation in cells from degenerated human intervertebral discs. PMID: 22015197
Genetic variation near FRK/COL10A1 and VEGFA are associated with advanced age-related macular degeneration. PMID: 21665990
MCDS is a rare genetic skeletal disorder caused by a collagen type X defect. Though much is known about the molecular pathology of the causative COL10A1 mutations, causal therapy of the disease is not yet available PMID: 21360259
a frameshift mutation leading to elongation of the deduced alpha1(X) chain associated with Metaphyseal Chondrodysplasia type Schmid PMID: 21447328
These results indicate that nitrogen-rich plasma polymerized surfaces inhibit COL10A1 expression via the suppression of COX-1. PMID: 20225218
speculate that complete loss of mutant transcripts yields COL10A1 haploinsufficiency and late clinical presentation while incomplete loss of mutant transcripts yields dominant-negative effects with early clinical presentation PMID: 20872587
The total expression of type X collagen in the concave side growth plates of the lower end vertebrae was higher than that in the same side growth plates of apex. PMID: 20073986
methylation-based COL10A1 gene silencing is established in cartilage tissue and human articular chondrocytes during chondrogenesis. PMID: 18759285
chains harboring Schmid metaphyseal chondrodysplasia NC1 domain mutations are selectively retained and degraded in stably transfected cells PMID: 11805116
The crystal structure at 2.0 A resolution of the human collagen X NC1 domain reveals an intimate trimeric assembly strengthened by a buried cluster of calcium ions. PMID: 11839302
The 4.6 kb promoter is able to drive specific expression of Col10a1 in hypertrophic cartilage. PMID: 15464363
exposure of the NC1 thiol may trigger the recognition and degradation of mutant collagen X chains PMID: 15695517
The effect of COL10A1 nonsense mutations in cartilage tissue has been examined in two patients, demonstrating that the mutant mRNA is completely removed by nonsense mediated mRNA decay PMID: 15880705
retinoids stimulate collagen X transcription IN chondrocytes PMID: 16598786
the triple-helical region of collagen X contains a specific DDR2 binding site that is capable of receptor activation PMID: 16806867
Type X collagen was not detected in any of atehrosclerotic plaques investigated in crural arteries. PMID: 17335825
Investigated a family affected with the Spahr type of metaphyseal chondrodysplasia. Sequencing of RMRP, and a haplotype analysis using highly informative markers around the COL10A1 excluded both genes from being pathogenic in this family. PMID: 18553549
HY(hypertrophy) box is the core element responsive to RUNX-2 in human COL10A1 promoter PMID: 19116917