CHTOP Antibody

中文名稱：

CHTOP兔多克隆抗體
貨號：

CSB-PA897109HA01HU
規(guī)格：

￥440
圖片：
Western blot
All lanes: CHTOP antibody at 14 μg/ml
Lane 1: Mouse heart tissue
Lane 2: Mouse kidney tissue
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 27, 22 kDa
Observed band size: 27, 52 kDa

Immunohistochemistry of paraffin-embedded human endometrial cancer using CSB-PA897109HA01HU at dilution of 1:100

Immunohistochemistry of paraffin-embedded human glioma using CSB-PA897109HA01HU at dilution of 1:100
其他：

產(chǎn)品詳情
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

產(chǎn)品描述：

The peptide corresponding to amino acids 179-248 of the recombinant human CHTOP is used to immunize rabbits to produce the anti-CHTOP antibody. The resulting antibody is a polyclonal antibody and occurs as an unconjugated IgG. Its purity is over 95% using protein G purified. It only reacts with human and mouse CHTOP protein, which is involved in the activation of estrogen–receptor target genes, the downregulation of fetal γ-globin during the developmental transition from fetal to adult hemoglobin, and mRNA export. This CHTOP antibody is suitable for the detection of CHTOP protein in ELISA, WB, and IHC applications.
產(chǎn)品名稱：

Rabbit anti-Homo sapiens (Human) CHTOP Polyclonal antibody
Uniprot No.：

Q9Y3Y2
基因名：

CHTOP
別名：

C1orf77 antibody; chromatin target of chromatin target of PRMT1 protein antibody; Chromatin target of PRMT1 antibody; Chromatin target of PRMT1 protein antibody; Chromosome 1 open reading frame 77 antibody; CHTOP antibody; CHTOP_HUMAN antibody; DKFZp547E1010 antibody; FL- RP1-178F15.2 antibody; FL-SRAG antibody; FOP antibody; Friend of PRMT1 antibody; Friend of PRMT1 protein antibody; HT031 antibody; MGC131924 antibody; MGC86949 antibody; OTTHUMP00000035122 antibody; OTTHUMP00000035123 antibody; Pp7704 antibody; RP1 178F15.2 antibody; Small arginine- and glycine-rich protein antibody; Small protein rich in arginine and glycine antibody; SRAG antibody; SRAG-3 antibody; SRAG-5 antibody
宿主：

Rabbit
反應(yīng)種屬：

Human, Mouse
免疫原：

Recombinant Human Chromatin target of PRMT1 protein (179-248AA)
免疫原種屬：

Homo sapiens (Human)

標(biāo)記方式：

Non-conjugated

本頁面中的產(chǎn)品，CHTOP Antibody (CSB-PA897109HA01HU)，的標(biāo)記方式是Non-conjugated。對于CHTOP Antibody，我們還提供其他標(biāo)記。見下表：

標(biāo)記方式	貨號	產(chǎn)品名稱	應(yīng)用
HRP	CSB-PA897109HB01HU	CHTOP Antibody, HRP conjugated	ELISA
FITC	CSB-PA897109HC01HU	CHTOP Antibody, FITC conjugated
Biotin	CSB-PA897109HD01HU	CHTOP Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, WB, IHC

推薦稀釋比：

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:20-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點(diǎn)詳情

功能：

Plays an important role in the ligand-dependent activation of estrogen receptor target genes. May play a role in the silencing of fetal globin genes. Recruits the 5FMC complex to ZNF148, leading to desumoylation of ZNF148 and subsequent transactivation of ZNF148 target genes. Plays an important role in the tumorigenicity of glioblastoma cells. Binds to 5-hydroxymethylcytosine (5hmC) and associates with the methylosome complex containing PRMT1, PRMT5, MEP50 and ERH. The CHTOP-methylosome complex associated with 5hmC is recruited to selective sites on the chromosome, where it methylates H4R3 and activates the transcription of genes involved in glioblastomagenesis.; Required for effective mRNA nuclear export and is a component of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. Stimulates DDX39B ATPase and helicase activities. In cooperation with ALYREF/THOC4 enhances NXF1 RNA binding activity.
基因功能參考文獻(xiàn)：
1. the cellular level of Chtop is autoregulated via Int2 retention of its own mRNA, which subsequently undergoes NMD. PMID: 27683223
2. Results suggest that 5hmC plays a critical role in glioblastomagenesis by recruiting the CHTOP-methylosome complex to selective sites on the chromosome, where it methylates H4R3 and activates the transcription of cancer-related genes. PMID: 25284789
3. Chtop is a component of the dynamic TREX mRNA export complex that, along with Alyref, activates the ATPase and RNA helicase activities of Uap56. PMID: 23299939
4. The recently identified chromatin factor Friend of Prmt1 (FOP) is a critical modulator of gamma-globin gene expression. PMID: 20688955
5. Fop is tightly associated with chromatin, and that it is modified by both asymmetric and symmetric arginine methylation in vivo. Fop plays an important role in the ligand-dependent activation of estrogen receptor target genes, including TFF1 (pS2). PMID: 19858291
6. The reduction in SRAG protein that occurs in proliferating cells was mapped with inhibitors to the G(2)/M phase of the cell cycle. As expected, the overexpression of SRAG reduced the percentage of cells in the G(2)/M phase and increased cell death. PMID: 19254951
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亞細(xì)胞定位：

Nucleus. Nucleus, nucleolus. Nucleus, nucleoplasm. Nucleus speckle.
組織特異性：

Expressed in an erythroid progenitor cell line derived from peripheral blood. Expressed in glioblastoma cells.
數(shù)據(jù)庫鏈接：

HGNC: 24511

OMIM: 614206

KEGG: hsa:26097

STRING: 9606.ENSP00000357683

UniGene: Hs.611057

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