CARMIL2 Antibody

中文名稱：

CARMIL2兔多克隆抗體
貨號：

CSB-PA739364LA01HU
規格：

￥440
圖片：
Western Blot
Positive WB detected in: Jurkat whole cell lysate
All lanes: CARMIL2 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 155, 149 kDa
Observed band size: 155 kDa

Immunoprecipitating CARMIL2 in Jurkat whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA739364LA01HU in Jurkat whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA739364LA01HU (6μg) + Jurkat whole cell lysate (500μg)
Lane 3: Jurkat whole cell lysate (20μg)
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) CARMIL2 Polyclonal antibody
Uniprot No.：

Q6F5E8
基因名：

CARMIL2
別名：

CARMIL2 antibody; CARMIL2b antibody; Leucine rich repeat containing 16C antibody; leucine-rich repeat antibody; Leucine-rich repeat-containing protein 16C antibody; LR16C_HUMAN antibody; LRRC16C antibody; RGD antibody; RGD leucine rich repeat tropomodulin and proline rich containing protein antibody; RGD motif leucine rich repeats tropomodulin domain and proline rich containing antibody; Rltpr antibody; tropomodulin and proline-rich-containing protein antibody
宿主：

Rabbit
反應種屬：

Human
免疫原：

Recombinant Human Capping protein, Arp2/3 and myosin-I linker protein 2 protein (1136-1290AA)
免疫原種屬：

Homo sapiens (Human)

標記方式：

Non-conjugated

本頁面中的產品，CARMIL2 Antibody (CSB-PA739364LA01HU)，的標記方式是Non-conjugated。對于CARMIL2 Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產品名稱	應用
HRP	CSB-PA739364LB01HU	CARMIL2 Antibody, HRP conjugated	ELISA
FITC	CSB-PA739364LC01HU	CARMIL2 Antibody, FITC conjugated
Biotin	CSB-PA739364LD01HU	CARMIL2 Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IP

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:2000
IP	1:200-1:2000

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunoprecipitation (IP) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Cell membrane-cytoskeleton-associated protein that plays a role in the regulation of actin polymerization at the barbed end of actin filaments. Prevents F-actin heterodimeric capping protein (CP) activity at the leading edges of migrating cells, and hence generates uncapped barbed ends and enhances actin polymerization. Plays a role in cell protrusion formations; involved in cell polarity, lamellipodial assembly, membrane ruffling and macropinosome formations. Involved as well in cell migration and invadopodia formation during wound healing. Required for CD28-mediated stimulation of NF-kappa-B signaling, involved in naive T cells activation, maturation into T memory cells, and differentiation into T helper and T regulatory cells.
基因功能參考文獻：
1. These cases showed that CARMIL2-deficiency is an autosomal recessive primary immunodeficiency disorder associated with defective CD28-mediated TCR co-signalling and impaired cytoskeletal dynamics. PMID: 28112205
2. RLTPR (p.Q575E) increases binding of RLTPR to downstream components of the NF-kappaB signaling pathway, selectively upregulates the NF-kappaB pathway in activated T cells. PMID: 28694326
3. the scaffolding role of RLTPR predominates during CD28 co-stimulation and underpins the similar function of RLTPR in human and mouse T cells. PMID: 27647348
4. Human RLTPR deficiency is a Combined immunodeficiency affecting at least the CD28-responsive pathway in T cells and the BCR-responsive pathway in B cells. PMID: 27647349
5. On the basis of these results, we propose a model in which dynamic vimentin filaments target CARMIL2 to critical membrane-associated locations, where CARMIL2 regulates CP, and thus actin assembly, to create cell protrusions PMID: 26466680
6. Data suggest that CARMIL2 displays two localization patterns in cells; one coincides with vimentin intermediate filaments, and the other coincides with cell membrane at leading edge of migrating cells. PMID: 26578515
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亞細胞定位：

[Isoform 2]: Cytoplasm. Cytoplasm, cytoskeleton. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, lamellipodium. Cell projection, ruffle.
蛋白家族：

CARMIL family
組織特異性：

Expressed in all tissues tested, including thymus, spleen, colon, leukocytes, peripheral blood, skin, skin keratinocytes and skin fibroblasts. Strong expression is detected in naive and memory CD4+ and CD8+ T cells, naive and memory B cells, regulatory T
數據庫鏈接：

HGNC: 27089

OMIM: 610859

KEGG: hsa:146206

STRING: 9606.ENSP00000334958

UniGene: Hs.611432

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