CKAP5 Antibody

中文名稱：

CKAP5兔多克隆抗體
貨號：

CSB-PA617899LA01HU
規格：

￥440
圖片：
Western Blot
Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, Rat brain tissue
All lanes: CKAP5 antibody at 6µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 226, 219, 227 kDa
Observed band size: 226 kDa

IHC image of CSB-PA617899LA01HU diluted at 1:400 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-PA617899LA01HU diluted at 1:400 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of Hela cells with CSB-PA617899LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
其他：

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靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) CKAP5 Polyclonal antibody
Uniprot No.：

Q14008
基因名：

CKAP5
別名：

CKAP5 antibody; KIAA0097Cytoskeleton-associated protein 5 antibody; Colonic and hepatic tumor overexpressed gene protein antibody; Ch-TOG antibody
宿主：

Rabbit
反應種屬：

Human, Rat
免疫原：

Recombinant Human Cytoskeleton-associated protein 5 protein (1802-2019AA)
免疫原種屬：

Homo sapiens (Human)

標記方式：

Non-conjugated

本頁面中的產品，CKAP5 Antibody (CSB-PA617899LA01HU)，的標記方式是Non-conjugated。對于CKAP5 Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產品名稱	應用
HRP	CSB-PA617899LB01HU	CKAP5 Antibody, HRP conjugated	ELISA
FITC	CSB-PA617899LC01HU	CKAP5 Antibody, FITC conjugated
Biotin	CSB-PA617899LD01HU	CKAP5 Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC, IF

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:200-1:500
IF	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Binds to the plus end of microtubules and regulates microtubule dynamics and microtubule organization. Acts as processive microtubule polymerase. Promotes cytoplasmic microtubule nucleation and elongation. Plays a major role in organizing spindle poles. In spindle formation protects kinetochore microtubules from depolymerization by KIF2C and has an essential role in centrosomal microtubule assembly independently of KIF2C activity. Contributes to centrosome integrity. Acts as component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge. The TACC3/ch-TOG/clathrin complex is required for the maintenance of kinetochore fiber tension. Enhances the strength of NDC80 complex-mediated kinetochore-tip microtubule attachments.
基因功能參考文獻：
1. Eribulin binding to the tip of microtubules and subsequent loss of ch-TOG is a priming event leading to alterations in microtubule dynamics and breast cancer cell migration. PMID: 26497677
2. Data show that cytoskeleton associated protein 5 (chTOG) only weakly promotes importin-regulated microtubule nucleation, but acts synergistically with microtubule- associated protein TPX2. PMID: 26414402
3. Aurora-A kinase does not regulate TACC3-chTOG complex formation, indicating that Aurora-A solely functions as a recruitment factor for the TACC3-chTOG complex to centrosomes and proximal mitotic spindles. PMID: 24273164
4. Data show that five genes CKAP5, KPNB1, RAN, TPX2 and KIF11 were shown to be essential for tumor cell survival in both head and neck squamous cell carcinoma (HNSCC)and non-small cell lung cancer (NSCLC), but most particularly in HNSCC. PMID: 23444224
5. These observations indicate that EB1 and ch-TOG regulate microtubule organisation differently via distinct regions in the plus ends of microtubules. PMID: 23251535
6. Clathrin promotes centrosome maturation by stabilizing the microtubule-binding protein ch-TOG, defining a novel role for the clathrin-ch-TOG complex. PMID: 22891263
7. Data show that ILK performs its centrosome clustering activity in a centrosome-dependent, manner through the microtubule regulating proteins TACC3 and ch-TOG. PMID: 20838383
8. the association between aurora A phosphorylation and spindle apparatus; regulation from aurora A is mediated by CHC in recruiting phospho-TACC3 and subsequently ch-TOG to mitotic spindles. PMID: 20566684
9. TOG has roles during mitosis in focusing MT minus ends at spindle poles, maintaining centrosome integrity, and contributing to spindle bipolarity PMID: 14718566
10. TOG mediates the association of hnRNP A2-positive granules with microtubules during transport and/or localization PMID: 15703215
11. Hsp90 is required to localize ch-TOG to the mitotic spindle. PMID: 17376965
12. A novel function of Aurora-A, the regulation of ch-TOG and MCAK localization, in a common pathway in control of spindle pole integrity. PMID: 18663358
13. ch-Tog has at least two distinct roles in spindle formation: it protects kinetochore microtubules from depolymerization by MCAK, and ch-Tog plays an essential role in centrosomal microtubule assembly, a function independent of MCAK activity. PMID: 18809577
14. Data hypothesize that TOGp is required for chromosome motility as a downstream consequence of reduced microtubule dynamics and/or density. PMID: 19373773
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亞細胞定位：

Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. Cytoplasm, cytoskeleton, spindle. Chromosome, centromere, kinetochore.
蛋白家族：

TOG/XMAP215 family
組織特異性：

Overexpressed in hepatomas and colonic tumors. Also expressed in skeletal muscle, brain, heart, placenta, lung, liver, kidney and pancreas. Expression is elevated in the brain; highly expressed in the Purkinje cell bodies of the cerebellum.
數據庫鏈接：

HGNC: 28959

OMIM: 611142

KEGG: hsa:9793

STRING: 9606.ENSP00000432768

UniGene: Hs.201253

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