CCNA1 Antibody

說明書

中文名稱：

CCNA1兔多克隆抗體
貨號：

CSB-PA574179
規格：

￥2024
圖片：
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Cyclin A antibody.

Immunofluorescence analysis of COS7 cells, using Cyclin A antibody.

Western blot analysis of extracts from COS7 cells, using Cyclin A antibody.

Western blot analysis of extracts from JK cells (Lane 2) and cos-7 cells (Lane 3), using Cyclin A antiobdy. The lane on the left is treated with synthesized peptide.
其他：

產品詳情
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靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) CCNA1 Polyclonal antibody
Uniprot No.：

P78396
基因名：

CCNA1
宿主：

Rabbit
反應種屬：

Human,Mouse
免疫原：

Synthesized peptide derived from Human Cyclin A.
免疫原種屬：

Homo sapiens (Human)
克隆類型：

Polyclonal
純化方式：

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
濃度：

It differs from different batches. Please contact us to confirm it.
產品提供形式：

Liquid
應用范圍：

ELISA,WB,IHC,IF

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:3000
IHC	1:50-1:100
IF	1:100-1:500

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

May be involved in the control of the cell cycle at the G1/S (start) and G2/M (mitosis) transitions. May primarily function in the control of the germline meiotic cell cycle and additionally in the control of mitotic cell cycle in some somatic cells.
基因功能參考文獻：
1. Thus, Cyclin A/Cdk1 phosphorylation primes MYPT1 for Plk1 binding. These data demonstrate cross-regulation between Cyclin A/Cdk1-dependent and Plk1-dependent phosphorylation of substrates during mitosis to ensure efficient correction of kinetochore microtubule attachment errors necessary for high mitotic fidelity. PMID: 29154753
2. Cyclin A1 functions as an oncogene that controls proliferative and survival activities in tumourigenesis and chemoresistance of ovarian cancer. PMID: 27669502
3. In the bone marrow, cyclin A1 and aromatase enhanced local bone marrow-releasing factors, including androgen receptor, estrogen and matrix metalloproteinase MMP9 and promoted the metastatic growth of prostate cancer cells PMID: 26921336
4. The data showed that E7 induced CCNA1 methylation by forming a complex with Dnmt1 at the CCNA1 promoter, resulting in the subsequent reduction of expression in cancers. PMID: 26250467
5. DNA methylation status of key cell-cycle regulators such as CDKNA2/p16 and CCNA1 correlates with treatment response to doxorubicin and 5-fluorouracil in locally advanced breast tumors. PMID: 25294903
6. These data from results indicated a significant connection of CCNA1 methylation with poor progression in human malignant tumors among both Caucasian and Asian populations. PMID: 25654082
7. Cyclin A1 immunoexpression is of potential utility in predicting disease progression in patients with pT1 urothelial carcinomas of the bladder PMID: 25039670
8. HS3ST2 and CCNA1 genes may play important roles in HPV-induced cervical cancer and patients with specific hypermethylated genes may have a greater risk of progressing to invasive cervical cancer. PMID: 25198553
9. CCNA1 promoter methylation is associated with cervical squamous intraepithelial lesions. PMID: 25292097
10. High CCNA1 expression is associated with nasopharyngeal carcinoma. PMID: 25265349
11. Mutations in genes such as AKT2, CCNA1, MAP3K4, and TGFBR1, were associated significantly with Epstein-Barr-positive gastric tumors, compared with EBV-negative tumors. PMID: 25173755
12. Promoter reporter assays with a series of deletion constructs determined that the DNA element from -102 to -96 bp of the cyclin A1 promoter is responsible for PITX2-induced gene expression. PMID: 24002705
13. presents CCNA1 and TIMP3 hypermethylation as a helpful tool to identify HNSCC subjects at risk of developing second primary carcinomas PMID: 24359512
14. Overexpression of cyclin A1 in FSHD indicates cell cycle dysregulation in FSHD and might contribute to clinical symptoms of this disease PMID: 24019929
15. Nuclear FoxO3a promotes cell cycle progression by transcriptional upregulation of cyclin A1, promoting proliferation of human anaplastic thyroid carcinoma cells. PMID: 22718346
16. These findings indicate the possible involvement of cyclins A and E in the pathogenesis of malignant melanoma. PMID: 22763964
17. The organization of actin and cytokeratin cytoskeleton and the expression of TCTP, p53,cyclin A, RhoA and actin in HIO180 non-transformed ovarian epithelial cells, and OVCAR3 and SKOV3 ovarian epithelial cancer cells, was studied. PMID: 23042265
18. Cyclin A1 as overexpressed in induced lpuripotent stem cells cells compared to embryonic stem cells, suggesting that during reprogramming, cyclin A1 protein expression levels are not set correctly. PMID: 22500553
19. Cyclin A1 is an important cell cycle regulator with age-related increased expression in tonsils of children. HPV16 induces overexpression of Cyclin A1 in HNSCC despite promoter methylation. PMID: 22712549
20. Increased cyclin A1 in human bladder cancer cells may be regulated by activity of the USP2a deubiquitinase. PMID: 22370483
21. Cyclin-A1 is the first prototypic leukemia-testis-antigen to be expressed in acute myeloid leukemia leukemic stem cells. PMID: 22529286
22. Oxidized low density lipoprotein induces cyclin A synthesis in a process involving ERK, JNK and NFkappaB PMID: 21764057
23. The integrated form of HPV might lead to CCNA1 promoter methylation in cervical cancer by some mechanisms. PMID: 21412159
24. CCNA1 methylation may play a crucial role in HPV16-induced carcinogenesis of HNSCC independently of p53 PMID: 21563216
25. anti-oncogenic role of miR-372 may be through control of cell growth and cell cycle progression by down-regulating the cell cycle genes CDK2 and cyclin A1 PMID: 21646351
26. mRNA expression of cyclin A1 in myelodysplastic syndrome patients is higher than in normal controls. PMID: 19379570
27. An increase in cyclin A1 expression was the only differentially expressed cell cycle regulatory gene found. Greater cyclin A protein levels were consistently observed in cells with active IRE1alpha and were dependent on XBP-1. PMID: 20013084
28. revealed that KDM8 occupies the coding region of cyclin A1 and directly regulates transcription. PMID: 20457893
29. These results indicate that HIV-1 integrase is required during uncoating for maintaining CypA-capsid protein interaction, which promotes optimal stability of the viral core. PMID: 20219923
30. Methylation of CCNA1 in cervical scrapings is strongly associated with high-grade cervical intraepithelial neoplasia and cervical cancer. PMID: 19843677
31. Four point mutations, c.321T>C, IVS3 +32G>C, IVS5+38A>G and c.1158G>A, are not associated with spermatogenesis impairment. PMID: 19886767
32. the level of cyclin A1 mRNA expression predicts meiotic disorders during spermatogenesis PMID: 12121569
33. CCNA1 had successive increases in its promoter activity during spermatgogensis in transgenic mice. PMID: 12579332
34. study provides evidence that the cyclin A1-cyclin dependent kinase 2 complex plays a role in several signaling pathways important for cell cycle control and meiosis PMID: 15159402
35. Cyclin A1 methylation was inversely related to p53 mutational status in primary tumors, and forced expression of cyclin A1 resulted in robust induction of wild-type p53 in HNSCC cell lines. PMID: 15342377
36. These findings establish a novel function for cyclin A1 and CDK2 in DNA double strand break repair following radiation damage. PMID: 15456866
37. these analyses demonstrate that cyclin A1 contributes to G1 to S cell cycle progression in somatic cells. PMID: 15829981
38. cyclin A1 mediates VEGF expression in cooperation with Rb- and androgen-dependent pathways in prostate cancer PMID: 16007189
39. Significant tumour specific methylation of cyclin A1 promoter seen in oral sqmaous cell carcinoma PMID: 16449996
40. The data implicate cyclin A1 as a downstream player in p53-dependent apoptosis and G2 arrest. PMID: 16799873
41. Mip/LIN-9 is required for the expression of B-Myb, and both proteins collaborate in the control of the cell cycle progression via the regulation of S phase and cyclin A, cyclin B, and CDK1 PMID: 17098733
42. these analyses demonstrate that cyclin A1 exerts antiapoptotic functions by interacting with retinoblastoma and Ku proteins in leukemia cells. PMID: 17455244
43. Over expression of H179Y-mutant p53 promoted G1 to S phase transition with enlarged cell size and increased cyclin A1 and Cdk4 expression in HELF cells. PMID: 17530187
44. G2 phase cyclin A/cdk2 controls the timing of entry into mitosis by controlling the subsequent activation of cyclin B/cdk1, but also has an unexpected role in coordinating the activation of cyclin B/cdk1 at the centrosome and in the nucleus PMID: 18372919
45. Cyclin A1 contributes to prostate cancer invasion by modulating the expression of MMPs and VEGF and by interacting with AR (androgen receptor). PMID: 18612129
46. The CCNA1, CCNB1, CCNB2, PRM1, and PRM2 messenger RNA transcript ratios were significantly decreased in patients with spermatogenic disorders. Transcript ratios in patients with successful sperm retrieval were higher than with failed sperm retrieval. PMID: 18692784
47. Results suggest that cyclin A1 protein is stabilized via post-transcriptional modification in response to apoptosis induced by staurosporine or TNFalpha. PMID: 18787932
48. Cyclin A1- and cyclin A2-containing CDK complexes were compared in vitro by determining kinetic constants and by examining the complexes for their ability to phosphorylate pRb and p53. PMID: 19056339
49. Study shows that transcription corepressor CtBP2 directly binds acinus, which is regulated by nerve growth factor (NGF), inhibiting its stimulatory effect on cyclin A1, but not cyclin A2, expression in leukemia. PMID: 19668232
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亞細胞定位：

Nucleus.
蛋白家族：

Cyclin family, Cyclin AB subfamily
組織特異性：

Very high levels in testis and very low levels in brain. Also found in myeloid leukemia cell lines.
數據庫鏈接：

HGNC: 1577

OMIM: 604036

KEGG: hsa:8900

STRING: 9606.ENSP00000255465

UniGene: Hs.417050

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