CKAP4 Antibody

說明書

中文名稱：

CKAP4兔多克隆抗體
貨號：

CSB-PA133590
規格：

￥1100
圖片：
The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using CSB-PA133590(CKAP4 Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: ×200)

The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA133590(CKAP4 Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: ×200)

Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane 1-3: 293T cells, hepG2 cells, A549 cells, Primary antibody: CSB-PA133590(CKAP4 Antibody) at dilution 1/1250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds
其他：

產品詳情
產品評價
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靶點詳情

產品詳情

Uniprot No.：

Q07065
基因名：

CKAP4
別名：

63 kDa membrane protein antibody; CKAP 4 antibody; Ckap4 antibody; CKAP4_HUMAN antibody; Cytoskeleton associated protein 4 antibody; Cytoskeleton-associated protein 4 antibody; ERGIC 63 antibody; ERGIC63 antibody; MGC99554 antibody; p63 antibody; Transmembrane protein 63kD endoplasmic reticulum Golgi intermediate compartment antibody; Type II transmembrane protein p63 antibody
宿主：

Rabbit
反應種屬：

Human,Mouse
免疫原：

Synthetic peptide of Human CKAP4
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
抗體亞型：

IgG
純化方式：

Antigen affinity purification
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
產品提供形式：

Liquid
應用范圍：

ELISA,WB,IHC

推薦稀釋比：

Application	Recommended Dilution
ELISA	1:2000-1:10000
WB	1:1000-1:5000
IHC	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Mediates the anchoring of the endoplasmic reticulum to microtubules.; High-affinity epithelial cell surface receptor for the FZD8-related low molecular weight sialoglycopeptide APF/antiproliferative factor. Mediates the APF antiproliferative signaling within cells.
基因功能參考文獻：
1. Both DKK1 and CKAP4 are frequently expressed in pancreatic and lung tumours, and their simultaneous expression is negatively correlated with prognosis. PMID: 28514532
2. CKAP4 has a role as a Dickkopf1 receptor and in pancreatic and lung tumor progression PMID: 27322059
3. Human Prostate Basal cell hyperplasia is an expansion of p63. PMID: 28795417
4. APF binds specifically to sites within the cytoskeleton-associated protein 4 (CKAP4) extracellular domain PMID: 28893174
5. The still rudimentary information of how CLIMP-63 fulfills these different roles, what these are exactly and how post-translational modifications control them, will be discussed. PMID: 25849921
6. Although VIMP can interact with CLIMP-63 and Syn5L, it does not interact with MT-binding ER proteins (such as Reep1) that shape the tubular smooth ER PMID: 25008318
7. revealed that CKAP4 could associate with EGFR at basal status and the complex was reduced upon EGF stimulation, leading to release EGFR into cytoplasm PMID: 24838946
8. Single nucleotide polymorphisms in p63 are implicated in the etiology of nonsyndromic bladder-exstrophy-epispadias complex. PMID: 23913486
9. Here we report that the combination of p63, a master regulator of epidermal development and differentiation, and KLF4, a regulator of epidermal differentiation, is sufficient to convert dermal fibroblasts to a keratinocyte phenotype. PMID: 23921950
10. CKAP4 was correlated with favorable clinical outcome and was an independent predictor for overall survival in hepatic cholangiocarcinoma patients. PMID: 23665508
11. p63 positively regulates desmosome adhesion by directly controlling the expression of several desmosome genes, including Dsp, Dsc3 and Dsg1. PMID: 23108156
12. CLIMP63 regulates the luminal diameter within the endoplasmic reticulum and may play a role in regulating the formation of endoplasmic reticulum sheets. PMID: 21111237
13. The results define CLIMP-63 as a novel protein interactor and regulator of Dicer function, involved in maintaining Dicer protein levels in human cells. PMID: 23047949
14. Treatment of airway epitheial cells (AEC) with SP-A, monoclonal antibodies to CKAP4/P63, or CKAP4/P63-specific small interfering RNA decreased the binding of purified alginate exopolysaccharide to AEC. PMID: 22966120
15. This study identifies specific changes in skin structural biology and signalling pathways that result from mutant p63 and provides new molecular insight into the AEC syndrome phenotype PMID: 22329826
16. CCN2 regulation by antiproliferative factor involves CKAP4 nuclear translocation and binding to the CCN2 promoter. PMID: 22438586
17. Tumor markers, p63 and high molecular weight cytokeratin, may be utilised in the distinction between urothelial carcinoma with prostatic stromal invasion and urothelial carcinoma with colonisation of prostatic ducts and acini. PMID: 22406481
18. These findings strongly support the routine use of p40 in place of p63 for the diagnosis of pulmonary squamous cell carcinoma. PMID: 22056955
19. This study found a consistent expression profile and localization analysis of p63 and GM1 in primary keratinocytes and in human epidermal biopsies. PMID: 21820419
20. Telomere length in Sjogren syndrome is shorter and associated with lower levels of expression of p63 and nucleostemin than in non-Sjogren syndrome. PMID: 21655359
21. Synthetic as-antiproliferative factor inhibits cell proliferation in T24 bladder carcinoma cells via the CKAP4 receptor PMID: 21143984
22. Data demonstrate an important role for the PI3-kinase-Akt pathway in intracellular transport of surfactant protein A receptor P63. PMID: 20870746
23. p63 binds to an enhancer element in the SHFM1 locus and this element controls expression of DLX6 and DLX5 which are important for limb development. PMID: 20808887
24. p63 was not found to be highly sensitive for squamous cell carcinoma PMID: 20184665
25. I510T mutation of p63 was detected in both RHS and AEC syndrome patients and mutation of S541 residue can lead to either RHS (S541Y) or AEC syndrome (S541F). PMID: 20156774
26. evidence to support the hypothesis that p63 is the functional tPA binding site on VSMC PMID: 12913003
27. The ectrodactyly, absence of radius side part palm and split foot malformation are caused by the mutation of base pair at number 665 of the exon 5 of P63. PMID: 15476176
28. CLIMP-63-mediated stable anchoring of the endoplasmic reticulum (ER) to microtubules is required to maintain the spatial distribution of the ER during interphase. PMID: 15703217
29. is not responsible for autosomal-dominant amelogenesis imperfecta PMID: 16546853
30. cytoskeleton-associated protein 4/p63 (CKAP4/p63), a type II transmembrane receptor, binds with high affinity to APF PMID: 17030514
31. Olomoucine, a CDK inhibitor, up-regulates CKAP4, a cytoskeleton-linking membrane protein. PMID: 17975794
32. Identification of CKAP4/p63 as a substrate of DHHC2, a putative tumor suppressor. PMID: 18296695
33. CK MNF116 and p63 were useful in identifying squamous cell carcinomas with single cell infiltration. PMID: 18333895
34. Results substantiate the role of P63 as a lung surfactant protein-A receptor protein localized on the surface of lung type II cells. PMID: 18708633
35. DHHC2-mediated palmitoylation of CKAP4 has a role in opposing cancer-related cellular behaviors. PMID: 19144824
36. p73 and p63, but not p53, are modulated during the cell cycle PMID: 19861536
37. The p62/p63 protein was first identified because it is palmitoylated during mitosis. PMID: 1730740
38. Purification and cloning of p62/p63. PMID: 8566419
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亞細胞定位：

Endoplasmic reticulum membrane; Single-pass type II membrane protein. Cell membrane; Single-pass type II membrane protein. Cytoplasm, cytoskeleton. Cytoplasm, perinuclear region. Note=Translocates to the perinuclear region upon APF-stimulation.
數據庫鏈接：

HGNC: 16991

KEGG: hsa:10970

STRING: 9606.ENSP00000367265

UniGene: Hs.74368

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